Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces

Ying Liu, Serban F. Peteu, R. Mark Worden

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Peroxynitrite (ONOO- or PON for short) and its associated radical products are clinically important reactive nitrogen oxide species that play key roles in several devastating diseases. Some PON-induced disease processes involve lipid oxidation or nitration. Peroxidation of phospholipids can compromise a biomembrane's integrity and alter its fluidity and electrochemical resistance. The inherent complexity of intact biomembranes makes investigation of PON-induced damage challenging. The use of biomimetic interfaces consisting of synthetic bilayer lipid membranes (BLM) that have a known composition enables controlled studies to be performed and hypotheses to be tested. A variety of characterization tools are available to study the effect of PON on such biomimetic interfaces. Traditional electrophysiology approaches can be used to study transient pore formation in unsupported, planar BLM. Application of electrochemical impedance spectroscopy to BLM that are tethered to electrodes can be used to measure biomembrane capacitance and ion permeability. Optical methods based on hydrophobic fluorescent probes that partition into biomembranes can provide parallel measurements. Simultaneous use of multiple characterization tools could provide real-time, label-free measurements of PON concentration and its effect on biomembrane properties. This chapter summarizes mechanisms by which PON damages membrane lipids, describes common biomimetic interface platforms and electrochemical and optical techniques used to characterize them, and demonstrates a novel application of a biomimetic interface to measure the effect of PON exposure on a biomembrane's electrochemical resistance.

LanguageEnglish (US)
Title of host publicationPeroxynitrite Detection in Biological Media: Challenges and Advances
PublisherRoyal Society of Chemistry
Pages151-165
Number of pages15
Volume2016-January
Edition7
DOIs
StatePublished - 2016

Publication series

NameRSC Detection Science
No.7
Volume2016-January
ISSN (Print)20523068
ISSN (Electronic)20523076

Profile

Biomimetics
Peroxynitrous Acid
Membrane Lipids
Lipid bilayers
Lipid Bilayers
lipid
membrane
Electrochemical Techniques
Electrophysiology
Dielectric Spectroscopy
Nitration
Reactive Nitrogen Species
Fluidity
Phospholipids
Nitrogen oxides
optical method
Electrochemical impedance spectroscopy
Fluorescent Dyes
damage
Lipids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Bioengineering
  • Chemistry(all)
  • Environmental Chemistry
  • Pollution
  • Biochemistry, medical
  • Pathology and Forensic Medicine

Cite this

Liu, Y., Peteu, S. F., & Worden, R. M. (2016). Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. In Peroxynitrite Detection in Biological Media: Challenges and Advances (7 ed., Vol. 2016-January, pp. 151-165). (RSC Detection Science; Vol. 2016-January, No. 7). Royal Society of Chemistry. DOI: 10.1039/9781782622352-00151

Chapter 8 : Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. / Liu, Ying; Peteu, Serban F.; Worden, R. Mark.

Peroxynitrite Detection in Biological Media: Challenges and Advances. Vol. 2016-January 7. ed. Royal Society of Chemistry, 2016. p. 151-165 (RSC Detection Science; Vol. 2016-January, No. 7).

Research output: Chapter in Book/Report/Conference proceedingChapter

Liu, Y, Peteu, SF & Worden, RM 2016, Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. in Peroxynitrite Detection in Biological Media: Challenges and Advances. 7 edn, vol. 2016-January, RSC Detection Science, no. 7, vol. 2016-January, Royal Society of Chemistry, pp. 151-165. DOI: 10.1039/9781782622352-00151
Liu Y, Peteu SF, Worden RM. Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. In Peroxynitrite Detection in Biological Media: Challenges and Advances. 7 ed. Vol. 2016-January. Royal Society of Chemistry. 2016. p. 151-165. (RSC Detection Science; 7). Available from, DOI: 10.1039/9781782622352-00151
Liu, Ying ; Peteu, Serban F. ; Worden, R. Mark. / Chapter 8 : Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. Peroxynitrite Detection in Biological Media: Challenges and Advances. Vol. 2016-January 7. ed. Royal Society of Chemistry, 2016. pp. 151-165 (RSC Detection Science; 7).
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