Computational design of novel protein binders and experimental affinity maturation

Timothy A. Whitehead, David Baker, Sarel J. Fleishman

    Research output: Chapter in Book/Report/Conference proceedingChapter

    • 16 Citations

    Abstract

    Computational design of novel protein binders has recently emerged as a useful technique to study biomolecular recognition and generate molecules for use in biotechnology, research, and biomedicine. Current limitations in computational design methodology have led to the adoption of high-throughput screening and affinity maturation techniques to diagnose modeling inaccuracies and generate high activity binders. Here, we scrutinize this combination of computational and experimental aspects and propose areas for future methodological improvements.

    Original languageEnglish (US)
    Title of host publicationMethods in Enzymology
    Pages1-19
    Number of pages19
    Volume523
    DOIs
    StatePublished - 2013

    Publication series

    NameMethods in Enzymology
    Volume523
    ISSN (Print)00766879
    ISSN (Electronic)15577988

    Profile

    Alginates
    Biotechnology
    Proteins
    Caprylates
    Viverridae
    Dental Materials
    Throughput
    Molecules

    Keywords

    • Affinity
    • Affinity maturation
    • Computational design
    • De novo design
    • Deep sequencing
    • Molecular recognition
    • Protein-protein interactions
    • Rosetta
    • Specificity
    • Yeast display

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    Whitehead, T. A., Baker, D., & Fleishman, S. J. (2013). Computational design of novel protein binders and experimental affinity maturation. In Methods in Enzymology (Vol. 523, pp. 1-19). (Methods in Enzymology; Vol. 523). DOI: 10.1016/B978-0-12-394292-0.00001-1

    Computational design of novel protein binders and experimental affinity maturation. / Whitehead, Timothy A.; Baker, David; Fleishman, Sarel J.

    Methods in Enzymology. Vol. 523 2013. p. 1-19 (Methods in Enzymology; Vol. 523).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Whitehead, TA, Baker, D & Fleishman, SJ 2013, Computational design of novel protein binders and experimental affinity maturation. in Methods in Enzymology. vol. 523, Methods in Enzymology, vol. 523, pp. 1-19. DOI: 10.1016/B978-0-12-394292-0.00001-1
    Whitehead TA, Baker D, Fleishman SJ. Computational design of novel protein binders and experimental affinity maturation. In Methods in Enzymology. Vol. 523. 2013. p. 1-19. (Methods in Enzymology). Available from, DOI: 10.1016/B978-0-12-394292-0.00001-1

    Whitehead, Timothy A.; Baker, David; Fleishman, Sarel J. / Computational design of novel protein binders and experimental affinity maturation.

    Methods in Enzymology. Vol. 523 2013. p. 1-19 (Methods in Enzymology; Vol. 523).

    Research output: Chapter in Book/Report/Conference proceedingChapter

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