Computational design of novel protein binders and experimental affinity maturation

Timothy A. Whitehead, David Baker, Sarel J. Fleishman

Research output: Chapter in Book/Report/Conference proceedingChapter

  • 17 Citations

Abstract

Computational design of novel protein binders has recently emerged as a useful technique to study biomolecular recognition and generate molecules for use in biotechnology, research, and biomedicine. Current limitations in computational design methodology have led to the adoption of high-throughput screening and affinity maturation techniques to diagnose modeling inaccuracies and generate high activity binders. Here, we scrutinize this combination of computational and experimental aspects and propose areas for future methodological improvements.

LanguageEnglish (US)
Title of host publicationMethods in Enzymology
Pages1-19
Number of pages19
Volume523
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume523
ISSN (Print)00766879
ISSN (Electronic)15577988

Profile

Biotechnology
Binders
Research
Screening
Proteins
Throughput
Molecules

Keywords

  • Affinity
  • Affinity maturation
  • Computational design
  • De novo design
  • Deep sequencing
  • Molecular recognition
  • Protein-protein interactions
  • Rosetta
  • Specificity
  • Yeast display

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Whitehead, T. A., Baker, D., & Fleishman, S. J. (2013). Computational design of novel protein binders and experimental affinity maturation. In Methods in Enzymology (Vol. 523, pp. 1-19). (Methods in Enzymology; Vol. 523). DOI: 10.1016/B978-0-12-394292-0.00001-1

Computational design of novel protein binders and experimental affinity maturation. / Whitehead, Timothy A.; Baker, David; Fleishman, Sarel J.

Methods in Enzymology. Vol. 523 2013. p. 1-19 (Methods in Enzymology; Vol. 523).

Research output: Chapter in Book/Report/Conference proceedingChapter

Whitehead, TA, Baker, D & Fleishman, SJ 2013, Computational design of novel protein binders and experimental affinity maturation. in Methods in Enzymology. vol. 523, Methods in Enzymology, vol. 523, pp. 1-19. DOI: 10.1016/B978-0-12-394292-0.00001-1
Whitehead TA, Baker D, Fleishman SJ. Computational design of novel protein binders and experimental affinity maturation. In Methods in Enzymology. Vol. 523. 2013. p. 1-19. (Methods in Enzymology). Available from, DOI: 10.1016/B978-0-12-394292-0.00001-1
Whitehead, Timothy A. ; Baker, David ; Fleishman, Sarel J./ Computational design of novel protein binders and experimental affinity maturation. Methods in Enzymology. Vol. 523 2013. pp. 1-19 (Methods in Enzymology).
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