An approach to enhance alignment and myelination of dorsal root ganglion neurons

Chun Liu, Christina Chan

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

Axon regeneration is a chaotic process due largely to unorganized axon alignment. Therefore, in order for a sufficient number of regenerated axons to bridge the lesion site, properly organized axonal alignment is required. Since demyelination after nerve injury strongly impairs the conductive capacity of surviving axons, remyelination is critical for successful functioning of regenerated nerves. Previously, we demonstrated that mesenchymal stem cells (MSCs) aligned on a pre-stretch induced anisotropic surface because the cells can sense a larger effective stiffness in the stretched direction than in the perpendicular direction. We also showed that an anisotropic surface arising from a mechanical pre-stretched surface similarly affects alignment, as well as growth and myelination of axons. Here, we provide a detailed protocol for preparing a pre-stretched anisotropic surface, the isolation and culture of dorsal root ganglion (DRG) neurons on a pre-stretched surface, and show the myelination behavior of a co-culture of DRG neurons with Schwann cells (SCs) on a pre-stretched surface.

LanguageEnglish (US)
Article numbere54085
JournalJournal of Visualized Experiments
Volume2016
Issue number114
DOIs
StatePublished - Aug 24 2016

Profile

Spinal Ganglia
Neurons
Axons
Schwann Cells
Demyelinating Diseases
Coculture Techniques
Mesenchymal Stromal Cells
Regeneration
Stem cells
Cell culture
Cells
Stiffness
Wounds and Injuries
Growth
Direction compound

Keywords

  • Anisotropy
  • Axon alignment
  • DRG isolation
  • Issue 114
  • Myelination
  • Neuroscience
  • Pre-stretch
  • Schwann cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

An approach to enhance alignment and myelination of dorsal root ganglion neurons. / Liu, Chun; Chan, Christina.

In: Journal of Visualized Experiments, Vol. 2016, No. 114, e54085, 24.08.2016.

Research output: Contribution to journalArticle

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