Brain region-specificity of palmitic acid-induced abnormalities associated with Alzheimer's disease

Sachin Patil, Deebika Balu, Joseph Melrose, Christina Chan

    Research output: Contribution to journalArticle

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    Abstract

    Background. Alzheimer's disease (AD) is a progressive, neurodegenerative disease mostly affecting the basal forebrain, cortex and hippocampus whereas the cerebellum is relatively spared. The reason behind this region-specific brain damage in AD is not well understood. Here, we report our data suggesting "differential free fatty acid metabolism in the different brain areas" as a potentially important factor in causing the region-specific damage observed in AD brain. Findings. The astroglia from two different rat brain regions, cortex (region affected in AD) and cerebellum (unaffected region), were treated with 0.2 mM of palmitic acid. The conditioned media were then transferred to the cortical neurons to study the possible effects on the two main, AD-associated protein abnormalities, viz. BACE1 upregulation and hyperphosphorylation of tau. The conditioned media from palmitic-acid treated cortical astroglia, but not the cerebellar astroglia, significantly elevated levels of phosphorylated tau and BACE1 in cortical neurons as compared to controls (47 7% and 45 4%, respectively). Conclusion. The present data provide an experimental explanation for the region-specific damage observed in AD brain; higher fatty acid-metabolizing capacity of cortical astroglia as compared to cerebellar astroglia, may play a causal role in increasing vulnerability of cortex in AD, while sparing cerebellum.

    Original languageEnglish (US)
    Article number20
    JournalBMC Research Notes
    Volume1
    DOIs
    StatePublished - 2008

    Profile

    Palmitic Acid
    Alzheimer Disease
    Brain
    Supravalvular Aortic Stenosis
    Astrocytes
    Cerebellum
    Conditioned Culture Medium
    Neurons
    Nitrogenase
    Nonesterified Fatty Acids
    Neurodegenerative Diseases
    Hippocampus
    Up-Regulation
    Fatty Acids
    Proteins
    Basal Forebrain
    Staphylococcal Pneumonia
    Blood Flow Velocity
    Submandibular Gland
    Metabolism

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Brain region-specificity of palmitic acid-induced abnormalities associated with Alzheimer's disease. / Patil, Sachin; Balu, Deebika; Melrose, Joseph; Chan, Christina.

    In: BMC Research Notes, Vol. 1, 20, 2008.

    Research output: Contribution to journalArticle

    Patil, Sachin; Balu, Deebika; Melrose, Joseph; Chan, Christina / Brain region-specificity of palmitic acid-induced abnormalities associated with Alzheimer's disease.

    In: BMC Research Notes, Vol. 1, 20, 2008.

    Research output: Contribution to journalArticle

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    abstract = "Background. Alzheimer's disease (AD) is a progressive, neurodegenerative disease mostly affecting the basal forebrain, cortex and hippocampus whereas the cerebellum is relatively spared. The reason behind this region-specific brain damage in AD is not well understood. Here, we report our data suggesting {"}differential free fatty acid metabolism in the different brain areas{"} as a potentially important factor in causing the region-specific damage observed in AD brain. Findings. The astroglia from two different rat brain regions, cortex (region affected in AD) and cerebellum (unaffected region), were treated with 0.2 mM of palmitic acid. The conditioned media were then transferred to the cortical neurons to study the possible effects on the two main, AD-associated protein abnormalities, viz. BACE1 upregulation and hyperphosphorylation of tau. The conditioned media from palmitic-acid treated cortical astroglia, but not the cerebellar astroglia, significantly elevated levels of phosphorylated tau and BACE1 in cortical neurons as compared to controls (47 7% and 45 4%, respectively). Conclusion. The present data provide an experimental explanation for the region-specific damage observed in AD brain; higher fatty acid-metabolizing capacity of cortical astroglia as compared to cerebellar astroglia, may play a causal role in increasing vulnerability of cortex in AD, while sparing cerebellum.",
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