Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods

Namita Bansal, Aditya Bhalla, Sivakumar Pattathil, Sara L. Adelman, Michael G. Hahn, David B. Hodge, Eric L. Hegg

Research output: Contribution to journalArticle

  • 5 Citations

Abstract

The responses of four diverse hardwoods (hybrid poplar, silver birch, hybrid aspen, and sugar maple) to alkaline hydrogen peroxide (AHP) pretreated at ambient temperature and pressure were analyzed to gain a deeper understanding of the cell wall properties that contribute to differences in enzymatic hydrolysis efficacy following alkaline-oxidative pretreatment. The enzymatic hydrolysis yields of these diverse hardwoods increased significantly with increasing the cell wall-associated, redox-active transition metal content. These increases in hydrolysis yields were directly correlated with improved delignification. Furthermore, we demonstrated that these improvements in hydrolysis yields could be achieved either through elevated levels of naturally-occurring metals, namely Cu, Fe, and Mn, or by the addition of a homogeneous transition metal catalyst (e.g. Cu 2,2′-bipyridine complexes) capable of penetrating into the cell wall matrix. Removal of naturally-occurring cell wall-associated transition metals by chelation resulted in substantial decreases in the hydrolysis yields following AHP pretreatment, while re-addition of metals in the form of Cu 2,2′-bipyridine complexes and to a limited extent Fe 2,2′-bipyridine complexes prior to pretreatment restored the improved hydrolysis yields. Glycome profiles showed improved extractability of xylan, xyloglucan, and pectin epitopes with increasing hydrolysis yields for the diverse hardwoods subjected to the alkaline-oxidative pretreatment, demonstrating that the strength of association between cell wall matrix polymers decreased as a consequence of improved delignification.

LanguageEnglish (US)
Pages1405-1415
Number of pages11
JournalGreen Chemistry
Volume18
Issue number5
DOIs
StatePublished - 2016

Profile

Hardwoods
transition element
Transition metals
hydrolysis
Hydrolysis
2,2'-Dipyridyl
Cells
Delignification
Enzymatic hydrolysis
Hydrogen peroxide
Hydrogen Peroxide
Metals
hydrogen peroxide
Epitopes
Xylans
Chelation
Polymer matrix
Silver
Sugars
chelation

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

Cite this

Bansal, N., Bhalla, A., Pattathil, S., Adelman, S. L., Hahn, M. G., Hodge, D. B., & Hegg, E. L. (2016). Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods. Green Chemistry, 18(5), 1405-1415. DOI: 10.1039/c5gc01748c

Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods. / Bansal, Namita; Bhalla, Aditya; Pattathil, Sivakumar; Adelman, Sara L.; Hahn, Michael G.; Hodge, David B.; Hegg, Eric L.

In: Green Chemistry, Vol. 18, No. 5, 2016, p. 1405-1415.

Research output: Contribution to journalArticle

Bansal, N, Bhalla, A, Pattathil, S, Adelman, SL, Hahn, MG, Hodge, DB & Hegg, EL 2016, 'Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods' Green Chemistry, vol. 18, no. 5, pp. 1405-1415. DOI: 10.1039/c5gc01748c
Bansal N, Bhalla A, Pattathil S, Adelman SL, Hahn MG, Hodge DB et al. Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods. Green Chemistry. 2016;18(5):1405-1415. Available from, DOI: 10.1039/c5gc01748c
Bansal, Namita ; Bhalla, Aditya ; Pattathil, Sivakumar ; Adelman, Sara L. ; Hahn, Michael G. ; Hodge, David B. ; Hegg, Eric L./ Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods. In: Green Chemistry. 2016 ; Vol. 18, No. 5. pp. 1405-1415
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