Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover

Mingjie Jin, Yanping Liu, Leonardo da Costa Sousa, Bruce E. Dale, Venkatesh Balan

Research output: Contribution to journalArticle

  • 1 Citations

Abstract

High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied rapid bioconversion with integrated recycle technology (RaBIT) and extractive ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24 h. Ethanol productivity was enhanced by two times and enzyme loading was reduced by 30%. Glucan and xylan conversions reached as high as 98% with an enzyme loading of as low as 8.4 mg protein per g glucan. The overall ethanol yield was 227 g ethanol/kg EA-CS (191 g ethanol/kg untreated CS). Biotechnol. Bioeng. 2017;114: 1713–1720.

LanguageEnglish (US)
Pages1713-1720
Number of pages8
JournalBiotechnology and Bioengineering
Volume114
Issue number8
DOIs
StatePublished - Aug 1 2017

Profile

Bioconversion
Ammonia
Zea mays
Ethanol
Technology
Enzymes
Enzymatic hydrolysis
Glucans
Fermentation
Hydrolysis
Productivity
Xylans
Recycling
Yeast
Biomass
Yeasts
Cells
Proteins
Costs and Cost Analysis
Costs

Keywords

  • EA pretreatment
  • enzymatic hydrolysis
  • enzyme recycling
  • ethanol fermentation
  • lignocellulosic ethanol
  • RaBIT process

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover. / Jin, Mingjie; Liu, Yanping; da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh.

In: Biotechnology and Bioengineering, Vol. 114, No. 8, 01.08.2017, p. 1713-1720.

Research output: Contribution to journalArticle

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