A roadmap for interpreting 13C metabolite labeling patterns from cells

Joerg M. Buescher, Maciek R. Antoniewicz, Laszlo G. Boros, Shawn C. Burgess, Henri Brunengraber, Clary B. Clish, Ralph J. DeBerardinis, Olivier Feron, Christian Frezza, Bart Ghesquiere, Eyal Gottlieb, Karsten Hiller, Russell G. Jones, Jurre J. Kamphorst, Richard G. Kibbey, Alec C. Kimmelman, Jason W. Locasale, Sophia Y. Lunt, Oliver D K Maddocks, Craig Malloy & 17 others Christian M. Metallo, Emmanuelle J. Meuillet, Joshua Munger, Katharina Nöh, Joshua D. Rabinowitz, Markus Ralser, Uwe Sauer, Gregory Stephanopoulos, Julie St-Pierre, Daniel A. Tennant, Christoph Wittmann, Matthew G. Vander Heiden, Alexei Vazquez, Karen Vousden, Jamey D. Young, Nicola Zamboni, Sarah Maria Fendt

Research output: Contribution to journalArticle

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Abstract

Measuring intracellular metabolism has increasingly led to important insights in biomedical research. 13C tracer analysis, although less information-rich than quantitative 13C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting 13C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.

LanguageEnglish (US)
Pages189-201
Number of pages13
JournalCurrent Opinion in Biotechnology
Volume34
DOIs
StatePublished - Aug 1 2015

Profile

Data integration
Metabolites
Metabolism
Labeling
Nutrients
Biomedical Research
Fluxes
Food
Experiments

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Buescher, J. M., Antoniewicz, M. R., Boros, L. G., Burgess, S. C., Brunengraber, H., Clish, C. B., ... Fendt, S. M. (2015). A roadmap for interpreting 13C metabolite labeling patterns from cells. Current Opinion in Biotechnology, 34, 189-201. DOI: 10.1016/j.copbio.2015.02.003

A roadmap for interpreting 13C metabolite labeling patterns from cells. / Buescher, Joerg M.; Antoniewicz, Maciek R.; Boros, Laszlo G.; Burgess, Shawn C.; Brunengraber, Henri; Clish, Clary B.; DeBerardinis, Ralph J.; Feron, Olivier; Frezza, Christian; Ghesquiere, Bart; Gottlieb, Eyal; Hiller, Karsten; Jones, Russell G.; Kamphorst, Jurre J.; Kibbey, Richard G.; Kimmelman, Alec C.; Locasale, Jason W.; Lunt, Sophia Y.; Maddocks, Oliver D K; Malloy, Craig; Metallo, Christian M.; Meuillet, Emmanuelle J.; Munger, Joshua; Nöh, Katharina; Rabinowitz, Joshua D.; Ralser, Markus; Sauer, Uwe; Stephanopoulos, Gregory; St-Pierre, Julie; Tennant, Daniel A.; Wittmann, Christoph; Vander Heiden, Matthew G.; Vazquez, Alexei; Vousden, Karen; Young, Jamey D.; Zamboni, Nicola; Fendt, Sarah Maria.

In: Current Opinion in Biotechnology, Vol. 34, 01.08.2015, p. 189-201.

Research output: Contribution to journalArticle

Buescher, JM, Antoniewicz, MR, Boros, LG, Burgess, SC, Brunengraber, H, Clish, CB, DeBerardinis, RJ, Feron, O, Frezza, C, Ghesquiere, B, Gottlieb, E, Hiller, K, Jones, RG, Kamphorst, JJ, Kibbey, RG, Kimmelman, AC, Locasale, JW, Lunt, SY, Maddocks, ODK, Malloy, C, Metallo, CM, Meuillet, EJ, Munger, J, Nöh, K, Rabinowitz, JD, Ralser, M, Sauer, U, Stephanopoulos, G, St-Pierre, J, Tennant, DA, Wittmann, C, Vander Heiden, MG, Vazquez, A, Vousden, K, Young, JD, Zamboni, N & Fendt, SM 2015, 'A roadmap for interpreting 13C metabolite labeling patterns from cells' Current Opinion in Biotechnology, vol. 34, pp. 189-201. DOI: 10.1016/j.copbio.2015.02.003
Buescher JM, Antoniewicz MR, Boros LG, Burgess SC, Brunengraber H, Clish CB et al. A roadmap for interpreting 13C metabolite labeling patterns from cells. Current Opinion in Biotechnology. 2015 Aug 1;34:189-201. Available from, DOI: 10.1016/j.copbio.2015.02.003
Buescher, Joerg M. ; Antoniewicz, Maciek R. ; Boros, Laszlo G. ; Burgess, Shawn C. ; Brunengraber, Henri ; Clish, Clary B. ; DeBerardinis, Ralph J. ; Feron, Olivier ; Frezza, Christian ; Ghesquiere, Bart ; Gottlieb, Eyal ; Hiller, Karsten ; Jones, Russell G. ; Kamphorst, Jurre J. ; Kibbey, Richard G. ; Kimmelman, Alec C. ; Locasale, Jason W. ; Lunt, Sophia Y. ; Maddocks, Oliver D K ; Malloy, Craig ; Metallo, Christian M. ; Meuillet, Emmanuelle J. ; Munger, Joshua ; Nöh, Katharina ; Rabinowitz, Joshua D. ; Ralser, Markus ; Sauer, Uwe ; Stephanopoulos, Gregory ; St-Pierre, Julie ; Tennant, Daniel A. ; Wittmann, Christoph ; Vander Heiden, Matthew G. ; Vazquez, Alexei ; Vousden, Karen ; Young, Jamey D. ; Zamboni, Nicola ; Fendt, Sarah Maria. / A roadmap for interpreting 13C metabolite labeling patterns from cells. In: Current Opinion in Biotechnology. 2015 ; Vol. 34. pp. 189-201
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