Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

Rosemary K. Le, Tyrone Wells, Parthapratim Das, Xianzhi Meng, Ryan J. Stoklosa, Aditya Bhalla, David B. Hodge, Joshua S. Yuan, Arthur J. Ragauskas

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

The bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719T. Here we show using 31P-NMR, HPAEC-PAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (∼70-300 g mol-1) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L-1 and accumulated 42.1% in oils based on cell dry weight after 48 h.

LanguageEnglish (US)
Pages4108-4115
Number of pages8
JournalRSC Advances
Volume7
Issue number7
DOIs
StatePublished - 2017

Profile

Waste treatment
Biofuels
Alkalies
Biodiesel
Cellulosic ethanol
Bioconversion
Lignin
Peroxides
Batch reactors
Fermentation
Feedstocks
Lipids
Glucose
Effluents
Bacteria
Oils
Molecular weight
Nuclear magnetic resonance
Degradation

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Le, R. K., Wells, T., Das, P., Meng, X., Stoklosa, R. J., Bhalla, A., ... Ragauskas, A. J. (2017). Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. RSC Advances, 7(7), 4108-4115. DOI: 10.1039/C6RA28033A

Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. / Le, Rosemary K.; Wells, Tyrone; Das, Parthapratim; Meng, Xianzhi; Stoklosa, Ryan J.; Bhalla, Aditya; Hodge, David B.; Yuan, Joshua S.; Ragauskas, Arthur J.

In: RSC Advances, Vol. 7, No. 7, 2017, p. 4108-4115.

Research output: Contribution to journalArticle

Le, RK, Wells, T, Das, P, Meng, X, Stoklosa, RJ, Bhalla, A, Hodge, DB, Yuan, JS & Ragauskas, AJ 2017, 'Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci' RSC Advances, vol 7, no. 7, pp. 4108-4115. DOI: 10.1039/C6RA28033A
Le RK, Wells T, Das P, Meng X, Stoklosa RJ, Bhalla A et al. Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. RSC Advances. 2017;7(7):4108-4115. Available from, DOI: 10.1039/C6RA28033A
Le, Rosemary K. ; Wells, Tyrone ; Das, Parthapratim ; Meng, Xianzhi ; Stoklosa, Ryan J. ; Bhalla, Aditya ; Hodge, David B. ; Yuan, Joshua S. ; Ragauskas, Arthur J./ Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci. In: RSC Advances. 2017 ; Vol. 7, No. 7. pp. 4108-4115
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