5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots

Akiko Maruyama-Nakashita, Akiko Suyama, Hideki Takahashi

Research output: Contribution to journalComment/debate

Abstract

Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

LanguageEnglish (US)
Pages51-55
Number of pages5
JournalPlant Biotechnology
Volume34
Issue number1
DOIs
StatePublished - 2017

Profile

5' Untranslated Regions
5' untranslated regions
luciferase
Sulfur
Arabidopsis
sulfur
Luciferases
Sulfates
sulfates
Genetically Modified Plants
transgenic plants
Firefly Luciferases
uptake mechanisms
epidermis (plant)
Epidermis
transporters
cortex
Messenger RNA
Proteins
proteins

Keywords

  • 2
  • 5′UTR
  • Sulfate transporter
  • SULTR1
  • Translation
  • −S-inducible expression

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots",
abstract = "Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.",
keywords = "2, 5′UTR, Sulfate transporter, SULTR1, Translation, −S-inducible expression",
author = "Akiko Maruyama-Nakashita and Akiko Suyama and Hideki Takahashi",
year = "2017",
doi = "10.5511/plantbiotechnology.16.1226a",
language = "English (US)",
volume = "34",
pages = "51--55",
journal = "Plant Biotechnology",
issn = "1342-4580",
publisher = "Japanese Society for Plant Cell and Molecular Biology",
number = "1",

}

TY - JOUR

T1 - 5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots

AU - Maruyama-Nakashita,Akiko

AU - Suyama,Akiko

AU - Takahashi,Hideki

PY - 2017

Y1 - 2017

N2 - Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

AB - Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

KW - 2

KW - 5′UTR

KW - Sulfate transporter

KW - SULTR1

KW - Translation

KW - −S-inducible expression

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DO - 10.5511/plantbiotechnology.16.1226a

M3 - Comment/debate

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SP - 51

EP - 55

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