Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Neeraj Kohli, Sachin Vaidya, Robert Y. Ofoli, Robert M. Worden, Ilsoon Lee

    Research output: Contribution to journalArticle

    • 24 Citations

    Abstract

    This paper presents novel methods to produce arrays of lipid bilayers and liposomes on patterned polyelectrolyte multilayers. We created the arrays by exposing patterns of poly(dimethyldiallylammonium chloride) (PDAC), polyethylene glycol (m-dPEG) acid, and poly(allylamine hydrochloride) (PAH) on polyelectrolyte multilayers (PEMs) to liposomes of various compositions. The resulting interfaces were characterized by total internal reflection fluorescence microscopy (TIRFM), fluorescence recovery after pattern photobleaching (FRAPP), quartz crystal microbalance (QCM), and fluorescence microscopy. Liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (monosodium salt) (DOPA) were found to preferentially adsorb on PDAC and PAH surfaces. On the other hand, liposome adsorption on sulfonated poly(styrene) (SPS) surfaces was minimal, due to electrostatic repulsion between the negatively charged liposomes and the SPS-coated surface. Surfaces coated with m-dPEG acid were also found to resist liposome adsorption. We exploited these results to create arrays of lipid bilayers by exposing PDAC, PAH and m-dPEG patterned substrates to DOPA/DOPC vesicles of various compositions. The patterned substrates were created by stamping PDAC (or PAH) on SPS-topped multilayers, and m-dPEG acid on PDAC-topped multilayers, respectively. This technique can be used to produce functional biomimetic interfaces for potential applications in biosensors and biocatalysis, for creating arrays that could be used for high-throughput screening of compounds that interact with cell membranes, and for probing, and possibly controlling, interactions between living cells and synthetic membranes.

    Original languageEnglish (US)
    Pages (from-to)461-469
    Number of pages9
    JournalJournal of Colloid and Interface Science
    Volume301
    Issue number2
    DOIs
    StatePublished - Sep 15 2006

    Profile

    chlorides
    Liposomes
    polycyclic aromatic hydrocarbons
    lipids
    fluorescence
    acids
    Polycyclic aromatic hydrocarbons
    Multilayers
    Edema Disease of Swine
    Bacillaceae
    Micelles
    Lipid bilayers
    Polyelectrolytes
    Acids
    Magnesium Deficiency
    microscopy
    adsorption
    Fluorescence microscopy
    Adsorption
    Substrates

    Keywords

    • BLM
    • Fluorescence microscopy
    • Fluorescence recovery after pattern photobleaching (FRAPP)
    • Lipid bilayers
    • Liposomes
    • Microarray
    • Polyelectrolytes
    • Quartz crystal microbalance (QCM)
    • Total internal reflection fluorescence microscopy (TIRFM)

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates. / Kohli, Neeraj; Vaidya, Sachin; Ofoli, Robert Y.; Worden, Robert M.; Lee, Ilsoon.

    In: Journal of Colloid and Interface Science, Vol. 301, No. 2, 15.09.2006, p. 461-469.

    Research output: Contribution to journalArticle

    Kohli, Neeraj; Vaidya, Sachin; Ofoli, Robert Y.; Worden, Robert M.; Lee, Ilsoon / Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates.

    In: Journal of Colloid and Interface Science, Vol. 301, No. 2, 15.09.2006, p. 461-469.

    Research output: Contribution to journalArticle

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    abstract = "This paper presents novel methods to produce arrays of lipid bilayers and liposomes on patterned polyelectrolyte multilayers. We created the arrays by exposing patterns of poly(dimethyldiallylammonium chloride) (PDAC), polyethylene glycol (m-dPEG) acid, and poly(allylamine hydrochloride) (PAH) on polyelectrolyte multilayers (PEMs) to liposomes of various compositions. The resulting interfaces were characterized by total internal reflection fluorescence microscopy (TIRFM), fluorescence recovery after pattern photobleaching (FRAPP), quartz crystal microbalance (QCM), and fluorescence microscopy. Liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (monosodium salt) (DOPA) were found to preferentially adsorb on PDAC and PAH surfaces. On the other hand, liposome adsorption on sulfonated poly(styrene) (SPS) surfaces was minimal, due to electrostatic repulsion between the negatively charged liposomes and the SPS-coated surface. Surfaces coated with m-dPEG acid were also found to resist liposome adsorption. We exploited these results to create arrays of lipid bilayers by exposing PDAC, PAH and m-dPEG patterned substrates to DOPA/DOPC vesicles of various compositions. The patterned substrates were created by stamping PDAC (or PAH) on SPS-topped multilayers, and m-dPEG acid on PDAC-topped multilayers, respectively. This technique can be used to produce functional biomimetic interfaces for potential applications in biosensors and biocatalysis, for creating arrays that could be used for high-throughput screening of compounds that interact with cell membranes, and for probing, and possibly controlling, interactions between living cells and synthetic membranes.",
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    T1 - Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

    AU - Kohli,Neeraj

    AU - Vaidya,Sachin

    AU - Ofoli,Robert Y.

    AU - Worden,Robert M.

    AU - Lee,Ilsoon

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    N2 - This paper presents novel methods to produce arrays of lipid bilayers and liposomes on patterned polyelectrolyte multilayers. We created the arrays by exposing patterns of poly(dimethyldiallylammonium chloride) (PDAC), polyethylene glycol (m-dPEG) acid, and poly(allylamine hydrochloride) (PAH) on polyelectrolyte multilayers (PEMs) to liposomes of various compositions. The resulting interfaces were characterized by total internal reflection fluorescence microscopy (TIRFM), fluorescence recovery after pattern photobleaching (FRAPP), quartz crystal microbalance (QCM), and fluorescence microscopy. Liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (monosodium salt) (DOPA) were found to preferentially adsorb on PDAC and PAH surfaces. On the other hand, liposome adsorption on sulfonated poly(styrene) (SPS) surfaces was minimal, due to electrostatic repulsion between the negatively charged liposomes and the SPS-coated surface. Surfaces coated with m-dPEG acid were also found to resist liposome adsorption. We exploited these results to create arrays of lipid bilayers by exposing PDAC, PAH and m-dPEG patterned substrates to DOPA/DOPC vesicles of various compositions. The patterned substrates were created by stamping PDAC (or PAH) on SPS-topped multilayers, and m-dPEG acid on PDAC-topped multilayers, respectively. This technique can be used to produce functional biomimetic interfaces for potential applications in biosensors and biocatalysis, for creating arrays that could be used for high-throughput screening of compounds that interact with cell membranes, and for probing, and possibly controlling, interactions between living cells and synthetic membranes.

    AB - This paper presents novel methods to produce arrays of lipid bilayers and liposomes on patterned polyelectrolyte multilayers. We created the arrays by exposing patterns of poly(dimethyldiallylammonium chloride) (PDAC), polyethylene glycol (m-dPEG) acid, and poly(allylamine hydrochloride) (PAH) on polyelectrolyte multilayers (PEMs) to liposomes of various compositions. The resulting interfaces were characterized by total internal reflection fluorescence microscopy (TIRFM), fluorescence recovery after pattern photobleaching (FRAPP), quartz crystal microbalance (QCM), and fluorescence microscopy. Liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (monosodium salt) (DOPA) were found to preferentially adsorb on PDAC and PAH surfaces. On the other hand, liposome adsorption on sulfonated poly(styrene) (SPS) surfaces was minimal, due to electrostatic repulsion between the negatively charged liposomes and the SPS-coated surface. Surfaces coated with m-dPEG acid were also found to resist liposome adsorption. We exploited these results to create arrays of lipid bilayers by exposing PDAC, PAH and m-dPEG patterned substrates to DOPA/DOPC vesicles of various compositions. The patterned substrates were created by stamping PDAC (or PAH) on SPS-topped multilayers, and m-dPEG acid on PDAC-topped multilayers, respectively. This technique can be used to produce functional biomimetic interfaces for potential applications in biosensors and biocatalysis, for creating arrays that could be used for high-throughput screening of compounds that interact with cell membranes, and for probing, and possibly controlling, interactions between living cells and synthetic membranes.

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    KW - Fluorescence microscopy

    KW - Fluorescence recovery after pattern photobleaching (FRAPP)

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    KW - Liposomes

    KW - Microarray

    KW - Polyelectrolytes

    KW - Quartz crystal microbalance (QCM)

    KW - Total internal reflection fluorescence microscopy (TIRFM)

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