Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds

Daniel A. Lynam, Dena Shahriari, Kayla J. Wolf, Phillip A. Angart, Jacob Koffler, Mark H. Tuszynski, Christina Chan, Patrick Walton, Jeffrey Sakamoto

    Research output: Contribution to journalArticle

    • 3 Citations

    Abstract

    Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10 days in consistent dosages averaging 80.5 ± 12.5 (SD) ng/mL. Moreover, the BDNF released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages.

    Original languageEnglish (US)
    Pages (from-to)128-131
    Number of pages4
    JournalActa Biomaterialia
    Volume18
    DOIs
    StatePublished - May 1 2015

    Profile

    Brain-Derived Neurotrophic Factor
    Sepharose
    Brain
    Hydrogen
    Scaffolds
    Scaffolds (biology)
    Nerve Regeneration
    Hydrogel
    Central Nervous System
    Cell Proliferation
    In Vitro Techniques
    Cell proliferation
    Neurology
    Hydrogels
    Rats
    Assays
    Cells
    Staphylococcal Pneumonia
    Beryllium
    Castration

    Keywords

    • Agarose
    • BDNF
    • Controlled release
    • Hydrogel
    • Nerve

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Biotechnology
    • Biochemistry
    • Molecular Biology

    Cite this

    Lynam, D. A., Shahriari, D., Wolf, K. J., Angart, P. A., Koffler, J., Tuszynski, M. H., ... Sakamoto, J. (2015). Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. Acta Biomaterialia, 18, 128-131. DOI: 10.1016/j.actbio.2015.02.014

    Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. / Lynam, Daniel A.; Shahriari, Dena; Wolf, Kayla J.; Angart, Phillip A.; Koffler, Jacob; Tuszynski, Mark H.; Chan, Christina; Walton, Patrick; Sakamoto, Jeffrey.

    In: Acta Biomaterialia, Vol. 18, 01.05.2015, p. 128-131.

    Research output: Contribution to journalArticle

    Lynam, DA, Shahriari, D, Wolf, KJ, Angart, PA, Koffler, J, Tuszynski, MH, Chan, C, Walton, P & Sakamoto, J 2015, 'Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds' Acta Biomaterialia, vol 18, pp. 128-131. DOI: 10.1016/j.actbio.2015.02.014
    Lynam DA, Shahriari D, Wolf KJ, Angart PA, Koffler J, Tuszynski MH et al. Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. Acta Biomaterialia. 2015 May 1;18:128-131. Available from, DOI: 10.1016/j.actbio.2015.02.014

    Lynam, Daniel A.; Shahriari, Dena; Wolf, Kayla J.; Angart, Phillip A.; Koffler, Jacob; Tuszynski, Mark H.; Chan, Christina; Walton, Patrick; Sakamoto, Jeffrey / Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds.

    In: Acta Biomaterialia, Vol. 18, 01.05.2015, p. 128-131.

    Research output: Contribution to journalArticle

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    abstract = "Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10 days in consistent dosages averaging 80.5 ± 12.5 (SD) ng/mL. Moreover, the BDNF released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages.",
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    AU - Koffler,Jacob

    AU - Tuszynski,Mark H.

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