Biomimetic interfaces for characterizing membrane proteins

Neeraj Kohli, Sachin S. Vaidya, Robert Y. Ofoli, Ilsoon Lee, Robert M. Worden, Devesh Srivastava, Rudy Richardson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cell membranes consisting predominantly of a bilayer lipid membrane (BLM) and membrane proteins efficiently perform many vital sensing, signaling, catalytic, and bioelectronic processes at the molecular scale. Although membrane proteins comprise 25-30% of the human genome and 30-50% of top drug targets, their analysis lags far behind that of soluble proteins due to difficulties in expressing, purifying, and reconstituting membrane proteins in an active conformation. Recently, Michigan State University (MSU) has developed special facilities and expertise to express recombinant membrane proteins and embed them into biomimetic interfaces in which their activities may be expressed. This paper will describe fabrication of functional and nanostructured biomimetic interfaces based on BLM and polyelectrolytes and application of the interfaces to study the medically relevant membrane proteins such as Neuropathy Target Esterase (NTE). These interfaces have outstanding potential for conducting fundamental studies of nanoscale biological processes and for developing new membrane-protein-based technologies relevant to pharmaceutical industries, including high-throughput drug screening systems and novel biosensors.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages5291
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Profile

Proteins
Membranes
Biomimetics
Lipid bilayers
Cell membranes
Polyelectrolytes
Biosensors
Drug products
Conformations
Screening
Genes
Throughput
Fabrication
Industry

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kohli, N., Vaidya, S. S., Ofoli, R. Y., Lee, I., Worden, R. M., Srivastava, D., & Richardson, R. (2005). Biomimetic interfaces for characterizing membrane proteins. In AIChE Annual Meeting, Conference Proceedings (pp. 5291)

Biomimetic interfaces for characterizing membrane proteins. / Kohli, Neeraj; Vaidya, Sachin S.; Ofoli, Robert Y.; Lee, Ilsoon; Worden, Robert M.; Srivastava, Devesh; Richardson, Rudy.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 5291.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kohli, N, Vaidya, SS, Ofoli, RY, Lee, I, Worden, RM, Srivastava, D & Richardson, R 2005, Biomimetic interfaces for characterizing membrane proteins. in AIChE Annual Meeting, Conference Proceedings. pp. 5291, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 30-4 November.
Kohli N, Vaidya SS, Ofoli RY, Lee I, Worden RM, Srivastava D et al. Biomimetic interfaces for characterizing membrane proteins. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 5291.

Kohli, Neeraj; Vaidya, Sachin S.; Ofoli, Robert Y.; Lee, Ilsoon; Worden, Robert M.; Srivastava, Devesh; Richardson, Rudy / Biomimetic interfaces for characterizing membrane proteins.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 5291.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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