Comparison of liposomes formed by sonication and extrusion: Rotational and translational diffusion of an embedded chromophore

Monique M. Lapinski, Angelines Castro-Forero, Aaron J. Greiner, Robert Y. Ofoli, Gary J. Blanchard

Research output: Contribution to journalArticle

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Abstract

We report on the physical and optical characterization of liposomes formed by extrusion and sonication, two widely used methods for vesicle preparation. We also address the issue of whether the properties of bilayers formed from liposomes prepared by the two techniques differ at the molecular and mesoscopic levels. We used the phospholipid 1,2-dioleoyl-sn;-glycero-3-phosphocholine (DOPC), with and without cholesterol, to form liposomes, incorporating 1-oleoy1-2-[ 12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-in-glycero- 3-phosphocholine (18:1-12:0 NBDPC) as an optical probe of dynamics. We measured the physical morphology of liposomes by transmission electron microscopy (TEM) and dynamic light scattering (DLS), and the rotational and translational diffusion of 18:1-12:0 NBD-PC by time correlated single photon counting (TCSPC) and fluorescence recovery after pattern photobleaching (FRAPP), respectively. We find that, despite apparent differences in average size and size distribution, both methods of preparation produced liposomes that exhibit the same molecular scale environment. The translational diffusion behavior of the tethered chromophore in planar bilayer lipid membranes formed from the two types of liposomes also yielded similar results.

LanguageEnglish (US)
Pages11677-11683
Number of pages7
JournalLangmuir
Volume23
Issue number23
DOIs
StatePublished - Nov 6 2007

Profile

Sonication
Liposomes
Chromophores
chromophores
Extrusion
preparation
cholesterol
lipids
counting
light scattering
Phosphorylcholine
recovery
membranes
fluorescence
transmission electron microscopy
probes
photons
Photobleaching
Lipid bilayers
Cholesterol

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Comparison of liposomes formed by sonication and extrusion : Rotational and translational diffusion of an embedded chromophore. / Lapinski, Monique M.; Castro-Forero, Angelines; Greiner, Aaron J.; Ofoli, Robert Y.; Blanchard, Gary J.

In: Langmuir, Vol. 23, No. 23, 06.11.2007, p. 11677-11683.

Research output: Contribution to journalArticle

Lapinski, Monique M. ; Castro-Forero, Angelines ; Greiner, Aaron J. ; Ofoli, Robert Y. ; Blanchard, Gary J./ Comparison of liposomes formed by sonication and extrusion : Rotational and translational diffusion of an embedded chromophore. In: Langmuir. 2007 ; Vol. 23, No. 23. pp. 11677-11683
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