Direct transfer of preformed patterned bio-nanocomposite films on polyelectrolyte multilayer templates

Research output: Contribution to journalArticle

  • 11 Citations

Abstract

Microarrays containing multiple, nanostructured layers of biological materials would enable high-throughput screening of drug candidates, investigation of protein-mediated cell adhesion, and fabrication of novel biosensors. In this paper, we have examined in detail an approach that allows high-quality microarrays of layered, bionanocomposite films to be deposited on virtually any substrate. The approach uses LBL self-assembly to pre-establish a multi-layered structure on an elastomeric stamp, and then uses μCP to transfer the 3-D structure intact to the target surface. For examples, different 3-D patterns containing dendrimers, polyelectrolyte multilayers and two proteins, sADH and sDH, have been fabricated. For the first time, the approach was also extended to create overlaid bionanocomposite patterns and multiple proteins containing patterns. The approach overcomes a problem encountered when using μCP to establish a pattern on the target surface and then building sequential layers on the pattern via LBL self-assembly. Amphiphilic molecules such as proteins and dendrimers tend to adsorb both to the patterned features as well as the underlying substrate, resulting in low-quality patterns. By circumventing this problem, this research significantly extends the range of surfaces and layering constituents that can be used to fabricate 3-D, patterned, bionanocomposite structures.

LanguageEnglish (US)
Pages789-797
Number of pages9
JournalMacromolecular Bioscience
Volume7
Issue number6
DOIs
StatePublished - Jun 7 2007

Profile

Nanocomposites
Nanocomposite films
Polyelectrolytes
Multilayers
Proteins
Dendrimers
Microarrays
Self assembly
Preclinical Drug Evaluations
Cell adhesion
Biosensing Techniques
Substrates
Biosensors
Cell Adhesion
Biological materials
Screening
Throughput
Fabrication
Molecules
Research

Keywords

  • Atomic force microscopy (AFM)
  • Bionanocomposite array
  • Dendrimers
  • Fluorescence microscopy
  • Intact transfer printing
  • ITP
  • Micro-contact printing
  • Microarrays
  • Multilayers
  • Polyelectrolytes
  • Proteins
  • Self-assembly

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry
  • Bioengineering

Cite this

Direct transfer of preformed patterned bio-nanocomposite films on polyelectrolyte multilayer templates. / Kohli, Neeraj; Worden, Robert M.; Lee, Ilsoon.

In: Macromolecular Bioscience, Vol. 7, No. 6, 07.06.2007, p. 789-797.

Research output: Contribution to journalArticle

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