An approach to enhance alignment and myelination of dorsal root ganglion neurons

Chun Liu, Christina Chan

    Research output: Contribution to journalArticle

    • 1 Citations

    Abstract

    Axon regeneration is a chaotic process due largely to unorganized axon alignment. Therefore, in order for a sufficient number of regenerated axons to bridge the lesion site, properly organized axonal alignment is required. Since demyelination after nerve injury strongly impairs the conductive capacity of surviving axons, remyelination is critical for successful functioning of regenerated nerves. Previously, we demonstrated that mesenchymal stem cells (MSCs) aligned on a pre-stretch induced anisotropic surface because the cells can sense a larger effective stiffness in the stretched direction than in the perpendicular direction. We also showed that an anisotropic surface arising from a mechanical pre-stretched surface similarly affects alignment, as well as growth and myelination of axons. Here, we provide a detailed protocol for preparing a pre-stretched anisotropic surface, the isolation and culture of dorsal root ganglion (DRG) neurons on a pre-stretched surface, and show the myelination behavior of a co-culture of DRG neurons with Schwann cells (SCs) on a pre-stretched surface.

    Original languageEnglish (US)
    Article numbere54085
    JournalJournal of Visualized Experiments
    Volume2016
    Issue number114
    DOIs
    StatePublished - Aug 24 2016

    Profile

    Spinal Ganglia
    Axons
    Neurons
    Edema Disease of Swine
    Antitubercular Agents
    Nitrogenase
    Civil Rights
    Schwann Cells
    Demyelinating Diseases
    Coculture Techniques
    Mesenchymal Stromal Cells
    Regeneration
    Wounds and Injuries
    Erythrasma
    Arachnoiditis
    Status Epilepticus
    Castration
    Cell culture
    Stiffness
    Stem cells

    Keywords

    • Anisotropy
    • Axon alignment
    • DRG isolation
    • Issue 114
    • Myelination
    • Neuroscience
    • Pre-stretch
    • Schwann cell

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Chemical Engineering(all)
    • Immunology and Microbiology(all)
    • Neuroscience(all)

    Cite this

    An approach to enhance alignment and myelination of dorsal root ganglion neurons. / Liu, Chun; Chan, Christina.

    In: Journal of Visualized Experiments, Vol. 2016, No. 114, e54085, 24.08.2016.

    Research output: Contribution to journalArticle

    Liu, Chun; Chan, Christina / An approach to enhance alignment and myelination of dorsal root ganglion neurons.

    In: Journal of Visualized Experiments, Vol. 2016, No. 114, e54085, 24.08.2016.

    Research output: Contribution to journalArticle

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