Discovery of a filamentous chaperone from a hyperthermophile: Molecular function and potential applications

Timothy Whitehead, Boonchai B. Boonyaratanakornkit, Douglas S. Clark

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Many molecular chaperones are oligomeric complexes comprising 14 or more subunits. However, most assemble in a globular manner. We report the discovery of a hyperthermophilic molecular chaperone, a prefoldin from Methanococcus jannaschii, which assembles as a filament. To our knowledge, this is the first known functional filamentous chaperone. Dimensions of the chaperone are quantified by size exclusion chromatography, atomic force microscopy, and transmission electron microscopy measurements. The filaments show remarkable stability against common denaturants, temperature, and salts. Further, it is functional as a chaperone in vitro. Comparisons to the α/β prefoldin are shown, and differences highlighted. In light of the recently solved crystal structure of α/β prefoldin from a thermophile, we present a molecular model for filament assembly. Potential applications for bioprocessing will be discussed.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages8392
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Profile

Size exclusion chromatography
Atomic force microscopy
Crystal structure
Salts
Transmission electron microscopy
Temperature

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Whitehead, T., Boonyaratanakornkit, B. B., & Clark, D. S. (2005). Discovery of a filamentous chaperone from a hyperthermophile: Molecular function and potential applications. In AIChE Annual Meeting, Conference Proceedings (pp. 8392)

Discovery of a filamentous chaperone from a hyperthermophile : Molecular function and potential applications. / Whitehead, Timothy; Boonyaratanakornkit, Boonchai B.; Clark, Douglas S.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 8392.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Whitehead, T, Boonyaratanakornkit, BB & Clark, DS 2005, Discovery of a filamentous chaperone from a hyperthermophile: Molecular function and potential applications. in AIChE Annual Meeting, Conference Proceedings. pp. 8392, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 30-4 November.
Whitehead T, Boonyaratanakornkit BB, Clark DS. Discovery of a filamentous chaperone from a hyperthermophile: Molecular function and potential applications. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 8392.

Whitehead, Timothy; Boonyaratanakornkit, Boonchai B.; Clark, Douglas S. / Discovery of a filamentous chaperone from a hyperthermophile : Molecular function and potential applications.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 8392.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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