Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization

Deboleena Chakraborty, Erik McClellan, Robert Hasselbeck, Scott Calabrese Barton

    Research output: Research - peer-reviewArticle

    • 5 Citations

    Abstract

    Homogeneous, sub-micron thick bioactive films containing redox hydrogels and the enzyme laccase were prepared on gold-coated glass slides via convective self-assembly. Sub-micron film thickness (∼10-50 nm) allowed higher mediator and enzyme utilization by lowering transport limitations. This study demonstrates control of redox hydrogel film morphology by manipulating hydrophilicity, spreading and wetting properties of the hydrogel/electrode interface. Use of a non-ionic surfactant (Triton X-100) enabled spreading of enzyme containing precursor solutions with no negative impact on enzyme activity. Ellipsometry was used to measure films-welling properties of these systems, square wave voltammetry was employed to estimate the amount of electroactive redox polymer, and cyclic voltammetry enabled estimation of apparent electron diffusivity. This study impacts the design of thin, catalytic films for bioelectronic applications.

    LanguageEnglish (US)
    PagesH3076-H3082
    JournalJournal of the Electrochemical Society
    Volume161
    Issue number13
    DOIs
    StatePublished - 2014

    Profile

    Hydrogel
    Thin films
    Electrodes
    Enzymes
    Oxidation-Reduction
    Hydrogels
    enzymes
    electrodes
    thin films
    Laccase
    Nonionic surfactants
    Ellipsometry
    Octoxynol
    Hydrophilicity
    Enzyme activity
    Voltammetry
    Gold
    Self assembly
    Cyclic voltammetry
    Film thickness

    ASJC Scopus subject areas

    • Electrochemistry
    • Electronic, Optical and Magnetic Materials
    • Materials Chemistry
    • Surfaces, Coatings and Films
    • Renewable Energy, Sustainability and the Environment
    • Condensed Matter Physics

    Cite this

    Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization. / Chakraborty, Deboleena; McClellan, Erik; Hasselbeck, Robert; Barton, Scott Calabrese.

    In: Journal of the Electrochemical Society, Vol. 161, No. 13, 2014, p. H3076-H3082.

    Research output: Research - peer-reviewArticle

    Chakraborty, Deboleena ; McClellan, Erik ; Hasselbeck, Robert ; Barton, Scott Calabrese. / Characterization of enzyme-redox hydrogel thin-film electrodes for improved utilization. In: Journal of the Electrochemical Society. 2014 ; Vol. 161, No. 13. pp. H3076-H3082
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