Development of precipitant agents for precipitation of proteins based on hydrophobic interaction

E. A. Miranda, K. A. Berglund

Research output: Contribution to journalArticle

  • 3 Citations

Abstract

Precipitation agents were developed to perform protein separation by precipitation based on hydrophobic interaction. They consisted of ligands, saturated linear chains of fatty acids, attached by esterification to a carrier molecule, methylcellulose. Precipitation of bovine serum albumin was achieved at 50 percent saturation of ammonium sulfate. The butyric acid derivative showed a higher efficiency in precipitating this protein than other derivatives tested. There is evidence that the interaction between the protein and the derivatives is hydrophobic.

LanguageEnglish (US)
Pages1-12
Number of pages12
JournalBrazilian Journal of Chemical Engineering
Volume12
Issue number1
StatePublished - 1995

Profile

Derivatives
Proteins
Methylcellulose
Butyric acid
Butyric Acid
Esterification
Ammonium Sulfate
Bovine Serum Albumin
Fatty acids
Fatty Acids
Ligands
Molecules
Sulfates

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Development of precipitant agents for precipitation of proteins based on hydrophobic interaction. / Miranda, E. A.; Berglund, K. A.

In: Brazilian Journal of Chemical Engineering, Vol. 12, No. 1, 1995, p. 1-12.

Research output: Contribution to journalArticle

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