CREB modulates calcium signaling in cAMP-induced bone marrow stromal cells (BMSCs)

Linxia Zhang, Li Liu, Ryan Thompson, Christina Chan

    Research output: Contribution to journalArticle

    • 2 Citations

    Abstract

    Calcium signaling has a versatile role in many important cellular functions. Despite its importance, regulation of calcium signaling in bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) has not been explored extensively. Our previous study revealed that cyclic adenosine monophosphate (cAMP) enabled BMSCs to generate calcium signal upon stimulation by dopamine, KCl and glutamate. Concurrently, cAMP transiently activated the transcription factor cAMP response element binding protein (CREB) in BMSCs. Activity of CREB can be modulated by the calcium/calmodulin-dependent kinase signaling pathway, however, whether the calcium signaling observed in cAMP-induced BMSCs requires CREB has not been investigated. In an effort to uncover the role of CREB in the generation of calcium signaling in response to modulators such as dopamine and KCl, we knocked down CREB activity in BMSCs. Our study indicated that BMSCs, but not its close relative fibroblasts, are responsive to dopamine and KCl after cAMP treatment. Calcium signal elicited by dopamine depends, in part, on calcium influx whereas that elicited by KCl depends completely on calcium influx. Knock-down of CREB activity significantly reduced or abolished the cAMP-induced calcium response, and reintroducing a constitutively active CREB partially restored the calcium response.

    Original languageEnglish (US)
    Pages (from-to)257-268
    Number of pages12
    JournalCell Calcium
    Volume56
    Issue number4
    DOIs
    StatePublished - Oct 1 2014

    Profile

    Cyclic AMP Response Element-Binding Protein
    Calcium Signaling
    Mesenchymal Stromal Cells
    Cyclic AMP
    Calcium
    Dopamine
    Calcium-Calmodulin-Dependent Protein Kinases
    Response Elements
    Glutamic Acid
    Carrier Proteins
    Transcription Factors
    Fibroblasts
    Bone Marrow

    Keywords

    • Bone marrow stromal cells
    • Calcium signal
    • CREB
    • Morphology

    ASJC Scopus subject areas

    • Cell Biology
    • Molecular Biology
    • Physiology
    • Medicine(all)

    Cite this

    CREB modulates calcium signaling in cAMP-induced bone marrow stromal cells (BMSCs). / Zhang, Linxia; Liu, Li; Thompson, Ryan; Chan, Christina.

    In: Cell Calcium, Vol. 56, No. 4, 01.10.2014, p. 257-268.

    Research output: Contribution to journalArticle

    Zhang, Linxia; Liu, Li; Thompson, Ryan; Chan, Christina / CREB modulates calcium signaling in cAMP-induced bone marrow stromal cells (BMSCs).

    In: Cell Calcium, Vol. 56, No. 4, 01.10.2014, p. 257-268.

    Research output: Contribution to journalArticle

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