Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds

Daniel A. Lynam, Dena Shahriari, Kayla J. Wolf, Phillip A. Angart, Jacob Koffler, Mark H. Tuszynski, Christina Chan, Patrick Walton, Jeffrey Sakamoto

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10 days in consistent dosages averaging 80.5 ± 12.5 (SD) ng/mL. Moreover, the BDNF released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages.

LanguageEnglish (US)
Pages128-131
Number of pages4
JournalActa Biomaterialia
Volume18
DOIs
StatePublished - May 1 2015

Profile

Brain-Derived Neurotrophic Factor
Scaffolds
Sepharose
Brain
Hydrogen
Scaffolds (biology)
Nerve Regeneration
Hydrogel
Cell proliferation
Neurology
Hydrogels
Rats
Assays
Central Nervous System
Cells
Cell Proliferation
Nerve Growth Factors

Keywords

  • Agarose
  • BDNF
  • Controlled release
  • Hydrogel
  • Nerve

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Lynam, D. A., Shahriari, D., Wolf, K. J., Angart, P. A., Koffler, J., Tuszynski, M. H., ... Sakamoto, J. (2015). Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. Acta Biomaterialia, 18, 128-131. DOI: 10.1016/j.actbio.2015.02.014

Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. / Lynam, Daniel A.; Shahriari, Dena; Wolf, Kayla J.; Angart, Phillip A.; Koffler, Jacob; Tuszynski, Mark H.; Chan, Christina; Walton, Patrick; Sakamoto, Jeffrey.

In: Acta Biomaterialia, Vol. 18, 01.05.2015, p. 128-131.

Research output: Contribution to journalArticle

Lynam, DA, Shahriari, D, Wolf, KJ, Angart, PA, Koffler, J, Tuszynski, MH, Chan, C, Walton, P & Sakamoto, J 2015, 'Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds' Acta Biomaterialia, vol 18, pp. 128-131. DOI: 10.1016/j.actbio.2015.02.014
Lynam DA, Shahriari D, Wolf KJ, Angart PA, Koffler J, Tuszynski MH et al. Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. Acta Biomaterialia. 2015 May 1;18:128-131. Available from, DOI: 10.1016/j.actbio.2015.02.014
Lynam, Daniel A. ; Shahriari, Dena ; Wolf, Kayla J. ; Angart, Phillip A. ; Koffler, Jacob ; Tuszynski, Mark H. ; Chan, Christina ; Walton, Patrick ; Sakamoto, Jeffrey. / Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. In: Acta Biomaterialia. 2015 ; Vol. 18. pp. 128-131
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