Cell adhesion on polyelectrolyte multilayer coated polydimethylsiloxane surfaces with varying topographies

Srivatsan Kidambi, Natasha Udpa, Stacey A. Schroeder, Robert Findlan, Ilsoon Lee, Christina Chan

    Research output: Contribution to journalArticle

    • 53 Citations

    Abstract

    This article demonstrates that the micro-topography of the surface with respect to the pattern size and pitch influences cell adhesion and proliferation. Extensive research has shown the dependence of cell proliferation on substrate chemistry, but the influence of substrate topography on cell attachment has only recently been appreciated. To evaluate the effect of substrate physical properties (i.e., periodic microstructures) on cell attachment and morphology, we compared the response of several cell types (fibroblasts, HeLa, and primary hepatocytes) cultured on various polydimethylsiloxane (PDMS) patterns. PDMS has been used as an artificial construct to mimic biological structures. Although PDMS is widely used in biomedical applications, membrane technology, and microlithography, it is difficult to maintain cells on PDMS for long periods, and the polymer has proved to be a relatively inefficient substrate for cell adhesion. To improve adhesion, we built polyelectrolyte multilayers (PEMs) on PDMS surfaces to increase surface wettability, thereby improving attachment and spreading of the cells. Micrographs demonstrate the cellular response to physical parameters, such as pattern size and pitch, and suggest that surface topography, in part, regulates cell adhesion and proliferation. Therefore, varying the surface topography may provide a method to influence cell attachment and proliferation for tissue-engineering applications.

    Original languageEnglish (US)
    Pages (from-to)2105-2117
    Number of pages13
    JournalTissue Engineering
    Volume13
    Issue number8
    DOIs
    StatePublished - Aug 2007

    Profile

    Polydimethylsiloxane
    Cell Adhesion
    Cell Proliferation
    Cell adhesion
    Substrates
    Cell proliferation
    Topography
    Surface topography
    Polyelectrolytes
    Multilayers
    Wettability
    Tissue Engineering
    Hepatocytes
    Polymers
    Fibroblasts
    Technology
    Membranes
    Membrane technology
    Tissue engineering
    Lithography

    ASJC Scopus subject areas

    • Biophysics
    • Cell Biology
    • Biotechnology

    Cite this

    Cell adhesion on polyelectrolyte multilayer coated polydimethylsiloxane surfaces with varying topographies. / Kidambi, Srivatsan; Udpa, Natasha; Schroeder, Stacey A.; Findlan, Robert; Lee, Ilsoon; Chan, Christina.

    In: Tissue Engineering, Vol. 13, No. 8, 08.2007, p. 2105-2117.

    Research output: Contribution to journalArticle

    Kidambi, Srivatsan; Udpa, Natasha; Schroeder, Stacey A.; Findlan, Robert; Lee, Ilsoon; Chan, Christina / Cell adhesion on polyelectrolyte multilayer coated polydimethylsiloxane surfaces with varying topographies.

    In: Tissue Engineering, Vol. 13, No. 8, 08.2007, p. 2105-2117.

    Research output: Contribution to journalArticle

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