An electrochemical interface for integrated biosensors

Peter Kim, Neeraj Kohli, Brian Hassler, Nathan Dotson, Andrew Mason, R. Mark Worden, Robert Ofoli

    Research output: ResearchConference contribution

    • 9 Citations

    Abstract

    This paper presents an integrated, protein-based, biosensor that can be scaled to form high-density, multi-analyte sensor arrays physically integrated on a signal conditioning circuit die. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors has been developed. A silicon substrate electrode system, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode has been adapted to biomimetic sensors. The functional Ag/AgCl reference electrode is isolated from the environment using a Nafion cation-exchange membrane to extend operation lifetime. To complete the sensor structure, lipid bilayers have been deposited in passivation layer openings formed over individual working electrodes using a special tethering molecule. Total internal reflection microscopy (TIRFM) studies were done to confirm that a wide range of proteins, such as dehydrogenase enzymes and ion channels, can then be embedded into the lipid bilayers. These results verify the potential to form highly selective recognition elements with direct physical connection to readout electronics on the supporting silicon substrate.

    LanguageEnglish (US)
    Title of host publicationProceedings of IEEE Sensors
    Pages1036-1040
    Number of pages5
    Volume2
    Edition2
    StatePublished - 2003
    EventSecond IEEE International Conference on Sensors: IEEE Sensors 2003 - Toronto, Ont., Canada
    Duration: Oct 22 2003Oct 24 2003

    Other

    OtherSecond IEEE International Conference on Sensors: IEEE Sensors 2003
    CountryCanada
    CityToronto, Ont.
    Period10/22/0310/24/03

    Profile

    Biosensors
    Electrodes
    Sensors
    Lipid bilayers
    Proteins
    Silicon
    Substrates
    Signal conditioning circuits
    Sensor arrays
    Biomimetics
    Passivation
    Ion exchange
    Microscopic examination
    Electronic equipment
    Enzymes
    Positive ions
    Membranes
    Molecules
    Ions
    Oxidoreductases

    ASJC Scopus subject areas

    • Engineering (miscellaneous)
    • Electrical and Electronic Engineering

    Cite this

    Kim, P., Kohli, N., Hassler, B., Dotson, N., Mason, A., Mark Worden, R., & Ofoli, R. (2003). An electrochemical interface for integrated biosensors. In Proceedings of IEEE Sensors (2 ed., Vol. 2, pp. 1036-1040)

    An electrochemical interface for integrated biosensors. / Kim, Peter; Kohli, Neeraj; Hassler, Brian; Dotson, Nathan; Mason, Andrew; Mark Worden, R.; Ofoli, Robert.

    Proceedings of IEEE Sensors. Vol. 2 2. ed. 2003. p. 1036-1040.

    Research output: ResearchConference contribution

    Kim, P, Kohli, N, Hassler, B, Dotson, N, Mason, A, Mark Worden, R & Ofoli, R 2003, An electrochemical interface for integrated biosensors. in Proceedings of IEEE Sensors. 2 edn, vol. 2, pp. 1036-1040, Second IEEE International Conference on Sensors: IEEE Sensors 2003, Toronto, Ont., Canada, 10/22/03.
    Kim P, Kohli N, Hassler B, Dotson N, Mason A, Mark Worden R et al. An electrochemical interface for integrated biosensors. In Proceedings of IEEE Sensors. 2 ed. Vol. 2. 2003. p. 1036-1040.
    Kim, Peter ; Kohli, Neeraj ; Hassler, Brian ; Dotson, Nathan ; Mason, Andrew ; Mark Worden, R. ; Ofoli, Robert. / An electrochemical interface for integrated biosensors. Proceedings of IEEE Sensors. Vol. 2 2. ed. 2003. pp. 1036-1040
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