Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging

James P. Sumner, Richard Conroy, Erik M. Shapiro, John Moreland, Alan P. Koretsky

Research output: Contribution to journalArticle

  • 15 Citations

Abstract

Iron oxide particles are becoming an important contrast agent for magnetic resonance imaging (MRI) cell tracking studies. Simultaneous delivery of fluorescence indicators with the particles to individual cells offers the possibility of correlating optical images and MRI. In this work, it is demonstrated that micron-sized iron oxide particles (MPIOs) can be used as a carrier to deliver fluorescent probes to cells in culture as well as to migrating neural progenitors in vivo. Migrating progenitors were tracked with MRI and easily identified by histology because of the fluorescent probe. These data suggest that using MPIOs to deliver fluorescent probes should make it possible to combine MRI and optical imaging for in vivo cell tracking.

Original languageEnglish (US)
Article number051504
JournalJournal of Biomedical Optics
Volume12
Issue number5
DOIs
StatePublished - 2007
Externally publishedYes

Profile

Optical Imaging
Fluorescent Dyes
Oxides
Iron
Magnetic Resonance Imaging
magnetic resonance
Imaging techniques
Adenofibroma
probes
cells
Magnetic resonance
Guanine Nucleotides
iron oxides
Cell Tracking
Iron oxides
delivery
Contrast Media
Histology
Cell Culture Techniques
Fluorescence

Keywords

  • Fluorescent probes
  • Iron oxide particles
  • Magnetic resonance imaging
  • Optical imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials

Cite this

Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging. / Sumner, James P.; Conroy, Richard; Shapiro, Erik M.; Moreland, John; Koretsky, Alan P.

In: Journal of Biomedical Optics, Vol. 12, No. 5, 051504, 2007.

Research output: Contribution to journalArticle

Sumner, James P.; Conroy, Richard; Shapiro, Erik M.; Moreland, John; Koretsky, Alan P. / Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging.

In: Journal of Biomedical Optics, Vol. 12, No. 5, 051504, 2007.

Research output: Contribution to journalArticle

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