Adsorption and interaction of fibronectin and human serum albumin at the liquid-liquid interface

Sachin S. Vaidya, Robert Y. Ofoli

    Research output: Contribution to journalArticle

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    Abstract

    The goal of this work was to investigate the dynamics of human plasma fibronectin (HFN) at the oil-water interface and to characterize its interactions with human serum albumin (HSA) by total internal reflection fluorescence microscopy (TIRFM). Among key results, we observed that fibronectin adsorption at the oil-water interface is rapid and essentially irreversible, even over short time scales. This may be due to the highly flexible nature of the protein, which allows its various domains to quickly attain energetically favorable conformations. On the other hand, HSA adsorption at the oil-water interface is relatively reversible at short times, and the protein is readily displaced by fibronectin even after HSA has been adsorbed at the interface for as long as 2 h. At longer adsorption times, HSA is able to more effectively resist complete displacement by fibronectin, although we observed significant fibronectin adsorption even under those conditions. Displacement of adsorbed fibronectin by HSA was negligible under all conditions. Fibronectin also adsorbs preferentially from a mixture of HFN and HSA, even when the concentration of HSA is substantially higher. This study is relevant to such emerging research thrusts as the development of biomimetic interfaces for a variety of applications, where there is a clear need for better understanding of the effects of interfacial competition, adsorption time scales, and extent of adsorption irreversibility on interfacial dynamics.

    Original languageEnglish (US)
    Pages (from-to)5852-5858
    Number of pages7
    JournalLangmuir
    Volume21
    Issue number13
    DOIs
    StatePublished - Jun 21 2005

    Profile

    albumins
    serums
    Adsorption
    adsorption
    Water
    oils
    water
    Plasma (human)
    Proteins
    Liquids
    proteins
    interactions
    Pediococcus
    Birth Certificates
    Spontaneous Fractures
    Fluorescence microscopy
    Biomimetics
    Conformations
    liquid-liquid interfaces
    biomimetics

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

    Cite this

    Adsorption and interaction of fibronectin and human serum albumin at the liquid-liquid interface. / Vaidya, Sachin S.; Ofoli, Robert Y.

    In: Langmuir, Vol. 21, No. 13, 21.06.2005, p. 5852-5858.

    Research output: Contribution to journalArticle

    Vaidya, Sachin S.; Ofoli, Robert Y. / Adsorption and interaction of fibronectin and human serum albumin at the liquid-liquid interface.

    In: Langmuir, Vol. 21, No. 13, 21.06.2005, p. 5852-5858.

    Research output: Contribution to journalArticle

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