Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli

Christian Andersson, David Hodge, Kris A. Berglund, Ulrika Rova

Research output: Contribution to journalArticle

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Abstract

Succinic acid (SA) is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present work, dual-phase batch fermentations with the E. coli strain AFP184 were performed using a medium suited for large-scale industrial production of SA. The ability of the strain to ferment different sugars was investigated. The sugars studied were sucrose, glucose, fructose, xylose, and equal mixtures of glucose and fructose and glucose and xylose at a total initial sugar concentration of 100 g L -1. AFP184 was able to utilize all sugars and sugar combinations except sucrose for biomass generation and succinate production. For sucrose as a substrate no succinic acid was produced and none of the sucrose was metabolized. The succinic acid yield from glucose (0.83 g succinic acid per gram glucose consumed anaerobically) was higher than the yield from fructose (0.66 g g-1). When using xylose as a carbon source, a yield of 0.50 g g -1 was obtained. In the mixed-sugar fermentations no catabolite repression was detected. Mixtures of glucose and xylose resulted in higher yields (0.60 g g-1) than use of xylose alone. Fermenting glucose mixed with fructose gave a lower yield (0.58 g g-1) than fructose used as the sole carbon source. The reason is an increased pyruvate production. The pyruvate concentration decreased later in the fermentation. Final succinic acid concentrations were in the range of 25-40 g L-1. Acetic and pyruvic acid were the only other products detected and accumulated to concentrations of 2.7-6.7 and 0-2.7 g L-1. Production of succinic acid decreased when organic acid concentrations reached approximately 30 g L-1. This study demonstrates that E. coli strain AFP184 is able to produce succinic acid in a low cost medium from a variety of sugars with only small amounts of byproducts formed.

Original languageEnglish (US)
Pages (from-to)381-388
Number of pages8
JournalBiotechnology Progress
Volume23
Issue number2
DOIs
StatePublished - Mar 2007
Externally publishedYes

Profile

Succinic Acid
Carbon
Escherichia coli
succinic acid
Carbohydrates
Glucose
sugars
glucose
Xylose
Fructose
xylose
fructose
Sucrose
sucrose
Fermentation
carbon
Pyruvic Acid
fermentation
Catabolite Repression
Acetic Acid

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli. / Andersson, Christian; Hodge, David; Berglund, Kris A.; Rova, Ulrika.

In: Biotechnology Progress, Vol. 23, No. 2, 03.2007, p. 381-388.

Research output: Contribution to journalArticle

Andersson, Christian; Hodge, David; Berglund, Kris A.; Rova, Ulrika / Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli.

In: Biotechnology Progress, Vol. 23, No. 2, 03.2007, p. 381-388.

Research output: Contribution to journalArticle

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