Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Rosemary K. Le, Tyrone Wells, Parthapratim Das, Xianzhi Meng, Ryan J. Stoklosa, Aditya Bhalla, David B. Hodge, Joshua S. Yuan, Arthur J. Ragauskas

    Research output: Research - peer-reviewArticle

    • 5 Citations

    Abstract

    The bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719T. Here we show using 31P-NMR, HPAEC-PAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (∼70-300 g mol-1) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L-1 and accumulated 42.1% in oils based on cell dry weight after 48 h.

    LanguageEnglish (US)
    Pages4108-4115
    Number of pages8
    JournalRSC Advances
    Volume7
    Issue number7
    DOIs
    StatePublished - 2017

    Profile

    Waste treatment
    Biofuels
    Alkalies
    Biodiesel
    Cellulosic ethanol
    Bioconversion
    Lignin
    Peroxides
    Batch reactors
    Fermentation
    Feedstocks
    Effluents
    Bacteria
    Oils
    Molecular weight
    Nuclear magnetic resonance
    Lipids
    Glucose
    Degradation

    ASJC Scopus subject areas

    • Chemistry(all)
    • Chemical Engineering(all)

    Cite this

    Le, R. K., Wells, T., Das, P., Meng, X., Stoklosa, R. J., Bhalla, A., ... Ragauskas, A. J. (2017). Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. RSC Advances, 7(7), 4108-4115. DOI: 10.1039/C6RA28033A

    Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. / Le, Rosemary K.; Wells, Tyrone; Das, Parthapratim; Meng, Xianzhi; Stoklosa, Ryan J.; Bhalla, Aditya; Hodge, David B.; Yuan, Joshua S.; Ragauskas, Arthur J.

    In: RSC Advances, Vol. 7, No. 7, 2017, p. 4108-4115.

    Research output: Research - peer-reviewArticle

    Le, RK, Wells, T, Das, P, Meng, X, Stoklosa, RJ, Bhalla, A, Hodge, DB, Yuan, JS & Ragauskas, AJ 2017, 'Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci' RSC Advances, vol 7, no. 7, pp. 4108-4115. DOI: 10.1039/C6RA28033A
    Le RK, Wells T, Das P, Meng X, Stoklosa RJ, Bhalla A et al. Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. RSC Advances. 2017;7(7):4108-4115. Available from, DOI: 10.1039/C6RA28033A
    Le, Rosemary K. ; Wells, Tyrone ; Das, Parthapratim ; Meng, Xianzhi ; Stoklosa, Ryan J. ; Bhalla, Aditya ; Hodge, David B. ; Yuan, Joshua S. ; Ragauskas, Arthur J./ Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. In: RSC Advances. 2017 ; Vol. 7, No. 7. pp. 4108-4115
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