CAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells

Linxia Zhang, Linsey C. Seitz, Amy M. Abramczyk, Li Liu, Christina Chan

    Research output: Contribution to journalArticle

    • 23 Citations

    Abstract

    The intracellular second messenger cAMP is frequently used in induction media to induce mesenchymal stem cells (MSCs) into neural lineage cells. To date, an understanding of the role cAMP exerts on MSCs and whether cAMP can induce MSCs into functional neurons is still lacking. We found cAMP initiated neuron-like morphology changes early and neural differentiation much later. The early phase changes in morphology were due to cell shrinkage, which subsequently rendered some cells apoptotic. While the morphology changes occurred prior to the expression of neural markers, it is not required for neural marker expression and the two processes are differentially regulated downstream of cAMP-activated protein kinase A. cAMP enabled MSCs to gain neural marker expressions with neuronal function, such as, calcium rise in response to neuronal activators, dopamine, glutamate, and potassium chloride. However, only some of the cells induced by cAMP responded to the three neuronal activators and further lack the neuronal morphology, suggesting that although cAMP is able to direct MSCs towards neural differentiation, they do not achieve terminal differentiation.

    Original languageEnglish (US)
    Pages (from-to)863-876
    Number of pages14
    JournalCellular and Molecular Life Sciences
    Volume68
    Issue number5
    DOIs
    StatePublished - Mar 2011

    Profile

    Mesenchymal Stromal Cells
    Neurons
    Potassium Chloride
    Second Messenger Systems
    Cyclic AMP-Dependent Protein Kinases
    Glutamic Acid
    Dopamine
    Calcium

    Keywords

    • Apoptosis
    • cAMP
    • Morphology
    • Neural differentiation

    ASJC Scopus subject areas

    • Cell Biology
    • Molecular Biology
    • Molecular Medicine
    • Pharmacology
    • Cellular and Molecular Neuroscience

    Cite this

    CAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells. / Zhang, Linxia; Seitz, Linsey C.; Abramczyk, Amy M.; Liu, Li; Chan, Christina.

    In: Cellular and Molecular Life Sciences, Vol. 68, No. 5, 03.2011, p. 863-876.

    Research output: Contribution to journalArticle

    Zhang, Linxia; Seitz, Linsey C.; Abramczyk, Amy M.; Liu, Li; Chan, Christina / CAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.

    In: Cellular and Molecular Life Sciences, Vol. 68, No. 5, 03.2011, p. 863-876.

    Research output: Contribution to journalArticle

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