Biomimetic interfaces for a multifunctional biosensor array microsystem

Brian Hassler, R. Mark Worden, Andrew Mason, Peter Kim, Neeraj Kohli, J. Gregory Zeikus, Maris Laivenieks, Robert Ofoli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

  • 10 Citations

Abstract

Bioelectronic interfaces that allow dehydrogenase enzymes to electrically communicate with electrodes have potential applications in the development of biosensors and biocatalytic reactors. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode, has been developed. Also described is a tri-functional linking molecule that binds the mediator and cofactor to the electrode in a unique spatial arrangement in which the dehydrogenase enzyme can bind to cofactor and multistep electron transfer between the electrode and enzyme is achieved. This approach provides greater flexibility in assembling complex bioelectronic interfaces than is possible with previously reported, linear linking molecules. A cysteine molecule was self-assembled on a gold electrode via a thiol bond. The electron mediator toluidine blue O (TBO) and the cofactor, β-nicotinamide adenine dinucleotide phosphate (NADP+) were chemically attached to cysteine via the formation of amide bonds. Cyclic voltammetry, was used to demonstrate the electrical activity, and enzymatic activity of the resulting bioelectronic interface.

LanguageEnglish (US)
Title of host publicationProceedings of IEEE Sensors
EditorsD. Rocha, P.M. Sarro, M.J. Vellekoop
Pages991-994
Number of pages4
Volume2
StatePublished - 2004
EventIEEE Sensors 2004 - Vienna, Austria
Duration: Oct 24 2004Oct 27 2004

Other

OtherIEEE Sensors 2004
CountryAustria
CityVienna
Period10/24/0410/27/04

Profile

Microsystems
Biomimetics
Biosensors
Electrodes
Enzymes
Molecules
Electrons
Amides
Cyclic voltammetry
Phosphates
Gold
Sensors

Keywords

  • Biomimetic interfaces
  • Biosensor array
  • Dehydrogenase
  • Electrochemical sensor
  • Lipid bilayer
  • Membrane protein

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Hassler, B., Worden, R. M., Mason, A., Kim, P., Kohli, N., Zeikus, J. G., ... Ofoli, R. (2004). Biomimetic interfaces for a multifunctional biosensor array microsystem. In D. Rocha, P. M. Sarro, & M. J. Vellekoop (Eds.), Proceedings of IEEE Sensors (Vol. 2, pp. 991-994). [T4L-C.4]

Biomimetic interfaces for a multifunctional biosensor array microsystem. / Hassler, Brian; Worden, R. Mark; Mason, Andrew; Kim, Peter; Kohli, Neeraj; Zeikus, J. Gregory; Laivenieks, Maris; Ofoli, Robert.

Proceedings of IEEE Sensors. ed. / D. Rocha; P.M. Sarro; M.J. Vellekoop. Vol. 2 2004. p. 991-994 T4L-C.4.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hassler, B, Worden, RM, Mason, A, Kim, P, Kohli, N, Zeikus, JG, Laivenieks, M & Ofoli, R 2004, Biomimetic interfaces for a multifunctional biosensor array microsystem. in D Rocha, PM Sarro & MJ Vellekoop (eds), Proceedings of IEEE Sensors. vol. 2, T4L-C.4, pp. 991-994, IEEE Sensors 2004, Vienna, Austria, 10/24/04.
Hassler B, Worden RM, Mason A, Kim P, Kohli N, Zeikus JG et al. Biomimetic interfaces for a multifunctional biosensor array microsystem. In Rocha D, Sarro PM, Vellekoop MJ, editors, Proceedings of IEEE Sensors. Vol. 2. 2004. p. 991-994. T4L-C.4.
Hassler, Brian ; Worden, R. Mark ; Mason, Andrew ; Kim, Peter ; Kohli, Neeraj ; Zeikus, J. Gregory ; Laivenieks, Maris ; Ofoli, Robert. / Biomimetic interfaces for a multifunctional biosensor array microsystem. Proceedings of IEEE Sensors. editor / D. Rocha ; P.M. Sarro ; M.J. Vellekoop. Vol. 2 2004. pp. 991-994
@inproceedings{c2ca0fc35b294c71bda57741c871060c,
title = "Biomimetic interfaces for a multifunctional biosensor array microsystem",
abstract = "Bioelectronic interfaces that allow dehydrogenase enzymes to electrically communicate with electrodes have potential applications in the development of biosensors and biocatalytic reactors. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode, has been developed. Also described is a tri-functional linking molecule that binds the mediator and cofactor to the electrode in a unique spatial arrangement in which the dehydrogenase enzyme can bind to cofactor and multistep electron transfer between the electrode and enzyme is achieved. This approach provides greater flexibility in assembling complex bioelectronic interfaces than is possible with previously reported, linear linking molecules. A cysteine molecule was self-assembled on a gold electrode via a thiol bond. The electron mediator toluidine blue O (TBO) and the cofactor, β-nicotinamide adenine dinucleotide phosphate (NADP+) were chemically attached to cysteine via the formation of amide bonds. Cyclic voltammetry, was used to demonstrate the electrical activity, and enzymatic activity of the resulting bioelectronic interface.",
keywords = "Biomimetic interfaces, Biosensor array, Dehydrogenase, Electrochemical sensor, Lipid bilayer, Membrane protein",
author = "Brian Hassler and Worden, {R. Mark} and Andrew Mason and Peter Kim and Neeraj Kohli and Zeikus, {J. Gregory} and Maris Laivenieks and Robert Ofoli",
year = "2004",
language = "English (US)",
volume = "2",
pages = "991--994",
editor = "D. Rocha and P.M. Sarro and M.J. Vellekoop",
booktitle = "Proceedings of IEEE Sensors",

}

TY - GEN

T1 - Biomimetic interfaces for a multifunctional biosensor array microsystem

AU - Hassler,Brian

AU - Worden,R. Mark

AU - Mason,Andrew

AU - Kim,Peter

AU - Kohli,Neeraj

AU - Zeikus,J. Gregory

AU - Laivenieks,Maris

AU - Ofoli,Robert

PY - 2004

Y1 - 2004

N2 - Bioelectronic interfaces that allow dehydrogenase enzymes to electrically communicate with electrodes have potential applications in the development of biosensors and biocatalytic reactors. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode, has been developed. Also described is a tri-functional linking molecule that binds the mediator and cofactor to the electrode in a unique spatial arrangement in which the dehydrogenase enzyme can bind to cofactor and multistep electron transfer between the electrode and enzyme is achieved. This approach provides greater flexibility in assembling complex bioelectronic interfaces than is possible with previously reported, linear linking molecules. A cysteine molecule was self-assembled on a gold electrode via a thiol bond. The electron mediator toluidine blue O (TBO) and the cofactor, β-nicotinamide adenine dinucleotide phosphate (NADP+) were chemically attached to cysteine via the formation of amide bonds. Cyclic voltammetry, was used to demonstrate the electrical activity, and enzymatic activity of the resulting bioelectronic interface.

AB - Bioelectronic interfaces that allow dehydrogenase enzymes to electrically communicate with electrodes have potential applications in the development of biosensors and biocatalytic reactors. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode, has been developed. Also described is a tri-functional linking molecule that binds the mediator and cofactor to the electrode in a unique spatial arrangement in which the dehydrogenase enzyme can bind to cofactor and multistep electron transfer between the electrode and enzyme is achieved. This approach provides greater flexibility in assembling complex bioelectronic interfaces than is possible with previously reported, linear linking molecules. A cysteine molecule was self-assembled on a gold electrode via a thiol bond. The electron mediator toluidine blue O (TBO) and the cofactor, β-nicotinamide adenine dinucleotide phosphate (NADP+) were chemically attached to cysteine via the formation of amide bonds. Cyclic voltammetry, was used to demonstrate the electrical activity, and enzymatic activity of the resulting bioelectronic interface.

KW - Biomimetic interfaces

KW - Biosensor array

KW - Dehydrogenase

KW - Electrochemical sensor

KW - Lipid bilayer

KW - Membrane protein

UR - http://www.scopus.com/inward/record.url?scp=27944456108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27944456108&partnerID=8YFLogxK

M3 - Conference contribution

VL - 2

SP - 991

EP - 994

BT - Proceedings of IEEE Sensors

ER -