Augmenting protein release from layer-by-layer functionalized agarose hydrogels

Daniel Lynam, Chelsea Peterson, Ryan Maloney, Dena Shahriari, Alexa Garrison, Sara Saleh, Sumit Mehrotra, Christina Chan, Jeff Sakamoto

    Research output: Research - peer-reviewArticle

    • 7 Citations

    Abstract

    Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface area per unit volume of hydrogel. The surface area of a range of compositions (1.5-5.0 wt% agarose and 0, 50 and 65 wt% sucrose) was measured. Gels were supercritically dried to preserve porosity enabling detailed pore morphology measurements using nitrogen adsorption and high resolution scanning electron microscopy. The resulting surface area, normalized by superficial gel volume, ranged between 6 m2/cm3gel and 56 m2/cm3gel. Using the layer-by-layer process to load lysozyme, a neurotrophic factor analog, a relationship was observed between surface area and cumulative dose response ranging from 176 to 2556 μg/mL, which is in the range of clinical relevance for the delivery of growth factors. In this work, we demonstrated that the ability to control porosity is key in tuning drug delivery dose response from layer-by-layer modified hydrogels.

    LanguageEnglish (US)
    Pages377-384
    Number of pages8
    JournalCarbohydrate Polymers
    Volume103
    Issue number1
    DOIs
    StatePublished - Mar 15 2014

    Profile

    Hydrogels
    Sepharose
    Proteins
    Gels
    Hydrogel
    Sucrose
    Porosity
    Sugar (sucrose)
    Nanopores
    High resolution electron microscopy
    Nerve Growth Factors
    Gelation
    Muramidase
    Drug delivery
    Scaffolds
    Intercellular Signaling Peptides and Proteins
    Repair
    Nitrogen
    Tuning
    Adsorption

    Keywords

    • Controlled drug release
    • Hydrogel
    • Layer-by-layer
    • Pore refinement
    • Surface area

    ASJC Scopus subject areas

    • Organic Chemistry
    • Materials Chemistry
    • Polymers and Plastics

    Cite this

    Lynam, D., Peterson, C., Maloney, R., Shahriari, D., Garrison, A., Saleh, S., ... Sakamoto, J. (2014). Augmenting protein release from layer-by-layer functionalized agarose hydrogels. Carbohydrate Polymers, 103(1), 377-384. DOI: 10.1016/j.carbpol.2013.12.069

    Augmenting protein release from layer-by-layer functionalized agarose hydrogels. / Lynam, Daniel; Peterson, Chelsea; Maloney, Ryan; Shahriari, Dena; Garrison, Alexa; Saleh, Sara; Mehrotra, Sumit; Chan, Christina; Sakamoto, Jeff.

    In: Carbohydrate Polymers, Vol. 103, No. 1, 15.03.2014, p. 377-384.

    Research output: Research - peer-reviewArticle

    Lynam, D, Peterson, C, Maloney, R, Shahriari, D, Garrison, A, Saleh, S, Mehrotra, S, Chan, C & Sakamoto, J 2014, 'Augmenting protein release from layer-by-layer functionalized agarose hydrogels' Carbohydrate Polymers, vol 103, no. 1, pp. 377-384. DOI: 10.1016/j.carbpol.2013.12.069
    Lynam D, Peterson C, Maloney R, Shahriari D, Garrison A, Saleh S et al. Augmenting protein release from layer-by-layer functionalized agarose hydrogels. Carbohydrate Polymers. 2014 Mar 15;103(1):377-384. Available from, DOI: 10.1016/j.carbpol.2013.12.069
    Lynam, Daniel ; Peterson, Chelsea ; Maloney, Ryan ; Shahriari, Dena ; Garrison, Alexa ; Saleh, Sara ; Mehrotra, Sumit ; Chan, Christina ; Sakamoto, Jeff. / Augmenting protein release from layer-by-layer functionalized agarose hydrogels. In: Carbohydrate Polymers. 2014 ; Vol. 103, No. 1. pp. 377-384
    @article{f657b189e04441f9921f41acf4a2827c,
    title = "Augmenting protein release from layer-by-layer functionalized agarose hydrogels",
    abstract = "Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface area per unit volume of hydrogel. The surface area of a range of compositions (1.5-5.0 wt% agarose and 0, 50 and 65 wt% sucrose) was measured. Gels were supercritically dried to preserve porosity enabling detailed pore morphology measurements using nitrogen adsorption and high resolution scanning electron microscopy. The resulting surface area, normalized by superficial gel volume, ranged between 6 m2/cm3gel and 56 m2/cm3gel. Using the layer-by-layer process to load lysozyme, a neurotrophic factor analog, a relationship was observed between surface area and cumulative dose response ranging from 176 to 2556 μg/mL, which is in the range of clinical relevance for the delivery of growth factors. In this work, we demonstrated that the ability to control porosity is key in tuning drug delivery dose response from layer-by-layer modified hydrogels.",
    keywords = "Controlled drug release, Hydrogel, Layer-by-layer, Pore refinement, Surface area",
    author = "Daniel Lynam and Chelsea Peterson and Ryan Maloney and Dena Shahriari and Alexa Garrison and Sara Saleh and Sumit Mehrotra and Christina Chan and Jeff Sakamoto",
    year = "2014",
    month = "3",
    doi = "10.1016/j.carbpol.2013.12.069",
    volume = "103",
    pages = "377--384",
    journal = "Carbohydrate Polymers",
    issn = "0144-8617",
    publisher = "Elsevier Limited",
    number = "1",

    }

    TY - JOUR

    T1 - Augmenting protein release from layer-by-layer functionalized agarose hydrogels

    AU - Lynam,Daniel

    AU - Peterson,Chelsea

    AU - Maloney,Ryan

    AU - Shahriari,Dena

    AU - Garrison,Alexa

    AU - Saleh,Sara

    AU - Mehrotra,Sumit

    AU - Chan,Christina

    AU - Sakamoto,Jeff

    PY - 2014/3/15

    Y1 - 2014/3/15

    N2 - Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface area per unit volume of hydrogel. The surface area of a range of compositions (1.5-5.0 wt% agarose and 0, 50 and 65 wt% sucrose) was measured. Gels were supercritically dried to preserve porosity enabling detailed pore morphology measurements using nitrogen adsorption and high resolution scanning electron microscopy. The resulting surface area, normalized by superficial gel volume, ranged between 6 m2/cm3gel and 56 m2/cm3gel. Using the layer-by-layer process to load lysozyme, a neurotrophic factor analog, a relationship was observed between surface area and cumulative dose response ranging from 176 to 2556 μg/mL, which is in the range of clinical relevance for the delivery of growth factors. In this work, we demonstrated that the ability to control porosity is key in tuning drug delivery dose response from layer-by-layer modified hydrogels.

    AB - Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface area per unit volume of hydrogel. The surface area of a range of compositions (1.5-5.0 wt% agarose and 0, 50 and 65 wt% sucrose) was measured. Gels were supercritically dried to preserve porosity enabling detailed pore morphology measurements using nitrogen adsorption and high resolution scanning electron microscopy. The resulting surface area, normalized by superficial gel volume, ranged between 6 m2/cm3gel and 56 m2/cm3gel. Using the layer-by-layer process to load lysozyme, a neurotrophic factor analog, a relationship was observed between surface area and cumulative dose response ranging from 176 to 2556 μg/mL, which is in the range of clinical relevance for the delivery of growth factors. In this work, we demonstrated that the ability to control porosity is key in tuning drug delivery dose response from layer-by-layer modified hydrogels.

    KW - Controlled drug release

    KW - Hydrogel

    KW - Layer-by-layer

    KW - Pore refinement

    KW - Surface area

    UR - http://www.scopus.com/inward/record.url?scp=84892620124&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84892620124&partnerID=8YFLogxK

    U2 - 10.1016/j.carbpol.2013.12.069

    DO - 10.1016/j.carbpol.2013.12.069

    M3 - Article

    VL - 103

    SP - 377

    EP - 384

    JO - Carbohydrate Polymers

    T2 - Carbohydrate Polymers

    JF - Carbohydrate Polymers

    SN - 0144-8617

    IS - 1

    ER -