3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression

Alex Ismail, Nadia Ayala-Lopez, Maleeha Ahmad, Stephanie W. Watts

    Research output: Research - peer-reviewArticle

    Abstract

    Rat perivascular adipose tissue (PVAT) stores, takes up, and releases norepinephrine (NE; Ayala-Lopez et al. (2014) Pharmacol Res Perspect 2, e00041). We hypothesized that 3T3-L1 adipocytes would exhibit similar behaviors and, thus, could serve as a model for PVAT adipocytes. However, basal levels of NE were not detected in 3T3-L1 adipocytes. While incubation of 3T3-L1 adipocytes with exogenous NE increased their cellular NE content, the mRNA expression of several NE transporters [e.g., norepinephrine transporter (NET)] were not detected in these cells. Similarly, we observed expression of the vesicular monoamine transporter 1 (VMAT1) in 3T3-L1 adipocytes by qRT-PCR and immunostaining, but stimulation of the cells with tyramine (100 μm) did not cause a significant release of NE. These studies support that 3T3-L1 adipocytes are not an adequate model of perivascular adipocytes for studying NE handling.

    LanguageEnglish (US)
    Pages137-144
    Number of pages8
    JournalFEBS Letters
    Volume591
    Issue number1
    DOIs
    StatePublished - Jan 1 2017

    Profile

    Amines
    Tissue
    3T3-L1 Cells
    Adipocytes
    Vesicular Monoamine Transport Proteins
    Norepinephrine Plasma Membrane Transport Proteins
    Tyramine
    Rats
    Norepinephrine
    Messenger RNA
    Adipose Tissue
    Polymerase Chain Reaction
    Handling (Psychology)

    Keywords

    • 3T3-L1 adipocytes
    • catecholamines
    • obesity
    • perivascular adipose tissue

    ASJC Scopus subject areas

    • Structural Biology
    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

    Cite this

    3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression. / Ismail, Alex; Ayala-Lopez, Nadia; Ahmad, Maleeha; Watts, Stephanie W.

    In: FEBS Letters, Vol. 591, No. 1, 01.01.2017, p. 137-144.

    Research output: Research - peer-reviewArticle

    Ismail, Alex ; Ayala-Lopez, Nadia ; Ahmad, Maleeha ; Watts, Stephanie W./ 3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression. In: FEBS Letters. 2017 ; Vol. 591, No. 1. pp. 137-144
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