Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography

Eric R. Swy, Aaron S. Schwartz-Duval, Dorela D. Shuboni, Matthew T. Latourette, Christiane L. Mallet, Maciej Parys, David P. Cormode, Erik M. Shapiro

    Research output: Research - peer-reviewArticle

    • 13 Citations

    Abstract

    Reports of molecular and cellular imaging using computed tomography (CT) are rapidly increasing. Many of these reports use gold nanoparticles. Bismuth has similar CT contrast properties to gold while being approximately 1000-fold less expensive. Herein we report the design, fabrication, characterization, and CT and fluorescence imaging properties of a novel, dual modality, fluorescent, polymer encapsulated bismuth nanoparticle construct for computed tomography and fluorescence imaging. We also report on cellular internalization and preliminary in vitro and in vivo toxicity effects of these constructs. 40 nm bismuth(0) nanocrystals were synthesized and encapsulated within 120 nm Poly(dl-lactic-co-glycolic acid) (PLGA) nanoparticles by oil-in-water emulsion methodologies. Coumarin-6 was co-encapsulated to impart fluorescence. High encapsulation efficiency was achieved ∼70% bismuth w/w. Particles were shown to internalize within cells following incubation in culture. Bismuth nanocrystals and PLGA encapsulated bismuth nanoparticles exhibited >90% and >70% degradation, respectively, within 24 hours in acidic, lysosomal environment mimicking media and both remained nearly 100% stable in cytosolic/extracellular fluid mimicking media. μCT and clinical CT imaging was performed at multiple X-ray tube voltages to measure concentration dependent attenuation rates as well as to establish the ability to detect the nanoparticles in an ex vivo biological sample. Dual fluorescence and CT imaging is demonstrated as well. In vivo toxicity studies in rats revealed neither clinically apparent side effects nor major alterations in serum chemistry and hematology parameters. Calculations on minimal detection requirements for in vivo targeted imaging using these nanoparticles are presented. Indeed, our results indicate that these nanoparticles may serve as a platform for sensitive and specific targeted molecular CT and fluorescence imaging.

    LanguageEnglish (US)
    Pages13104-13112
    Number of pages9
    JournalNanoscale
    Volume6
    Issue number21
    DOIs
    StatePublished - Nov 7 2014

    Profile

    glycolic acid
    Bismuth
    Tomography
    Fluorescence
    Nanoparticles
    Imaging techniques
    Milk
    Acids
    Gold
    Nanocrystals
    Toxicity
    X ray tubes
    Emulsions
    Encapsulation
    Rats
    Polymers
    Oils
    Fabrication
    Degradation
    Fluids

    ASJC Scopus subject areas

    • Materials Science(all)

    Cite this

    Swy, E. R., Schwartz-Duval, A. S., Shuboni, D. D., Latourette, M. T., Mallet, C. L., Parys, M., ... Shapiro, E. M. (2014). Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography. Nanoscale, 6(21), 13104-13112. DOI: 10.1039/c4nr01405g

    Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography. / Swy, Eric R.; Schwartz-Duval, Aaron S.; Shuboni, Dorela D.; Latourette, Matthew T.; Mallet, Christiane L.; Parys, Maciej; Cormode, David P.; Shapiro, Erik M.

    In: Nanoscale, Vol. 6, No. 21, 07.11.2014, p. 13104-13112.

    Research output: Research - peer-reviewArticle

    Swy, ER, Schwartz-Duval, AS, Shuboni, DD, Latourette, MT, Mallet, CL, Parys, M, Cormode, DP & Shapiro, EM 2014, 'Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography' Nanoscale, vol 6, no. 21, pp. 13104-13112. DOI: 10.1039/c4nr01405g
    Swy ER, Schwartz-Duval AS, Shuboni DD, Latourette MT, Mallet CL, Parys M et al. Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography. Nanoscale. 2014 Nov 7;6(21):13104-13112. Available from, DOI: 10.1039/c4nr01405g
    Swy, Eric R. ; Schwartz-Duval, Aaron S. ; Shuboni, Dorela D. ; Latourette, Matthew T. ; Mallet, Christiane L. ; Parys, Maciej ; Cormode, David P. ; Shapiro, Erik M./ Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular and cellular fluorescence imaging and computed tomography. In: Nanoscale. 2014 ; Vol. 6, No. 21. pp. 13104-13112
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