Cell adhesive behavior on thin polyelectrolyte multilayers: Cells attempt to achieve homeostasis of its adhesion energy

Sumit Mehrotra, S. Christopher Hunley, Kendell M. Pawelec, Linxia Zhang, Ilsoon Lee, Seungik Baek, Christina Chan

Research output: Contribution to journalArticle

  • 39 Citations

Abstract

Linearly growing ultrathin polyelectrolyte multilayer (PEM) films of strong polyelectrolytes, poly(diallyldimethylammonium chloride) (PDAC), and sulfonated polystyrene, sodium salt (SPS) exhibit a gradual shift from cytophilic to cytophobic behavior, with increasing thickness for films of less than 100 nm. Previous explanations based on film hydration, swelling, and changes in the elastic modulus cannot account for the cytophobicity observed with these thin films as the number of bilayers increases. We implemented a finite element analysis to help elucidate the observed trends in cell spreading. The simulation results suggest that cells maintain a constant level of energy consumption (energy homeostasis) during active probing and thus respond to changes in the film stiffness as the film thickness increases by adjusting their morphology and the number of focal adhesions recruited and thereby their attachment to a substrate.

LanguageEnglish (US)
Pages12794-12802
Number of pages9
JournalLangmuir
Volume26
Issue number15
DOIs
StatePublished - Aug 3 2010

Profile

homeostasis
Polyelectrolytes
Adhesives
adhesives
Multilayers
adhesion
Homeostasis
Adhesion
Finite Element Analysis
Focal Adhesions
Elastic Modulus
Polystyrenes
cells
Salts
Sodium
Multilayer films
Hydration
Swelling
Film thickness
energy

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy
  • Medicine(all)

Cite this

Cell adhesive behavior on thin polyelectrolyte multilayers : Cells attempt to achieve homeostasis of its adhesion energy. / Mehrotra, Sumit; Hunley, S. Christopher; Pawelec, Kendell M.; Zhang, Linxia; Lee, Ilsoon; Baek, Seungik; Chan, Christina.

In: Langmuir, Vol. 26, No. 15, 03.08.2010, p. 12794-12802.

Research output: Contribution to journalArticle

Mehrotra S, Hunley SC, Pawelec KM, Zhang L, Lee I, Baek S et al. Cell adhesive behavior on thin polyelectrolyte multilayers: Cells attempt to achieve homeostasis of its adhesion energy. Langmuir. 2010 Aug 3;26(15):12794-12802. Available from, DOI: 10.1021/la101689z
Mehrotra, Sumit ; Hunley, S. Christopher ; Pawelec, Kendell M. ; Zhang, Linxia ; Lee, Ilsoon ; Baek, Seungik ; Chan, Christina. / Cell adhesive behavior on thin polyelectrolyte multilayers : Cells attempt to achieve homeostasis of its adhesion energy. In: Langmuir. 2010 ; Vol. 26, No. 15. pp. 12794-12802
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