Adsorption and interaction of fibronectin and human serum albumin at the liquid-liquid interface

Sachin S. Vaidya, Robert Y. Ofoli

Research output: Contribution to journalArticle

  • 23 Citations

Abstract

The goal of this work was to investigate the dynamics of human plasma fibronectin (HFN) at the oil-water interface and to characterize its interactions with human serum albumin (HSA) by total internal reflection fluorescence microscopy (TIRFM). Among key results, we observed that fibronectin adsorption at the oil-water interface is rapid and essentially irreversible, even over short time scales. This may be due to the highly flexible nature of the protein, which allows its various domains to quickly attain energetically favorable conformations. On the other hand, HSA adsorption at the oil-water interface is relatively reversible at short times, and the protein is readily displaced by fibronectin even after HSA has been adsorbed at the interface for as long as 2 h. At longer adsorption times, HSA is able to more effectively resist complete displacement by fibronectin, although we observed significant fibronectin adsorption even under those conditions. Displacement of adsorbed fibronectin by HSA was negligible under all conditions. Fibronectin also adsorbs preferentially from a mixture of HFN and HSA, even when the concentration of HSA is substantially higher. This study is relevant to such emerging research thrusts as the development of biomimetic interfaces for a variety of applications, where there is a clear need for better understanding of the effects of interfacial competition, adsorption time scales, and extent of adsorption irreversibility on interfacial dynamics.

LanguageEnglish (US)
Pages5852-5858
Number of pages7
JournalLangmuir
Volume21
Issue number13
DOIs
StatePublished - Jun 21 2005

Profile

liquid-liquid interfaces
albumins
Fibronectins
Serum Albumin
serums
Adsorption
adsorption
Liquids
Plasma (human)
interactions
oils
Oils
Proteins
Water
water
proteins
Fluorescence microscopy
Biomimetics
biomimetics
Conformations

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Adsorption and interaction of fibronectin and human serum albumin at the liquid-liquid interface. / Vaidya, Sachin S.; Ofoli, Robert Y.

In: Langmuir, Vol. 21, No. 13, 21.06.2005, p. 5852-5858.

Research output: Contribution to journalArticle

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