Comparison of liposomes formed by sonication and extrusion: Rotational and translational diffusion of an embedded chromophore

Monique M. Lapinski, Angelines Castro-Forero, Aaron J. Greiner, Robert Y. Ofoli, Gary J. Blanchard

    Research output: Contribution to journalArticle

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    Abstract

    We report on the physical and optical characterization of liposomes formed by extrusion and sonication, two widely used methods for vesicle preparation. We also address the issue of whether the properties of bilayers formed from liposomes prepared by the two techniques differ at the molecular and mesoscopic levels. We used the phospholipid 1,2-dioleoyl-sn;-glycero-3-phosphocholine (DOPC), with and without cholesterol, to form liposomes, incorporating 1-oleoy1-2-[ 12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-in-glycero- 3-phosphocholine (18:1-12:0 NBDPC) as an optical probe of dynamics. We measured the physical morphology of liposomes by transmission electron microscopy (TEM) and dynamic light scattering (DLS), and the rotational and translational diffusion of 18:1-12:0 NBD-PC by time correlated single photon counting (TCSPC) and fluorescence recovery after pattern photobleaching (FRAPP), respectively. We find that, despite apparent differences in average size and size distribution, both methods of preparation produced liposomes that exhibit the same molecular scale environment. The translational diffusion behavior of the tethered chromophore in planar bilayer lipid membranes formed from the two types of liposomes also yielded similar results.

    Original languageEnglish (US)
    Pages (from-to)11677-11683
    Number of pages7
    JournalLangmuir
    Volume23
    Issue number23
    DOIs
    StatePublished - Nov 6 2007

    Profile

    Liposomes
    chromophores
    preparation
    Feline Sarcoma Viruses
    cholesterol
    lipids
    counting
    light scattering
    recovery
    membranes
    fluorescence
    transmission electron microscopy
    probes
    photons
    Sonication
    Chromophores
    Extrusion
    Dextrothyroxine
    Biological Adaptation
    Photobleaching

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

    Cite this

    Comparison of liposomes formed by sonication and extrusion : Rotational and translational diffusion of an embedded chromophore. / Lapinski, Monique M.; Castro-Forero, Angelines; Greiner, Aaron J.; Ofoli, Robert Y.; Blanchard, Gary J.

    In: Langmuir, Vol. 23, No. 23, 06.11.2007, p. 11677-11683.

    Research output: Contribution to journalArticle

    Lapinski, Monique M.; Castro-Forero, Angelines; Greiner, Aaron J.; Ofoli, Robert Y.; Blanchard, Gary J. / Comparison of liposomes formed by sonication and extrusion : Rotational and translational diffusion of an embedded chromophore.

    In: Langmuir, Vol. 23, No. 23, 06.11.2007, p. 11677-11683.

    Research output: Contribution to journalArticle

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