Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking

Dorit Granot, Michael K. Nkansah, Margaret F. Bennewitz, Kevin S. Tang, Eleni A. Markakis, Erik M. Shapiro

Research output: Research - peer-reviewArticle

  • 10 Citations

Abstract

Purpose To design, fabricate, characterize, and in vivo assay clinically viable magnetic particles for MRI-based cell tracking. Methods Poly(lactide-co-glycolide) (PLGA) encapsulated magnetic nano and microparticles were fabricated. Multiple biologically relevant experiments were performed to assess cell viability, cellular performance, and stem cell differentiation. In vivo MRI experiments were performed to separately test cell transplantation and cell migration paradigms, as well as in vivo biodegradation. Results Highly magnetic nano (∼100 nm) and microparticles (∼1-2 μm) were fabricated. Magnetic cell labeling in culture occurred rapidly achieving 3-50 pg Fe/cell at 3 h for different particles types, and >100 pg Fe/cell after 10 h, without the requirement of a transfection agent, and with no effect on cell viability. The capability of magnetically labeled mesenchymal or neural stem cells to differentiate down multiple lineages, or for magnetically labeled immune cells to release cytokines following stimulation, was uncompromised. An in vivo biodegradation study revealed that NPs degraded ∼80% over the course of 12 weeks. MRI detected as few as 10 magnetically labeled cells, transplanted into the brains of rats. Also, these particles enabled the in vivo monitoring of endogenous neural progenitor cell migration in rat brains over 2 weeks. Conclusion The robust MRI properties and benign safety profile of these particles make them promising candidates for clinical translation for MRI-based cell tracking.

LanguageEnglish (US)
Pages1238-1250
Number of pages13
JournalMagnetic Resonance in Medicine
Volume71
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

Profile

Cell Tracking
Polyglactin 910
Cell Movement
Cell Survival
Stem Cells
Brain
Neural Stem Cells
Cell Transplantation
Mesenchymal Stromal Cells
Transfection
Cell Differentiation
Cytokines
Safety
polylactic acid-polyglycolic acid copolymer

Keywords

  • cellular MRI
  • iron oxide
  • microparticle
  • nanoparticle
  • stem cells

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Granot, D., Nkansah, M. K., Bennewitz, M. F., Tang, K. S., Markakis, E. A., & Shapiro, E. M. (2014). Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking. Magnetic Resonance in Medicine, 71(3), 1238-1250. DOI: 10.1002/mrm.24741

Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking. / Granot, Dorit; Nkansah, Michael K.; Bennewitz, Margaret F.; Tang, Kevin S.; Markakis, Eleni A.; Shapiro, Erik M.

In: Magnetic Resonance in Medicine, Vol. 71, No. 3, 03.2014, p. 1238-1250.

Research output: Research - peer-reviewArticle

Granot, D, Nkansah, MK, Bennewitz, MF, Tang, KS, Markakis, EA & Shapiro, EM 2014, 'Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking' Magnetic Resonance in Medicine, vol 71, no. 3, pp. 1238-1250. DOI: 10.1002/mrm.24741
Granot D, Nkansah MK, Bennewitz MF, Tang KS, Markakis EA, Shapiro EM. Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking. Magnetic Resonance in Medicine. 2014 Mar;71(3):1238-1250. Available from, DOI: 10.1002/mrm.24741
Granot, Dorit ; Nkansah, Michael K. ; Bennewitz, Margaret F. ; Tang, Kevin S. ; Markakis, Eleni A. ; Shapiro, Erik M./ Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking. In: Magnetic Resonance in Medicine. 2014 ; Vol. 71, No. 3. pp. 1238-1250
@article{41822d863b6f4fc0b8f7357b5f605976,
title = "Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking",
abstract = "Purpose To design, fabricate, characterize, and in vivo assay clinically viable magnetic particles for MRI-based cell tracking. Methods Poly(lactide-co-glycolide) (PLGA) encapsulated magnetic nano and microparticles were fabricated. Multiple biologically relevant experiments were performed to assess cell viability, cellular performance, and stem cell differentiation. In vivo MRI experiments were performed to separately test cell transplantation and cell migration paradigms, as well as in vivo biodegradation. Results Highly magnetic nano (∼100 nm) and microparticles (∼1-2 μm) were fabricated. Magnetic cell labeling in culture occurred rapidly achieving 3-50 pg Fe/cell at 3 h for different particles types, and >100 pg Fe/cell after 10 h, without the requirement of a transfection agent, and with no effect on cell viability. The capability of magnetically labeled mesenchymal or neural stem cells to differentiate down multiple lineages, or for magnetically labeled immune cells to release cytokines following stimulation, was uncompromised. An in vivo biodegradation study revealed that NPs degraded ∼80% over the course of 12 weeks. MRI detected as few as 10 magnetically labeled cells, transplanted into the brains of rats. Also, these particles enabled the in vivo monitoring of endogenous neural progenitor cell migration in rat brains over 2 weeks. Conclusion The robust MRI properties and benign safety profile of these particles make them promising candidates for clinical translation for MRI-based cell tracking.",
keywords = "cellular MRI, iron oxide, microparticle, nanoparticle, stem cells",
author = "Dorit Granot and Nkansah, {Michael K.} and Bennewitz, {Margaret F.} and Tang, {Kevin S.} and Markakis, {Eleni A.} and Shapiro, {Erik M.}",
year = "2014",
month = "3",
doi = "10.1002/mrm.24741",
volume = "71",
pages = "1238--1250",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Clinically viable magnetic poly(lactide-co-glycolide) particles for MRI-based cell tracking

AU - Granot,Dorit

AU - Nkansah,Michael K.

AU - Bennewitz,Margaret F.

AU - Tang,Kevin S.

AU - Markakis,Eleni A.

AU - Shapiro,Erik M.

PY - 2014/3

Y1 - 2014/3

N2 - Purpose To design, fabricate, characterize, and in vivo assay clinically viable magnetic particles for MRI-based cell tracking. Methods Poly(lactide-co-glycolide) (PLGA) encapsulated magnetic nano and microparticles were fabricated. Multiple biologically relevant experiments were performed to assess cell viability, cellular performance, and stem cell differentiation. In vivo MRI experiments were performed to separately test cell transplantation and cell migration paradigms, as well as in vivo biodegradation. Results Highly magnetic nano (∼100 nm) and microparticles (∼1-2 μm) were fabricated. Magnetic cell labeling in culture occurred rapidly achieving 3-50 pg Fe/cell at 3 h for different particles types, and >100 pg Fe/cell after 10 h, without the requirement of a transfection agent, and with no effect on cell viability. The capability of magnetically labeled mesenchymal or neural stem cells to differentiate down multiple lineages, or for magnetically labeled immune cells to release cytokines following stimulation, was uncompromised. An in vivo biodegradation study revealed that NPs degraded ∼80% over the course of 12 weeks. MRI detected as few as 10 magnetically labeled cells, transplanted into the brains of rats. Also, these particles enabled the in vivo monitoring of endogenous neural progenitor cell migration in rat brains over 2 weeks. Conclusion The robust MRI properties and benign safety profile of these particles make them promising candidates for clinical translation for MRI-based cell tracking.

AB - Purpose To design, fabricate, characterize, and in vivo assay clinically viable magnetic particles for MRI-based cell tracking. Methods Poly(lactide-co-glycolide) (PLGA) encapsulated magnetic nano and microparticles were fabricated. Multiple biologically relevant experiments were performed to assess cell viability, cellular performance, and stem cell differentiation. In vivo MRI experiments were performed to separately test cell transplantation and cell migration paradigms, as well as in vivo biodegradation. Results Highly magnetic nano (∼100 nm) and microparticles (∼1-2 μm) were fabricated. Magnetic cell labeling in culture occurred rapidly achieving 3-50 pg Fe/cell at 3 h for different particles types, and >100 pg Fe/cell after 10 h, without the requirement of a transfection agent, and with no effect on cell viability. The capability of magnetically labeled mesenchymal or neural stem cells to differentiate down multiple lineages, or for magnetically labeled immune cells to release cytokines following stimulation, was uncompromised. An in vivo biodegradation study revealed that NPs degraded ∼80% over the course of 12 weeks. MRI detected as few as 10 magnetically labeled cells, transplanted into the brains of rats. Also, these particles enabled the in vivo monitoring of endogenous neural progenitor cell migration in rat brains over 2 weeks. Conclusion The robust MRI properties and benign safety profile of these particles make them promising candidates for clinical translation for MRI-based cell tracking.

KW - cellular MRI

KW - iron oxide

KW - microparticle

KW - nanoparticle

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=84894484566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894484566&partnerID=8YFLogxK

U2 - 10.1002/mrm.24741

DO - 10.1002/mrm.24741

M3 - Article

VL - 71

SP - 1238

EP - 1250

JO - Magnetic Resonance in Medicine

T2 - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 3

ER -