Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover

Mingjie Jin, Yanping Liu, Leonardo da Costa Sousa, Bruce E. Dale, Venkatesh Balan

    Research output: Contribution to journalArticle

    Abstract

    High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied rapid bioconversion with integrated recycle technology (RaBIT) and extractive ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24 h. Ethanol productivity was enhanced by two times and enzyme loading was reduced by 30%. Glucan and xylan conversions reached as high as 98% with an enzyme loading of as low as 8.4 mg protein per g glucan. The overall ethanol yield was 227 g ethanol/kg EA-CS (191 g ethanol/kg untreated CS). Biotechnol. Bioeng. 2017;114: 1713–1720.

    Original languageEnglish (US)
    Pages (from-to)1713-1720
    Number of pages8
    JournalBiotechnology and Bioengineering
    Volume114
    Issue number8
    DOIs
    StatePublished - Aug 1 2017

    Profile

    Ammonia
    Zea mays
    Ethanol
    Technology
    Enzymes
    Glucans
    Fermentation
    Hydrolysis
    Bioconversion
    Enzymatic hydrolysis
    Productivity
    Acyclic Acids
    Gravity Suits
    Butylene Glycols
    Xylans
    Recycling
    Biomass
    Yeasts
    Costs and Cost Analysis
    Proteins

    Keywords

    • EA pretreatment
    • enzymatic hydrolysis
    • enzyme recycling
    • ethanol fermentation
    • lignocellulosic ethanol
    • RaBIT process

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover. / Jin, Mingjie; Liu, Yanping; da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh.

    In: Biotechnology and Bioengineering, Vol. 114, No. 8, 01.08.2017, p. 1713-1720.

    Research output: Contribution to journalArticle

    Jin, Mingjie; Liu, Yanping; da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh / Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover.

    In: Biotechnology and Bioengineering, Vol. 114, No. 8, 01.08.2017, p. 1713-1720.

    Research output: Contribution to journalArticle

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