Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization

Deboleena Chakraborty, Erik McClellan, Robert Hasselbeck, Scott Calabrese Barton

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

Homogeneous, sub-micron thick bioactive films containing redox hydrogels and the enzyme laccase were prepared on gold-coated glass slides via convective self-assembly. Sub-micron film thickness (∼10-50 nm) allowed higher mediator and enzyme utilization by lowering transport limitations. This study demonstrates control of redox hydrogel film morphology by manipulating hydrophilicity, spreading and wetting properties of the hydrogel/electrode interface. Use of a non-ionic surfactant (Triton X-100) enabled spreading of enzyme containing precursor solutions with no negative impact on enzyme activity. Ellipsometry was used to measure films-welling properties of these systems, square wave voltammetry was employed to estimate the amount of electroactive redox polymer, and cyclic voltammetry enabled estimation of apparent electron diffusivity. This study impacts the design of thin, catalytic films for bioelectronic applications.

LanguageEnglish (US)
PagesH3076-H3082
JournalJournal of the Electrochemical Society
Volume161
Issue number13
DOIs
StatePublished - 2014

Profile

Hydrogel
Hydrogels
enzymes
Enzymes
Thin films
Electrodes
electrodes
thin films
Laccase
Nonionic surfactants
Ellipsometry
Octoxynol
Hydrophilicity
Enzyme activity
Voltammetry
Gold
Self assembly
enzyme activity
Cyclic voltammetry
Film thickness

ASJC Scopus subject areas

  • Electrochemistry
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Renewable Energy, Sustainability and the Environment
  • Condensed Matter Physics

Cite this

Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization. / Chakraborty, Deboleena; McClellan, Erik; Hasselbeck, Robert; Barton, Scott Calabrese.

In: Journal of the Electrochemical Society, Vol. 161, No. 13, 2014, p. H3076-H3082.

Research output: Contribution to journalArticle

Chakraborty, Deboleena ; McClellan, Erik ; Hasselbeck, Robert ; Barton, Scott Calabrese. / Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization. In: Journal of the Electrochemical Society. 2014 ; Vol. 161, No. 13. pp. H3076-H3082
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