Development of a dual-aptamer-based multiplex protein biosensor

Shengnan Xie, S. Patrick Walton

    Research output: Research - peer-reviewArticle

    • 17 Citations

    Abstract

    Parallel biosensors for proteins are becoming more essential for the thorough and systematic investigation of complex biological processes. These tools also enable improved clinical diagnoses relative to single-protein analyses due to their greater information content. If implemented correctly, affinity-based techniques can provide unique advantages in terms of sensitivity and flexibility. Aptamers are increasingly being used as the affinity reagents of choice for protein biosensing applications. Here, we describe the development and characterization of an aptamer-based method for parallel protein analyses that relies on recognition of the target protein by two unique aptamers targeting different epitopes on the protein. Our results show that the technique achieved simultaneous and quantitative detection of thrombin and platelet-derived growth factor-BB (PDGF-BB) with high specificity both in buffered solutions and in serum samples.

    LanguageEnglish (US)
    Pages2663-2668
    Number of pages6
    JournalBiosensors and Bioelectronics
    Volume25
    Issue number12
    DOIs
    StatePublished - Aug 2010

    Profile

    Biosensing Techniques
    Proteins
    Biosensors
    Biological Phenomena
    Thrombin
    Epitopes
    Serum
    platelet-derived growth factor BB
    Platelets
    Intercellular Signaling Peptides and Proteins

    Keywords

    • Aptamer
    • Biosensor
    • Multiplex
    • PDGF-BB
    • Quantitative PCR
    • Thrombin

    ASJC Scopus subject areas

    • Biophysics
    • Biomedical Engineering
    • Biotechnology
    • Electrochemistry

    Cite this

    Development of a dual-aptamer-based multiplex protein biosensor. / Xie, Shengnan; Walton, S. Patrick.

    In: Biosensors and Bioelectronics, Vol. 25, No. 12, 08.2010, p. 2663-2668.

    Research output: Research - peer-reviewArticle

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