Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles

Amy M. Baetsen-Young, Matthew Vasher, Leann L. Matta, Phil Colgan, Evangelyn C. Alocilja, Brad Day

Research output: Research - peer-reviewArticle

Abstract

The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection. We demonstrate that d-AuNPs are stable in a five-fold greater salt concentration than citrate-capped AuNPs and the d-AuNPs were stabilized by single stranded DNA probe (ssDNAp). However, in the elevated salt concentrations of the DNA detection assay, the target reactions were surprisingly further stabilized by the formation of a ssDNAp-target gDNA complex. The results presented herein lead us to propose a mechanism whereby genomic ssDNA secondary structure formation during ssDNAp-to-target gDNA binding enables d-AuNP stabilization in elevated ionic environments. Using the assay described herein, we were successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pathogen matrix, as few as 18 spores/µL.

LanguageEnglish (US)
Pages29-36
Number of pages8
JournalBiosensors and Bioelectronics
Volume101
DOIs
StatePublished - Mar 15 2018

Profile

Pathogens
Gold
Nanoparticles
DNA
caloreen
Single-Stranded DNA
DNA Probes
Salts
Assays
Spores
Citric Acid
Nucleic Acids
Nanosensors
Agglomeration
Stabilization
Nucleic acids

Keywords

  • Dextrin gold nanoparticles
  • DNA
  • Genomic
  • Pathogen
  • Salt
  • Stabilization

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles. / Baetsen-Young, Amy M.; Vasher, Matthew; Matta, Leann L.; Colgan, Phil; Alocilja, Evangelyn C.; Day, Brad.

In: Biosensors and Bioelectronics, Vol. 101, 15.03.2018, p. 29-36.

Research output: Research - peer-reviewArticle

Baetsen-Young, Amy M. ; Vasher, Matthew ; Matta, Leann L. ; Colgan, Phil ; Alocilja, Evangelyn C. ; Day, Brad. / Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles. In: Biosensors and Bioelectronics. 2018 ; Vol. 101. pp. 29-36
@article{68cf65f9b2354844bbebaea911dcdac8,
title = "Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles",
abstract = "The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection. We demonstrate that d-AuNPs are stable in a five-fold greater salt concentration than citrate-capped AuNPs and the d-AuNPs were stabilized by single stranded DNA probe (ssDNAp). However, in the elevated salt concentrations of the DNA detection assay, the target reactions were surprisingly further stabilized by the formation of a ssDNAp-target gDNA complex. The results presented herein lead us to propose a mechanism whereby genomic ssDNA secondary structure formation during ssDNAp-to-target gDNA binding enables d-AuNP stabilization in elevated ionic environments. Using the assay described herein, we were successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pathogen matrix, as few as 18 spores/µL.",
keywords = "Dextrin gold nanoparticles, DNA, Genomic, Pathogen, Salt, Stabilization",
author = "Baetsen-Young, {Amy M.} and Matthew Vasher and Matta, {Leann L.} and Phil Colgan and Alocilja, {Evangelyn C.} and Brad Day",
year = "2018",
month = "3",
doi = "10.1016/j.bios.2017.10.011",
volume = "101",
pages = "29--36",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",

}

TY - JOUR

T1 - Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles

AU - Baetsen-Young,Amy M.

AU - Vasher,Matthew

AU - Matta,Leann L.

AU - Colgan,Phil

AU - Alocilja,Evangelyn C.

AU - Day,Brad

PY - 2018/3/15

Y1 - 2018/3/15

N2 - The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection. We demonstrate that d-AuNPs are stable in a five-fold greater salt concentration than citrate-capped AuNPs and the d-AuNPs were stabilized by single stranded DNA probe (ssDNAp). However, in the elevated salt concentrations of the DNA detection assay, the target reactions were surprisingly further stabilized by the formation of a ssDNAp-target gDNA complex. The results presented herein lead us to propose a mechanism whereby genomic ssDNA secondary structure formation during ssDNAp-to-target gDNA binding enables d-AuNP stabilization in elevated ionic environments. Using the assay described herein, we were successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pathogen matrix, as few as 18 spores/µL.

AB - The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection. We demonstrate that d-AuNPs are stable in a five-fold greater salt concentration than citrate-capped AuNPs and the d-AuNPs were stabilized by single stranded DNA probe (ssDNAp). However, in the elevated salt concentrations of the DNA detection assay, the target reactions were surprisingly further stabilized by the formation of a ssDNAp-target gDNA complex. The results presented herein lead us to propose a mechanism whereby genomic ssDNA secondary structure formation during ssDNAp-to-target gDNA binding enables d-AuNP stabilization in elevated ionic environments. Using the assay described herein, we were successful in detecting as little as 2.94 fM of pathogen DNA, and using crude extractions of a pathogen matrix, as few as 18 spores/µL.

KW - Dextrin gold nanoparticles

KW - DNA

KW - Genomic

KW - Pathogen

KW - Salt

KW - Stabilization

UR - http://www.scopus.com/inward/record.url?scp=85033556407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033556407&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2017.10.011

DO - 10.1016/j.bios.2017.10.011

M3 - Article

VL - 101

SP - 29

EP - 36

JO - Biosensors and Bioelectronics

T2 - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -