5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots

Akiko Maruyama-Nakashita, Akiko Suyama, Hideki Takahashi

    Research output: Research - peer-reviewComment/debate

    Abstract

    Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

    LanguageEnglish (US)
    Pages51-55
    Number of pages5
    JournalPlant Biotechnology
    Volume34
    Issue number1
    DOIs
    StatePublished - 2017

    Profile

    5' untranslated regions
    luciferase
    sulfur
    Arabidopsis
    5' Untranslated Regions
    Sulfur
    Luciferases
    sulfates
    Sulfates
    transgenic plants
    uptake mechanisms
    Genetically Modified Plants
    epidermis (plant)
    transporters
    cortex
    proteins
    Firefly Luciferases
    Epidermis
    Messenger RNA
    Proteins

    Keywords

    • 2
    • 5′UTR
    • Sulfate transporter
    • SULTR1
    • Translation
    • −S-inducible expression

    ASJC Scopus subject areas

    • Biotechnology
    • Agronomy and Crop Science
    • Plant Science

    Cite this

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    title = "5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots",
    abstract = "Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.",
    keywords = "2, 5′UTR, Sulfate transporter, SULTR1, Translation, −S-inducible expression",
    author = "Akiko Maruyama-Nakashita and Akiko Suyama and Hideki Takahashi",
    year = "2017",
    doi = "10.5511/plantbiotechnology.16.1226a",
    volume = "34",
    pages = "51--55",
    journal = "Plant Biotechnology",
    issn = "1342-4580",
    publisher = "Japanese Society for Plant Cell and Molecular Biology",
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    TY - JOUR

    T1 - 5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in arabidopsis roots

    AU - Maruyama-Nakashita,Akiko

    AU - Suyama,Akiko

    AU - Takahashi,Hideki

    PY - 2017

    Y1 - 2017

    N2 - Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

    AB - Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major high-affinity sulfate transporter induced in epidermis and cortex of roots for mediating sulfate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.

    KW - 2

    KW - 5′UTR

    KW - Sulfate transporter

    KW - SULTR1

    KW - Translation

    KW - −S-inducible expression

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