Differential iridoid production as revealed by a diversity panel of 84 cultivated and wild blueberry species

Courtney P. Leisner, Mohamed O. Kamileen, Megan E. Conway, Sarah E. O'Connor, C. Robin Buell

Research output: Contribution to journalArticle

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Abstract

Cultivated blueberry (Vaccinium corymbosum, Vaccinium angustifolium, Vaccinium darrowii, and Vaccinium virgatum) is an economically important fruit crop native to North America and a member of the Ericaceae family. Several species in the Ericaceae family including cranberry, lignonberry, bilberry, and neotropical blueberry species have been shown to produce iridoids, a class of pharmacologically important compounds present in over 15 plant families demonstrated to have a wide range of biological activities in humans including anticancer, anti-bacterial, and anti-inflammatory. While the antioxidant capacity of cultivated blueberry has been well studied, surveys of iridoid production in blueberry have been restricted to fruit of a very limited number of accessions of V. corymbosum, V. angustifolium and V. virgatum; none of these analyses have detected iridoids. To provide a broader survey of iridoid biosynthesis in cultivated blueberry, we constructed a panel of 84 accessions representing a wide range of cultivated market classes, as well as wild blueberry species, and surveyed these for the presence of iridoids. We identified the iridoid glycoside monotropein in fruits and leaves of all 13 wild Vaccinium species, yet only five of the 71 cultivars. Monotropein positive cultivars all had recent introgressions from wild species, suggesting that iridoid production can be targeted through breeding efforts that incorporate wild germplasm. A series of diverse developmental tissues was also surveyed in the diversity panel, demonstrating a wide range in iridoid content across tissues. Taken together, this data provides the foundation to dissect the molecular and genetic basis of iridoid production in blueberry.

LanguageEnglish (US)
Article number0179417
JournalPLoS ONE
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2017

Profile

Iridoids
Blueberry Plants
blueberries
Fruits
iridoids
Iridoid Glycosides
Tissue
Vaccinium virgatum
Vaccinium angustifolium
Biosynthesis
Vaccinium corymbosum
Bioactivity
Ericaceae
Crops
Anti-Inflammatory Agents
Antioxidants
Vaccinium darrowii
Vaccinium
Fruit
bilberries

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Differential iridoid production as revealed by a diversity panel of 84 cultivated and wild blueberry species. / Leisner, Courtney P.; Kamileen, Mohamed O.; Conway, Megan E.; O'Connor, Sarah E.; Buell, C. Robin.

In: PLoS ONE, Vol. 12, No. 6, 0179417, 01.06.2017.

Research output: Contribution to journalArticle

Leisner, Courtney P. ; Kamileen, Mohamed O. ; Conway, Megan E. ; O'Connor, Sarah E. ; Buell, C. Robin. / Differential iridoid production as revealed by a diversity panel of 84 cultivated and wild blueberry species. In: PLoS ONE. 2017 ; Vol. 12, No. 6.
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