Brachypodium as an experimental system for the study of stem parenchyma biology in grasses

Jacob Krüger Jensen, Curtis Gene Wilkerson

    Research output: Contribution to journalArticle

    Abstract

    Stem parenchyma is a major cell type that serves key metabolic functions for the plant especially in large grasses, such as sugarcane and sweet sorghum, where it serves to store sucrose or other products of photosynthesis. It is therefore desirable to understand the metabolism of this cell type as well as the mechanisms by which it provides its function for the rest of the plant. Ultimately, this information can be used to selectively manipulate this cell type in a controlled manner to achieve crop improvement. In this study, we show that Brachypodium distachyon is a useful model system for stem pith parenchyma biology. Brachypodium can be grown under condition where it resembles the growth patterns of important crops in that it produces large amounts of stem material with the lower leaves senescing and with significant stores of photosynthate located in the stem parenchyma cell types. We further characterize stem plastid morphology as a function of tissue types, as this organelle is central for a number of metabolic pathways, and quantify gene expression for the four main classes of starch biosynthetic genes. Notably, we find several of these genes differentially regulated between stem and leaf. These studies show, consistent with other grasses, that the stem functions as a specialized storage compartment in Brachypodium.

    Original languageEnglish (US)
    Article numbere0173095
    JournalPLoS ONE
    Volume12
    Issue number3
    DOIs
    StatePublished - Mar 1 2017

    Profile

    Brachypodium
    Poaceae
    stems
    Genes
    Cyclic AMP Receptor Protein
    Autistic Disorder
    Crops
    parenchyma
    cells
    grasses
    Saccharum
    Plastids
    Sorghum
    Photosynthesis
    Metabolic Networks and Pathways
    Organelles
    Starch
    Sucrose
    Stem Cells
    Gene Expression

    ASJC Scopus subject areas

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Brachypodium as an experimental system for the study of stem parenchyma biology in grasses. / Jensen, Jacob Krüger; Wilkerson, Curtis Gene.

    In: PLoS ONE, Vol. 12, No. 3, e0173095, 01.03.2017.

    Research output: Contribution to journalArticle

    Jensen, Jacob Krüger; Wilkerson, Curtis Gene / Brachypodium as an experimental system for the study of stem parenchyma biology in grasses.

    In: PLoS ONE, Vol. 12, No. 3, e0173095, 01.03.2017.

    Research output: Contribution to journalArticle

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