Development of precipitant agents for precipitation of proteins based on hydrophobic interaction

E. A. Miranda, K. A. Berglund

    Research output: Contribution to journalArticle

    • 3 Citations

    Abstract

    Precipitation agents were developed to perform protein separation by precipitation based on hydrophobic interaction. They consisted of ligands, saturated linear chains of fatty acids, attached by esterification to a carrier molecule, methylcellulose. Precipitation of bovine serum albumin was achieved at 50 percent saturation of ammonium sulfate. The butyric acid derivative showed a higher efficiency in precipitating this protein than other derivatives tested. There is evidence that the interaction between the protein and the derivatives is hydrophobic.

    Original languageEnglish (US)
    Pages (from-to)1-12
    Number of pages12
    JournalBrazilian Journal of Chemical Engineering
    Volume12
    Issue number1
    StatePublished - 1995

    Profile

    Proteins
    Derivatives
    Dicarboxylic Amino Acids
    Cross Circulation
    Inborn Errors Amino Acid Metabolism
    Butyric acid
    Esterification
    Fatty acids
    Ligands
    Molecules
    Conjunctival Diseases
    Fusobacterium
    Chronic Hepatitis

    ASJC Scopus subject areas

    • Chemical Engineering(all)

    Cite this

    Development of precipitant agents for precipitation of proteins based on hydrophobic interaction. / Miranda, E. A.; Berglund, K. A.

    In: Brazilian Journal of Chemical Engineering, Vol. 12, No. 1, 1995, p. 1-12.

    Research output: Contribution to journalArticle

    Miranda, E. A.; Berglund, K. A. / Development of precipitant agents for precipitation of proteins based on hydrophobic interaction.

    In: Brazilian Journal of Chemical Engineering, Vol. 12, No. 1, 1995, p. 1-12.

    Research output: Contribution to journalArticle

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