Application and analysis of structure-switching aptamers for small molecule quantification

Shengnan Xie, S. Patrick Walton

    Research output: Contribution to journalArticle

    • 6 Citations

    Abstract

    Modern tools for the analysis of cellular function aim for the quantitative measurement of all members of a given class of biological molecules. Of the analyte classes, nucleic acid measurements are typically the most tractable, both on an individual analyte basis and in parallel. Thus, tools are being sought to enable measurement of other cellular molecules using nucleic acid biosensors. Of the variety of potential nucleic acid biosensor strategies, structure-switching aptamers (SSAs) present a unique opportunity to couple sensing and readout of the target molecule. However, little has been characterized about the parameters that determine the fidelity of the signal from SSA biosensors. In this study, a small molecule biosensor based on a SSA was engineered to detect the model small molecule, theophylline, in solution. Quantitative theophylline detection over nearly three orders-of-magnitude was achieved by scintillation counting and quantitative PCR. Further analysis showed that the biosensor fidelity is primarily controlled by the relative stability of the two conformations of the SSA.

    Original languageEnglish (US)
    Pages (from-to)213-219
    Number of pages7
    JournalAnalytica Chimica Acta
    Volume638
    Issue number2
    DOIs
    StatePublished - Apr 13 2009

    Profile

    Molecules
    Biosensing Techniques
    nucleic acid
    Bicyclo Compounds
    Biosensors
    Nucleic Acids
    Buccal Administration
    Theophylline
    Scintillation Counting
    Polymerase Chain Reaction
    Bibliography of Medicine
    Birth Injuries
    Anthralin
    Bacillus

    Keywords

    • Affinity
    • Aptamer
    • Biosensor design
    • Molecular barcode
    • Structure switching
    • Theophylline

    ASJC Scopus subject areas

    • Biochemistry
    • Analytical Chemistry
    • Spectroscopy
    • Environmental Chemistry

    Cite this

    Application and analysis of structure-switching aptamers for small molecule quantification. / Xie, Shengnan; Walton, S. Patrick.

    In: Analytica Chimica Acta, Vol. 638, No. 2, 13.04.2009, p. 213-219.

    Research output: Contribution to journalArticle

    Xie, Shengnan; Walton, S. Patrick / Application and analysis of structure-switching aptamers for small molecule quantification.

    In: Analytica Chimica Acta, Vol. 638, No. 2, 13.04.2009, p. 213-219.

    Research output: Contribution to journalArticle

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