Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces

Ying Liu, Serban F. Peteu, R. Mark Worden

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Peroxynitrite (ONOO- or PON for short) and its associated radical products are clinically important reactive nitrogen oxide species that play key roles in several devastating diseases. Some PON-induced disease processes involve lipid oxidation or nitration. Peroxidation of phospholipids can compromise a biomembrane's integrity and alter its fluidity and electrochemical resistance. The inherent complexity of intact biomembranes makes investigation of PON-induced damage challenging. The use of biomimetic interfaces consisting of synthetic bilayer lipid membranes (BLM) that have a known composition enables controlled studies to be performed and hypotheses to be tested. A variety of characterization tools are available to study the effect of PON on such biomimetic interfaces. Traditional electrophysiology approaches can be used to study transient pore formation in unsupported, planar BLM. Application of electrochemical impedance spectroscopy to BLM that are tethered to electrodes can be used to measure biomembrane capacitance and ion permeability. Optical methods based on hydrophobic fluorescent probes that partition into biomembranes can provide parallel measurements. Simultaneous use of multiple characterization tools could provide real-time, label-free measurements of PON concentration and its effect on biomembrane properties. This chapter summarizes mechanisms by which PON damages membrane lipids, describes common biomimetic interface platforms and electrochemical and optical techniques used to characterize them, and demonstrates a novel application of a biomimetic interface to measure the effect of PON exposure on a biomembrane's electrochemical resistance.

    Original languageEnglish (US)
    Title of host publicationPeroxynitrite Detection in Biological Media: Challenges and Advances
    PublisherRoyal Society of Chemistry
    Pages151-165
    Number of pages15
    Volume2016-January
    Edition7
    DOIs
    StatePublished - 2016

    Publication series

    NameRSC Detection Science
    Number7
    Volume2016-January
    ISSN (Print)20523068
    ISSN (Electronic)20523076

    Profile

    Biomimetics
    Peroxynitrous Acid
    Membrane Lipids
    lipid
    Autoantibodies
    membrane
    Lipid Bilayers
    Lipid bilayers
    damage
    Buccal Administration
    Supravalvular Aortic Stenosis
    Electrochemical Techniques
    Dielectric Spectroscopy
    Reactive Nitrogen Species
    Electrophysiology
    Fluorescent Dyes
    Permeability
    Phospholipids
    Nitric Oxide
    Electrodes

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Bioengineering
    • Chemistry(all)
    • Environmental Chemistry
    • Pollution
    • Biochemistry, medical
    • Pathology and Forensic Medicine

    Cite this

    Liu, Y., Peteu, S. F., & Worden, R. M. (2016). Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. In Peroxynitrite Detection in Biological Media: Challenges and Advances (7 ed., Vol. 2016-January, pp. 151-165). (RSC Detection Science; Vol. 2016-January, No. 7). Royal Society of Chemistry. DOI: 10.1039/9781782622352-00151

    Chapter 8 : Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. / Liu, Ying; Peteu, Serban F.; Worden, R. Mark.

    Peroxynitrite Detection in Biological Media: Challenges and Advances. Vol. 2016-January 7. ed. Royal Society of Chemistry, 2016. p. 151-165 (RSC Detection Science; Vol. 2016-January, No. 7).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Liu, Y, Peteu, SF & Worden, RM 2016, Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. in Peroxynitrite Detection in Biological Media: Challenges and Advances. 7 edn, vol. 2016-January, RSC Detection Science, no. 7, vol. 2016-January, Royal Society of Chemistry, pp. 151-165. DOI: 10.1039/9781782622352-00151
    Liu Y, Peteu SF, Worden RM. Chapter 8: Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces. In Peroxynitrite Detection in Biological Media: Challenges and Advances. 7 ed. Vol. 2016-January. Royal Society of Chemistry. 2016. p. 151-165. (RSC Detection Science; 7). Available from, DOI: 10.1039/9781782622352-00151

    Liu, Ying; Peteu, Serban F.; Worden, R. Mark / Chapter 8 : Investigation of peroxynitrite- biomembrane interactions using biomimetic interfaces.

    Peroxynitrite Detection in Biological Media: Challenges and Advances. Vol. 2016-January 7. ed. Royal Society of Chemistry, 2016. p. 151-165 (RSC Detection Science; Vol. 2016-January, No. 7).

    Research output: Chapter in Book/Report/Conference proceedingChapter

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