Designer synthetic media for studying microbialcatalyzed biofuel production

Xiaoyu Tang, Leonardo Da Costa Sousa, Mingjie Jin, Shishir P S Chundawat, Charles Kevin Chambliss, Ming W. Lau, Zeyi Xiao, Bruce E. Dale, Venkatesh Balan

    Research output: Research - peer-reviewArticle

    • 8 Citations

    Abstract

    Background: The fermentation inhibition of yeast or bacteria by lignocellulose-derived degradation products, during hexose/pentose co-fermentation, is a major bottleneck for cost-effective lignocellulosic biorefineries. To engineer microbial strains for improved performance, it is critical to understand the mechanisms of inhibition that affect fermentative organisms in the presence of major components of a lignocellulosic hydrolysate. The development of a synthetic lignocellulosic hydrolysate (SH) media with a composition similar to the actual biomass hydrolysate will be an important advancement to facilitate these studies. In this work, we characterized the nutrients and plant-derived decomposition products present in AFEX™ pretreated corn stover hydrolysate (ACH). The SH was formulated based on the ACH composition and was further used to evaluate the inhibitory effects of various families of decomposition products during Saccharomyces cerevisiae 424A (LNH-ST) fermentation. Results: The ACH contained high levels of nitrogenous compounds, notably amides, pyrazines, and imidazoles. In contrast, a relatively low content of furans and aromatic and aliphatic acids were found in the ACH. Though most of the families of decomposition products were inhibitory to xylose fermentation, due to their abundance, the nitrogenous compounds showed the most inhibition. From these compounds, amides (products of the ammonolysis reaction) contributed the most to the reduction of the fermentation performance. However, this result is associated to a concentration effect, as the corresponding carboxylic acids (products of hydrolysis) promoted greater inhibition when present at the same molar concentration as the amides. Due to its complexity, the formulated SH did not perfectly match the fermentation profile of the actual hydrolysate, especially the growth curve. However, the SH formulation was effective for studying the inhibitory effect of various compounds on yeast fermentation. Conclusions: The formulation of SHs is an important advancement for future multi-omics studies and for better understanding the mechanisms of fermentation inhibition in lignocellulosic hydrolysates. The SH formulated in this work was instrumental for defining the most important inhibitors in the ACH. Major AFEX decomposition products are less inhibitory to yeast fermentation than the products of dilute acid or steam explosion pretreatments; thus, ACH is readily fermentable by yeast without any detoxification.

    LanguageEnglish (US)
    Article number0179
    JournalBiotechnology for Biofuels
    Volume8
    Issue number1
    DOIs
    StatePublished - Jan 22 2015

    Profile

    biofuel
    fermentation
    Biofuels
    Fermentation
    product
    Yeast
    yeast
    decomposition
    Decomposition
    Yeasts
    effect
    Amides
    acid
    family
    Enzyme inhibition
    Acids
    Chemical analysis
    furan
    growth curve
    carboxylic acid

    Keywords

    • Afex
    • Amides inhibition
    • Carboxylic acids inhibition
    • Hydrolysate composition
    • Lignocellulose
    • Pretreatment decomposition products
    • Synthetic hydrolysate
    • Yeast fermentation inhibition

    ASJC Scopus subject areas

    • Energy(all)
    • Management, Monitoring, Policy and Law
    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Renewable Energy, Sustainability and the Environment

    Cite this

    Tang, X., Sousa, L. D. C., Jin, M., Chundawat, S. P. S., Chambliss, C. K., Lau, M. W., ... Balan, V. (2015). Designer synthetic media for studying microbialcatalyzed biofuel production. Biotechnology for Biofuels, 8(1), [0179]. DOI: 10.1186/s13068-014-0179-6

    Designer synthetic media for studying microbialcatalyzed biofuel production. / Tang, Xiaoyu; Sousa, Leonardo Da Costa; Jin, Mingjie; Chundawat, Shishir P S; Chambliss, Charles Kevin; Lau, Ming W.; Xiao, Zeyi; Dale, Bruce E.; Balan, Venkatesh.

    In: Biotechnology for Biofuels, Vol. 8, No. 1, 0179, 22.01.2015.

    Research output: Research - peer-reviewArticle

    Tang, X, Sousa, LDC, Jin, M, Chundawat, SPS, Chambliss, CK, Lau, MW, Xiao, Z, Dale, BE & Balan, V 2015, 'Designer synthetic media for studying microbialcatalyzed biofuel production' Biotechnology for Biofuels, vol 8, no. 1, 0179. DOI: 10.1186/s13068-014-0179-6
    Tang X, Sousa LDC, Jin M, Chundawat SPS, Chambliss CK, Lau MW et al. Designer synthetic media for studying microbialcatalyzed biofuel production. Biotechnology for Biofuels. 2015 Jan 22;8(1). 0179. Available from, DOI: 10.1186/s13068-014-0179-6
    Tang, Xiaoyu ; Sousa, Leonardo Da Costa ; Jin, Mingjie ; Chundawat, Shishir P S ; Chambliss, Charles Kevin ; Lau, Ming W. ; Xiao, Zeyi ; Dale, Bruce E. ; Balan, Venkatesh. / Designer synthetic media for studying microbialcatalyzed biofuel production. In: Biotechnology for Biofuels. 2015 ; Vol. 8, No. 1.
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