Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor

Rasika L. Kudahettige-Nilsson, Jonas Helmerius, Robert T. Nilsson, Magnus Sjöblom, David B. Hodge, Ulrika Rova

    Research output: Contribution to journalArticle

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    Abstract

    Acetone-butanol-ethanol (ABE) fermentation was studied using acid-hydrolyzed xylan recovered from hardwood Kraft black liquor by CO2 acidification as the only carbon source. Detoxification of hydrolyzate using activated carbon was conducted to evaluate the impact of inhibitor removal and fermentation. Xylose hydrolysis yields as high as 18.4% were demonstrated at the highest severity hydrolysis condition. Detoxification using active carbon was effective for removal of both phenolics (76-81%) and HMF (38-52%). Batch fermentation of the hydrolyzate and semi-defined P2 media resulted in a total solvent yield of 0.12-0.13g/g and 0.34g/g, corresponding to a butanol concentration of 1.8-2.1g/L and 7.3g/L respectively. This work is the first study of a process for the production of a biologically-derived biofuel from hemicelluloses solubilized during Kraft pulping and demonstrates the feasibility of utilizing xylan recovered directly from industrial Kraft pulping liquors as a feedstock for biological production of biofuels such as butanol.

    Original languageEnglish (US)
    Pages (from-to)71-79
    Number of pages9
    JournalBioresource Technology
    Volume176
    DOIs
    StatePublished - Jan 1 2015

    Profile

    Butenes
    Fermentation
    Clostridium acetobutylicum
    Betula
    Butanols
    Xylose
    Carbon
    fermentation
    Kraft process
    Detoxification
    Biofuels
    Hydrolysis
    Removal
    Xylans
    detoxification
    biofuel
    hydrolysis
    carbon
    Marsupialia
    Clostridium

    Keywords

    • ABE fermentation
    • Birch wood kraft black liquor
    • Detoxification
    • Fermentation inhibitors
    • Lignin precipitation

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal
    • Medicine(all)

    Cite this

    Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. / Kudahettige-Nilsson, Rasika L.; Helmerius, Jonas; Nilsson, Robert T.; Sjöblom, Magnus; Hodge, David B.; Rova, Ulrika.

    In: Bioresource Technology, Vol. 176, 01.01.2015, p. 71-79.

    Research output: Contribution to journalArticle

    Kudahettige-Nilsson RL, Helmerius J, Nilsson RT, Sjöblom M, Hodge DB, Rova U. Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. Bioresource Technology. 2015 Jan 1;176:71-79. Available from, DOI: 10.1016/j.biortech.2014.11.012

    Kudahettige-Nilsson, Rasika L.; Helmerius, Jonas; Nilsson, Robert T.; Sjöblom, Magnus; Hodge, David B.; Rova, Ulrika / Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor.

    In: Bioresource Technology, Vol. 176, 01.01.2015, p. 71-79.

    Research output: Contribution to journalArticle

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