Development of a dual-aptamer-based multiplex protein biosensor

Shengnan Xie, S. Patrick Walton

Research output: Contribution to journalArticle

  • 21 Citations

Abstract

Parallel biosensors for proteins are becoming more essential for the thorough and systematic investigation of complex biological processes. These tools also enable improved clinical diagnoses relative to single-protein analyses due to their greater information content. If implemented correctly, affinity-based techniques can provide unique advantages in terms of sensitivity and flexibility. Aptamers are increasingly being used as the affinity reagents of choice for protein biosensing applications. Here, we describe the development and characterization of an aptamer-based method for parallel protein analyses that relies on recognition of the target protein by two unique aptamers targeting different epitopes on the protein. Our results show that the technique achieved simultaneous and quantitative detection of thrombin and platelet-derived growth factor-BB (PDGF-BB) with high specificity both in buffered solutions and in serum samples.

LanguageEnglish (US)
Pages2663-2668
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number12
DOIs
StatePublished - Aug 2010

Profile

Biosensing Techniques
Biosensors
Proteins
Epitopes
Biological Phenomena
Platelets
Thrombin
Serum

Keywords

  • Aptamer
  • Biosensor
  • Multiplex
  • PDGF-BB
  • Quantitative PCR
  • Thrombin

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Development of a dual-aptamer-based multiplex protein biosensor. / Xie, Shengnan; Walton, S. Patrick.

In: Biosensors and Bioelectronics, Vol. 25, No. 12, 08.2010, p. 2663-2668.

Research output: Contribution to journalArticle

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