3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression

Alex Ismail, Nadia Ayala-Lopez, Maleeha Ahmad, Stephanie W. Watts

Research output: Contribution to journalArticle

Abstract

Rat perivascular adipose tissue (PVAT) stores, takes up, and releases norepinephrine (NE; Ayala-Lopez et al. (2014) Pharmacol Res Perspect 2, e00041). We hypothesized that 3T3-L1 adipocytes would exhibit similar behaviors and, thus, could serve as a model for PVAT adipocytes. However, basal levels of NE were not detected in 3T3-L1 adipocytes. While incubation of 3T3-L1 adipocytes with exogenous NE increased their cellular NE content, the mRNA expression of several NE transporters [e.g., norepinephrine transporter (NET)] were not detected in these cells. Similarly, we observed expression of the vesicular monoamine transporter 1 (VMAT1) in 3T3-L1 adipocytes by qRT-PCR and immunostaining, but stimulation of the cells with tyramine (100 μm) did not cause a significant release of NE. These studies support that 3T3-L1 adipocytes are not an adequate model of perivascular adipocytes for studying NE handling.

LanguageEnglish (US)
Pages137-144
Number of pages8
JournalFEBS Letters
Volume591
Issue number1
DOIs
StatePublished - Jan 1 2017

Profile

3T3-L1 Cells
Adipocytes
Amines
Vesicular Monoamine Transport Proteins
Norepinephrine Plasma Membrane Transport Proteins
Tissue
Tyramine
Rats
Norepinephrine
Messenger RNA
Adipose Tissue
Polymerase Chain Reaction

Keywords

  • 3T3-L1 adipocytes
  • catecholamines
  • obesity
  • perivascular adipose tissue

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression. / Ismail, Alex; Ayala-Lopez, Nadia; Ahmad, Maleeha; Watts, Stephanie W.

In: FEBS Letters, Vol. 591, No. 1, 01.01.2017, p. 137-144.

Research output: Contribution to journalArticle

Ismail, Alex ; Ayala-Lopez, Nadia ; Ahmad, Maleeha ; Watts, Stephanie W./ 3T3-L1 cells and perivascular adipocytes are not equivalent in amine transporter expression. In: FEBS Letters. 2017 ; Vol. 591, No. 1. pp. 137-144
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