A roadmap for interpreting 13C metabolite labeling patterns from cells

Joerg M. Buescher, Maciek R. Antoniewicz, Laszlo G. Boros, Shawn C. Burgess, Henri Brunengraber, Clary B. Clish, Ralph J. DeBerardinis, Olivier Feron, Christian Frezza, Bart Ghesquiere, Eyal Gottlieb, Karsten Hiller, Russell G. Jones, Jurre J. Kamphorst, Richard G. Kibbey, Alec C. Kimmelman, Jason W. Locasale, Sophia Y. Lunt, Oliver D K Maddocks, Craig Malloy & 17 others Christian M. Metallo, Emmanuelle J. Meuillet, Joshua Munger, Katharina Nöh, Joshua D. Rabinowitz, Markus Ralser, Uwe Sauer, Gregory Stephanopoulos, Julie St-Pierre, Daniel A. Tennant, Christoph Wittmann, Matthew G. Vander Heiden, Alexei Vazquez, Karen Vousden, Jamey D. Young, Nicola Zamboni, Sarah Maria Fendt

    Research output: Research - peer-reviewArticle

    • 100 Citations

    Abstract

    Measuring intracellular metabolism has increasingly led to important insights in biomedical research. 13C tracer analysis, although less information-rich than quantitative 13C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting 13C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.

    LanguageEnglish (US)
    Pages189-201
    Number of pages13
    JournalCurrent Opinion in Biotechnology
    Volume34
    DOIs
    StatePublished - Aug 1 2015

    Profile

    Data integration
    Metabolites
    Metabolism
    Labeling
    Nutrients
    Fluxes
    Experiments
    Biomedical Research
    Food

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering

    Cite this

    Buescher, J. M., Antoniewicz, M. R., Boros, L. G., Burgess, S. C., Brunengraber, H., Clish, C. B., ... Fendt, S. M. (2015). A roadmap for interpreting 13C metabolite labeling patterns from cells. Current Opinion in Biotechnology, 34, 189-201. DOI: 10.1016/j.copbio.2015.02.003

    A roadmap for interpreting 13C metabolite labeling patterns from cells. / Buescher, Joerg M.; Antoniewicz, Maciek R.; Boros, Laszlo G.; Burgess, Shawn C.; Brunengraber, Henri; Clish, Clary B.; DeBerardinis, Ralph J.; Feron, Olivier; Frezza, Christian; Ghesquiere, Bart; Gottlieb, Eyal; Hiller, Karsten; Jones, Russell G.; Kamphorst, Jurre J.; Kibbey, Richard G.; Kimmelman, Alec C.; Locasale, Jason W.; Lunt, Sophia Y.; Maddocks, Oliver D K; Malloy, Craig; Metallo, Christian M.; Meuillet, Emmanuelle J.; Munger, Joshua; Nöh, Katharina; Rabinowitz, Joshua D.; Ralser, Markus; Sauer, Uwe; Stephanopoulos, Gregory; St-Pierre, Julie; Tennant, Daniel A.; Wittmann, Christoph; Vander Heiden, Matthew G.; Vazquez, Alexei; Vousden, Karen; Young, Jamey D.; Zamboni, Nicola; Fendt, Sarah Maria.

    In: Current Opinion in Biotechnology, Vol. 34, 01.08.2015, p. 189-201.

    Research output: Research - peer-reviewArticle

    Buescher, JM, Antoniewicz, MR, Boros, LG, Burgess, SC, Brunengraber, H, Clish, CB, DeBerardinis, RJ, Feron, O, Frezza, C, Ghesquiere, B, Gottlieb, E, Hiller, K, Jones, RG, Kamphorst, JJ, Kibbey, RG, Kimmelman, AC, Locasale, JW, Lunt, SY, Maddocks, ODK, Malloy, C, Metallo, CM, Meuillet, EJ, Munger, J, Nöh, K, Rabinowitz, JD, Ralser, M, Sauer, U, Stephanopoulos, G, St-Pierre, J, Tennant, DA, Wittmann, C, Vander Heiden, MG, Vazquez, A, Vousden, K, Young, JD, Zamboni, N & Fendt, SM 2015, 'A roadmap for interpreting 13C metabolite labeling patterns from cells' Current Opinion in Biotechnology, vol 34, pp. 189-201. DOI: 10.1016/j.copbio.2015.02.003
    Buescher JM, Antoniewicz MR, Boros LG, Burgess SC, Brunengraber H, Clish CB et al. A roadmap for interpreting 13C metabolite labeling patterns from cells. Current Opinion in Biotechnology. 2015 Aug 1;34:189-201. Available from, DOI: 10.1016/j.copbio.2015.02.003
    Buescher, Joerg M. ; Antoniewicz, Maciek R. ; Boros, Laszlo G. ; Burgess, Shawn C. ; Brunengraber, Henri ; Clish, Clary B. ; DeBerardinis, Ralph J. ; Feron, Olivier ; Frezza, Christian ; Ghesquiere, Bart ; Gottlieb, Eyal ; Hiller, Karsten ; Jones, Russell G. ; Kamphorst, Jurre J. ; Kibbey, Richard G. ; Kimmelman, Alec C. ; Locasale, Jason W. ; Lunt, Sophia Y. ; Maddocks, Oliver D K ; Malloy, Craig ; Metallo, Christian M. ; Meuillet, Emmanuelle J. ; Munger, Joshua ; Nöh, Katharina ; Rabinowitz, Joshua D. ; Ralser, Markus ; Sauer, Uwe ; Stephanopoulos, Gregory ; St-Pierre, Julie ; Tennant, Daniel A. ; Wittmann, Christoph ; Vander Heiden, Matthew G. ; Vazquez, Alexei ; Vousden, Karen ; Young, Jamey D. ; Zamboni, Nicola ; Fendt, Sarah Maria. / A roadmap for interpreting 13C metabolite labeling patterns from cells. In: Current Opinion in Biotechnology. 2015 ; Vol. 34. pp. 189-201
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