Biomimetic interfaces for a multifunctional biosensor array microsystem

Brian Hassler, R. Mark Worden, Andrew Mason, Peter Kim, Neeraj Kohli, J. Gregory Zeikus, Maris Laivenieks, Robert Ofoli

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    • 10 Citations

    Abstract

    Bioelectronic interfaces that allow dehydrogenase enzymes to electrically communicate with electrodes have potential applications in the development of biosensors and biocatalytic reactors. A fully scalable, post-CMOS-compatible, three-electrode interface to biochemical sensors, consisting of Ti/Au working and auxiliary electrodes and a Ti/Au/Ag/AgCl reference electrode, has been developed. Also described is a tri-functional linking molecule that binds the mediator and cofactor to the electrode in a unique spatial arrangement in which the dehydrogenase enzyme can bind to cofactor and multistep electron transfer between the electrode and enzyme is achieved. This approach provides greater flexibility in assembling complex bioelectronic interfaces than is possible with previously reported, linear linking molecules. A cysteine molecule was self-assembled on a gold electrode via a thiol bond. The electron mediator toluidine blue O (TBO) and the cofactor, β-nicotinamide adenine dinucleotide phosphate (NADP+) were chemically attached to cysteine via the formation of amide bonds. Cyclic voltammetry, was used to demonstrate the electrical activity, and enzymatic activity of the resulting bioelectronic interface.

    Original languageEnglish (US)
    Title of host publicationProceedings of IEEE Sensors
    EditorsD. Rocha, P.M. Sarro, M.J. Vellekoop
    Pages991-994
    Number of pages4
    Volume2
    StatePublished - 2004
    EventIEEE Sensors 2004 - Vienna, Austria

    Other

    OtherIEEE Sensors 2004
    CountryAustria
    CityVienna
    Period10/24/0410/27/04

    Profile

    Electrodes
    Enzymes
    Molecules
    Biosensors
    Electrons
    Microsystems
    Biomimetics
    Amides
    Cyclic voltammetry
    Phosphates
    Gold
    Sensors

    Keywords

    • Biomimetic interfaces
    • Biosensor array
    • Dehydrogenase
    • Electrochemical sensor
    • Lipid bilayer
    • Membrane protein

    ASJC Scopus subject areas

    • Engineering (miscellaneous)
    • Electrical and Electronic Engineering

    Cite this

    Hassler, B., Worden, R. M., Mason, A., Kim, P., Kohli, N., Zeikus, J. G., ... Ofoli, R. (2004). Biomimetic interfaces for a multifunctional biosensor array microsystem. In D. Rocha, P. M. Sarro, & M. J. Vellekoop (Eds.), Proceedings of IEEE Sensors (Vol. 2, pp. 991-994). [T4L-C.4]

    Biomimetic interfaces for a multifunctional biosensor array microsystem. / Hassler, Brian; Worden, R. Mark; Mason, Andrew; Kim, Peter; Kohli, Neeraj; Zeikus, J. Gregory; Laivenieks, Maris; Ofoli, Robert.

    Proceedings of IEEE Sensors. ed. / D. Rocha; P.M. Sarro; M.J. Vellekoop. Vol. 2 2004. p. 991-994 T4L-C.4.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Hassler, B, Worden, RM, Mason, A, Kim, P, Kohli, N, Zeikus, JG, Laivenieks, M & Ofoli, R 2004, Biomimetic interfaces for a multifunctional biosensor array microsystem. in D Rocha, PM Sarro & MJ Vellekoop (eds), Proceedings of IEEE Sensors. vol. 2, T4L-C.4, pp. 991-994, IEEE Sensors 2004, Vienna, Austria, 24-27 October.
    Hassler B, Worden RM, Mason A, Kim P, Kohli N, Zeikus JG et al. Biomimetic interfaces for a multifunctional biosensor array microsystem. In Rocha D, Sarro PM, Vellekoop MJ, editors, Proceedings of IEEE Sensors. Vol. 2. 2004. p. 991-994. T4L-C.4.

    Hassler, Brian; Worden, R. Mark; Mason, Andrew; Kim, Peter; Kohli, Neeraj; Zeikus, J. Gregory; Laivenieks, Maris; Ofoli, Robert / Biomimetic interfaces for a multifunctional biosensor array microsystem.

    Proceedings of IEEE Sensors. ed. / D. Rocha; P.M. Sarro; M.J. Vellekoop. Vol. 2 2004. p. 991-994 T4L-C.4.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

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