Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor

Rasika L. Kudahettige-Nilsson, Jonas Helmerius, Robert T. Nilsson, Magnus Sjöblom, David B. Hodge, Ulrika Rova

Research output: Contribution to journalArticle

  • 26 Citations

Abstract

Acetone-butanol-ethanol (ABE) fermentation was studied using acid-hydrolyzed xylan recovered from hardwood Kraft black liquor by CO2 acidification as the only carbon source. Detoxification of hydrolyzate using activated carbon was conducted to evaluate the impact of inhibitor removal and fermentation. Xylose hydrolysis yields as high as 18.4% were demonstrated at the highest severity hydrolysis condition. Detoxification using active carbon was effective for removal of both phenolics (76-81%) and HMF (38-52%). Batch fermentation of the hydrolyzate and semi-defined P2 media resulted in a total solvent yield of 0.12-0.13g/g and 0.34g/g, corresponding to a butanol concentration of 1.8-2.1g/L and 7.3g/L respectively. This work is the first study of a process for the production of a biologically-derived biofuel from hemicelluloses solubilized during Kraft pulping and demonstrates the feasibility of utilizing xylan recovered directly from industrial Kraft pulping liquors as a feedstock for biological production of biofuels such as butanol.

LanguageEnglish (US)
Pages71-79
Number of pages9
JournalBioresource Technology
Volume176
DOIs
StatePublished - Jan 1 2015

Profile

Clostridium acetobutylicum
Betula
Clostridium
Butanols
Xylose
Butenes
Fermentation
fermentation
Kraft process
Xylans
Detoxification
Carbon
Biofuels
detoxification
biofuel
hydrolysis
Hydrolysis
biological production
Acidification
Hardwoods

Keywords

  • ABE fermentation
  • Birch wood kraft black liquor
  • Detoxification
  • Fermentation inhibitors
  • Lignin precipitation

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal
  • Medicine(all)

Cite this

Kudahettige-Nilsson, R. L., Helmerius, J., Nilsson, R. T., Sjöblom, M., Hodge, D. B., & Rova, U. (2015). Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. Bioresource Technology, 176, 71-79. DOI: 10.1016/j.biortech.2014.11.012

Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. / Kudahettige-Nilsson, Rasika L.; Helmerius, Jonas; Nilsson, Robert T.; Sjöblom, Magnus; Hodge, David B.; Rova, Ulrika.

In: Bioresource Technology, Vol. 176, 01.01.2015, p. 71-79.

Research output: Contribution to journalArticle

Kudahettige-Nilsson, RL, Helmerius, J, Nilsson, RT, Sjöblom, M, Hodge, DB & Rova, U 2015, 'Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor' Bioresource Technology, vol. 176, pp. 71-79. DOI: 10.1016/j.biortech.2014.11.012
Kudahettige-Nilsson RL, Helmerius J, Nilsson RT, Sjöblom M, Hodge DB, Rova U. Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. Bioresource Technology. 2015 Jan 1;176:71-79. Available from, DOI: 10.1016/j.biortech.2014.11.012
Kudahettige-Nilsson, Rasika L. ; Helmerius, Jonas ; Nilsson, Robert T. ; Sjöblom, Magnus ; Hodge, David B. ; Rova, Ulrika. / Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. In: Bioresource Technology. 2015 ; Vol. 176. pp. 71-79
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