Assessment of bacterial and fungal (hemi)cellulose-degrading enzymes in saccharification of ammonia fibre expansion-pretreated Arundo donax

Simona Giacobbe, Venkatesh Balan, Salvatore Montella, Massimo Fagnano, Mauro Mori, Vincenza Faraco

Research output: Contribution to journalArticle

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Abstract

This study reports enzymatic hydrolysis of the biomass of the giant reed (Arundo donax L.) after ammonia fibre expansion (AFEX) pretreatment. In particular, the capacity of the arabinofuranosidase from the fungus Pleurotus ostreatus recombinantly expressed in Pichia pastoris rPoAbf, its evolved mutant rPoAbf F435Y/Y446F and the endo-cellulase from Streptomyces sp. G12 CelStrep recombinantly expressed in Escherichia coli to enhance the hydrolysis of AFEX-treated A. donax was investigated, using the corn stover as reference feedstock. The investigated enzymes were assayed using a mixture of purified cellulases (CBHI, CBHII, EGI and βG), endoxylanases (LX3, LX4) and accessory hemicellulases (LarbF and LβX) as reference enzyme mixture and substituting EGI with rCelStrep and LarbF with rPoAbf or rPoAbf F435Y/Y446F. The use of rPoAbf F435Y/Y446F in the substitution of LarbF led to improvements in sugar conversion, giving a glucan, xylan and arabinan conversion after 72 h of around 62, 63 and 80 %, respectively, similar or higher than those (44, 66 and 55 %) achieved by 72 h hydrolysis with commercial enzymes Novozymes Cellic®, Ctec3 and Htec3. The enzymes rPoAbf, rPoAbf F435Y/Y446F and rCelStrep were also investigated for their effect on hydrolysis of AFEX-pretreated A. donax by addition to commercial enzyme mixture Novozymes Cellic®, Ctec3 and Htec3, and it was shown that the addition of rPoAbf and its evolved mutant rPoAbf F435Y/Y446F enhanced both xylan and arabinan conversions, which achieved 80 % after 6 days of saccharification with rPoAbf F435Y/Y446F.

LanguageEnglish (US)
Pages2213-2224
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume100
Issue number5
DOIs
StatePublished - Mar 1 2016

Profile

Poaceae
Ammonia
Cellulose
Hydrolysis
Enzymes
Xylans
Endo-1,4-beta Xylanases
Pleurotus
Cellulases
Pichia
Glucans
Cellulase
Streptomyces
Biomass
Zea mays
Fungi
Escherichia coli
araban

Keywords

  • Arabinofuranosidase
  • Cellulase
  • Lignocellulose
  • Pretreatment

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Assessment of bacterial and fungal (hemi)cellulose-degrading enzymes in saccharification of ammonia fibre expansion-pretreated Arundo donax. / Giacobbe, Simona; Balan, Venkatesh; Montella, Salvatore; Fagnano, Massimo; Mori, Mauro; Faraco, Vincenza.

In: Applied Microbiology and Biotechnology, Vol. 100, No. 5, 01.03.2016, p. 2213-2224.

Research output: Contribution to journalArticle

Giacobbe, Simona ; Balan, Venkatesh ; Montella, Salvatore ; Fagnano, Massimo ; Mori, Mauro ; Faraco, Vincenza. / Assessment of bacterial and fungal (hemi)cellulose-degrading enzymes in saccharification of ammonia fibre expansion-pretreated Arundo donax. In: Applied Microbiology and Biotechnology. 2016 ; Vol. 100, No. 5. pp. 2213-2224
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