Application of metabolic engineering to cellular processes of the liver: Bioartificial liver development

Christina Chan, Junji Washizu, Francois Berthiaume, Mehmet Toner, Martin L. Yarmush

Research output: ResearchConference contribution

Abstract

The effect of insulin pre-conditioning and amino acid supplementation on the accumulation of lipids was investigated. Hepatocytes were cultured in the collagen sandwich configuration in culture medium for six days prior to exposing the cells to plasma to allow recovery from the isolation. Metabolic flux analysis (MFA) was used to quantify the changes in lipogenic fluxes and pathways within the cells.

LanguageEnglish (US)
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
EditorsR.D. Kamm, G.W. Schmid-Schoenbein, G.A. Atesian, M.S. Hefzy
Pages357-358
Number of pages2
Volume50
StatePublished - 2001
Externally publishedYes
EventProceedings of the 2001 Bioengineering Conference - Snowbird, UT, United States
Duration: Jun 27 2001Jul 1 2001

Other

OtherProceedings of the 2001 Bioengineering Conference
CountryUnited States
CitySnowbird, UT
Period6/27/017/1/01

Profile

Metabolic engineering
Liver
Fluxes
Insulin
Collagen
Lipids
Amino acids
Plasmas
Recovery
Hepatocytes

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Chan, C., Washizu, J., Berthiaume, F., Toner, M., & Yarmush, M. L. (2001). Application of metabolic engineering to cellular processes of the liver: Bioartificial liver development. In R. D. Kamm, G. W. Schmid-Schoenbein, G. A. Atesian, & M. S. Hefzy (Eds.), American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (Vol. 50, pp. 357-358)

Application of metabolic engineering to cellular processes of the liver : Bioartificial liver development. / Chan, Christina; Washizu, Junji; Berthiaume, Francois; Toner, Mehmet; Yarmush, Martin L.

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. ed. / R.D. Kamm; G.W. Schmid-Schoenbein; G.A. Atesian; M.S. Hefzy. Vol. 50 2001. p. 357-358.

Research output: ResearchConference contribution

Chan, C, Washizu, J, Berthiaume, F, Toner, M & Yarmush, ML 2001, Application of metabolic engineering to cellular processes of the liver: Bioartificial liver development. in RD Kamm, GW Schmid-Schoenbein, GA Atesian & MS Hefzy (eds), American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. vol. 50, pp. 357-358, Proceedings of the 2001 Bioengineering Conference, Snowbird, UT, United States, 6/27/01.
Chan C, Washizu J, Berthiaume F, Toner M, Yarmush ML. Application of metabolic engineering to cellular processes of the liver: Bioartificial liver development. In Kamm RD, Schmid-Schoenbein GW, Atesian GA, Hefzy MS, editors, American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 50. 2001. p. 357-358.
Chan, Christina ; Washizu, Junji ; Berthiaume, Francois ; Toner, Mehmet ; Yarmush, Martin L./ Application of metabolic engineering to cellular processes of the liver : Bioartificial liver development. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. editor / R.D. Kamm ; G.W. Schmid-Schoenbein ; G.A. Atesian ; M.S. Hefzy. Vol. 50 2001. pp. 357-358
@inbook{51b651e7a02246358a6cd316cb955708,
title = "Application of metabolic engineering to cellular processes of the liver: Bioartificial liver development",
abstract = "The effect of insulin pre-conditioning and amino acid supplementation on the accumulation of lipids was investigated. Hepatocytes were cultured in the collagen sandwich configuration in culture medium for six days prior to exposing the cells to plasma to allow recovery from the isolation. Metabolic flux analysis (MFA) was used to quantify the changes in lipogenic fluxes and pathways within the cells.",
author = "Christina Chan and Junji Washizu and Francois Berthiaume and Mehmet Toner and Yarmush, {Martin L.}",
year = "2001",
volume = "50",
pages = "357--358",
editor = "R.D. Kamm and G.W. Schmid-Schoenbein and G.A. Atesian and M.S. Hefzy",
booktitle = "American Society of Mechanical Engineers, Bioengineering Division (Publication) BED",

}

TY - CHAP

T1 - Application of metabolic engineering to cellular processes of the liver

T2 - Bioartificial liver development

AU - Chan,Christina

AU - Washizu,Junji

AU - Berthiaume,Francois

AU - Toner,Mehmet

AU - Yarmush,Martin L.

PY - 2001

Y1 - 2001

N2 - The effect of insulin pre-conditioning and amino acid supplementation on the accumulation of lipids was investigated. Hepatocytes were cultured in the collagen sandwich configuration in culture medium for six days prior to exposing the cells to plasma to allow recovery from the isolation. Metabolic flux analysis (MFA) was used to quantify the changes in lipogenic fluxes and pathways within the cells.

AB - The effect of insulin pre-conditioning and amino acid supplementation on the accumulation of lipids was investigated. Hepatocytes were cultured in the collagen sandwich configuration in culture medium for six days prior to exposing the cells to plasma to allow recovery from the isolation. Metabolic flux analysis (MFA) was used to quantify the changes in lipogenic fluxes and pathways within the cells.

UR - http://www.scopus.com/inward/record.url?scp=0242694016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242694016&partnerID=8YFLogxK

M3 - Conference contribution

VL - 50

SP - 357

EP - 358

BT - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

ER -