Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI

Erik M. Shapiro, Laura N. Medford-Davis, Tarek M. Fahmy, Cynthia E. Dunbar, Alan P. Koretsky

Research output: Contribution to journalArticle

  • 48 Citations

Abstract

Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, in vitro, with streptavidin-coated micron-sized iron oxide particles (MPIOs), achieving iron concentrations as high as 60 pg iron per cell. This is 30 times the amount of labeling reported with ultrasmall particles of iron oxide (USPIOs). Labeling was mediated by use of a biotinylated anti-CD5 antibody, which is specific for peripheral T cells. Such labeling allowed the in vitro detection of single lymphocytes by MRI, using conditions well suited for in vivo animal work. Electron microscopic analysis demonstrated that MPIOs remained largely extracellular after labeling, with some evidence of intracellular uptake. Cell viability and early and late cytokine release studies showed no significant differences between labeled and unlabeled cells. Therefore, the use of MPIOs for achieving high iron concentrations for cellular MRI is potentially an effective new modality for non-invasive imaging of lymphocytes.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalContrast Media and Molecular Imaging
Volume2
Issue number3
DOIs
StatePublished - May 2007
Externally publishedYes

Profile

Oxides
Iron
T-Lymphocytes
Antibodies
Lymphocytes
In Vitro Techniques
Streptavidin
Anti-Idiotypic Antibodies
Cell Survival
Electrons
Cytokines

Keywords

  • Contrast agents
  • Iron oxide
  • MRI
  • T cells

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI. / Shapiro, Erik M.; Medford-Davis, Laura N.; Fahmy, Tarek M.; Dunbar, Cynthia E.; Koretsky, Alan P.

In: Contrast Media and Molecular Imaging, Vol. 2, No. 3, 05.2007, p. 147-153.

Research output: Contribution to journalArticle

Shapiro, Erik M.; Medford-Davis, Laura N.; Fahmy, Tarek M.; Dunbar, Cynthia E.; Koretsky, Alan P. / Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI.

In: Contrast Media and Molecular Imaging, Vol. 2, No. 3, 05.2007, p. 147-153.

Research output: Contribution to journalArticle

@article{e855fe823bff40f1b982bc5e9c01f829,
title = "Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI",
abstract = "Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, in vitro, with streptavidin-coated micron-sized iron oxide particles (MPIOs), achieving iron concentrations as high as 60 pg iron per cell. This is 30 times the amount of labeling reported with ultrasmall particles of iron oxide (USPIOs). Labeling was mediated by use of a biotinylated anti-CD5 antibody, which is specific for peripheral T cells. Such labeling allowed the in vitro detection of single lymphocytes by MRI, using conditions well suited for in vivo animal work. Electron microscopic analysis demonstrated that MPIOs remained largely extracellular after labeling, with some evidence of intracellular uptake. Cell viability and early and late cytokine release studies showed no significant differences between labeled and unlabeled cells. Therefore, the use of MPIOs for achieving high iron concentrations for cellular MRI is potentially an effective new modality for non-invasive imaging of lymphocytes.",
keywords = "Contrast agents, Iron oxide, MRI, T cells",
author = "Shapiro, {Erik M.} and Medford-Davis, {Laura N.} and Fahmy, {Tarek M.} and Dunbar, {Cynthia E.} and Koretsky, {Alan P.}",
year = "2007",
month = "5",
doi = "10.1002/cmmi.134",
volume = "2",
pages = "147--153",
journal = "Contrast Media and Molecular Imaging",
issn = "1555-4309",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI

AU - Shapiro,Erik M.

AU - Medford-Davis,Laura N.

AU - Fahmy,Tarek M.

AU - Dunbar,Cynthia E.

AU - Koretsky,Alan P.

PY - 2007/5

Y1 - 2007/5

N2 - Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, in vitro, with streptavidin-coated micron-sized iron oxide particles (MPIOs), achieving iron concentrations as high as 60 pg iron per cell. This is 30 times the amount of labeling reported with ultrasmall particles of iron oxide (USPIOs). Labeling was mediated by use of a biotinylated anti-CD5 antibody, which is specific for peripheral T cells. Such labeling allowed the in vitro detection of single lymphocytes by MRI, using conditions well suited for in vivo animal work. Electron microscopic analysis demonstrated that MPIOs remained largely extracellular after labeling, with some evidence of intracellular uptake. Cell viability and early and late cytokine release studies showed no significant differences between labeled and unlabeled cells. Therefore, the use of MPIOs for achieving high iron concentrations for cellular MRI is potentially an effective new modality for non-invasive imaging of lymphocytes.

AB - Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, in vitro, with streptavidin-coated micron-sized iron oxide particles (MPIOs), achieving iron concentrations as high as 60 pg iron per cell. This is 30 times the amount of labeling reported with ultrasmall particles of iron oxide (USPIOs). Labeling was mediated by use of a biotinylated anti-CD5 antibody, which is specific for peripheral T cells. Such labeling allowed the in vitro detection of single lymphocytes by MRI, using conditions well suited for in vivo animal work. Electron microscopic analysis demonstrated that MPIOs remained largely extracellular after labeling, with some evidence of intracellular uptake. Cell viability and early and late cytokine release studies showed no significant differences between labeled and unlabeled cells. Therefore, the use of MPIOs for achieving high iron concentrations for cellular MRI is potentially an effective new modality for non-invasive imaging of lymphocytes.

KW - Contrast agents

KW - Iron oxide

KW - MRI

KW - T cells

UR - http://www.scopus.com/inward/record.url?scp=34547540488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547540488&partnerID=8YFLogxK

U2 - 10.1002/cmmi.134

DO - 10.1002/cmmi.134

M3 - Article

VL - 2

SP - 147

EP - 153

JO - Contrast Media and Molecular Imaging

T2 - Contrast Media and Molecular Imaging

JF - Contrast Media and Molecular Imaging

SN - 1555-4309

IS - 3

ER -