An electrochemical interface for integrated biosensors

Peter Kim, Neeraj Kohli, Brian Hassler, Nathan Dotson, Andrew Mason, R. Mark Worden, Robert Ofoli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

  • 10 Citations

Abstract

This paper presents an integrated, protein-based, biosensor that can be scaled to form high-density, multi-analyte sensor arrays physically integrated on a signal conditioning circuit die. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors has been developed. A silicon substrate electrode system, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode has been adapted to biomimetic sensors. The functional Ag/AgCl reference electrode is isolated from the environment using a Nafion cation-exchange membrane to extend operation lifetime. To complete the sensor structure, lipid bilayers have been deposited in passivation layer openings formed over individual working electrodes using a special tethering molecule. Total internal reflection microscopy (TIRFM) studies were done to confirm that a wide range of proteins, such as dehydrogenase enzymes and ion channels, can then be embedded into the lipid bilayers. These results verify the potential to form highly selective recognition elements with direct physical connection to readout electronics on the supporting silicon substrate.

LanguageEnglish (US)
Title of host publicationProceedings of IEEE Sensors
Pages1036-1040
Number of pages5
Volume2
Edition2
StatePublished - 2003
EventSecond IEEE International Conference on Sensors: IEEE Sensors 2003 - Toronto, Ont., Canada
Duration: Oct 22 2003Oct 24 2003

Other

OtherSecond IEEE International Conference on Sensors: IEEE Sensors 2003
CountryCanada
CityToronto, Ont.
Period10/22/0310/24/03

Profile

Biosensors
Electrodes
Lipid bilayers
Sensors
Signal conditioning circuits
Proteins
Silicon
Sensor arrays
Biomimetics
Substrates
Passivation
Ion exchange
Microscopic examination
Electronic equipment
Enzymes
Positive ions
Membranes
Molecules
Ions

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Kim, P., Kohli, N., Hassler, B., Dotson, N., Mason, A., Mark Worden, R., & Ofoli, R. (2003). An electrochemical interface for integrated biosensors. In Proceedings of IEEE Sensors (2 ed., Vol. 2, pp. 1036-1040)

An electrochemical interface for integrated biosensors. / Kim, Peter; Kohli, Neeraj; Hassler, Brian; Dotson, Nathan; Mason, Andrew; Mark Worden, R.; Ofoli, Robert.

Proceedings of IEEE Sensors. Vol. 2 2. ed. 2003. p. 1036-1040.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, P, Kohli, N, Hassler, B, Dotson, N, Mason, A, Mark Worden, R & Ofoli, R 2003, An electrochemical interface for integrated biosensors. in Proceedings of IEEE Sensors. 2 edn, vol. 2, pp. 1036-1040, Second IEEE International Conference on Sensors: IEEE Sensors 2003, Toronto, Ont., Canada, 10/22/03.
Kim P, Kohli N, Hassler B, Dotson N, Mason A, Mark Worden R et al. An electrochemical interface for integrated biosensors. In Proceedings of IEEE Sensors. 2 ed. Vol. 2. 2003. p. 1036-1040.
Kim, Peter ; Kohli, Neeraj ; Hassler, Brian ; Dotson, Nathan ; Mason, Andrew ; Mark Worden, R. ; Ofoli, Robert. / An electrochemical interface for integrated biosensors. Proceedings of IEEE Sensors. Vol. 2 2. ed. 2003. pp. 1036-1040
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