Application and analysis of structure-switching aptamers for small molecule quantification

Shengnan Xie, S. Patrick Walton

Research output: Contribution to journalArticle

  • 6 Citations

Abstract

Modern tools for the analysis of cellular function aim for the quantitative measurement of all members of a given class of biological molecules. Of the analyte classes, nucleic acid measurements are typically the most tractable, both on an individual analyte basis and in parallel. Thus, tools are being sought to enable measurement of other cellular molecules using nucleic acid biosensors. Of the variety of potential nucleic acid biosensor strategies, structure-switching aptamers (SSAs) present a unique opportunity to couple sensing and readout of the target molecule. However, little has been characterized about the parameters that determine the fidelity of the signal from SSA biosensors. In this study, a small molecule biosensor based on a SSA was engineered to detect the model small molecule, theophylline, in solution. Quantitative theophylline detection over nearly three orders-of-magnitude was achieved by scintillation counting and quantitative PCR. Further analysis showed that the biosensor fidelity is primarily controlled by the relative stability of the two conformations of the SSA.

LanguageEnglish (US)
Pages213-219
Number of pages7
JournalAnalytica Chimica Acta
Volume638
Issue number2
DOIs
StatePublished - Apr 13 2009

Profile

Biosensing Techniques
nucleic acid
Biosensors
Molecules
Nucleic Acids
Theophylline
Scintillation Counting
Scintillation
Conformations
analysis
Polymerase Chain Reaction

Keywords

  • Affinity
  • Aptamer
  • Biosensor design
  • Molecular barcode
  • Structure switching
  • Theophylline

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Application and analysis of structure-switching aptamers for small molecule quantification. / Xie, Shengnan; Walton, S. Patrick.

In: Analytica Chimica Acta, Vol. 638, No. 2, 13.04.2009, p. 213-219.

Research output: Contribution to journalArticle

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