Effect of heat on phycoerythrin fluorescence: Influence of thermal exposure on the fluorescence emission of R-phycoerythrin

S. Vaidya, A. Orta-Ramirez, D. M. Smith, Robert Y. Ofoli

    Research output: Contribution to journalArticle

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    Abstract

    The goal of this work was to measure and model the effect of thermal exposure on the fluorescence emission of R-phycoerythrin (R-PE). The long-term objective of our work is to assess the feasibility of encapsulating R-PE for use as the critical component of a time-temperature integrator (TTI) for ascertaining the degree of inactivation of food pathogens such as Salmonella. In this article we present a study to measure and model the thermally induced fluorescence emission decay of R-PE in several isothermal experiments. We used the isothermal data to determine the kinetic parameters, based on a general nth order reaction, and evaluated the utility of the resulting model by using it to predict R-PE fluorescence emission decay for several nonisothermal experiments based on published USDA safe harbor guidelines for cooked beef products. The transient experiments were conducted over the same temperature range used in the isothermal study. Very good agreement was obtained between theory and experiment at temperatures of 62.8°C and above, although the model slightly underpredicted the extent of fluorescence emission decay at 60°C. Our results indicate that R-PE fluorescence emission decay kinetics is well behaved and that the protein is a strong candidate for use as a time-temperature integrator.

    Original languageEnglish (US)
    Pages (from-to)465-473
    Number of pages9
    JournalBiotechnology and Bioengineering
    Volume83
    Issue number4
    DOIs
    StatePublished - Aug 20 2003

    Profile

    Phycoerythrin
    Hot Temperature
    Fluorescence
    Temperature
    Common Bile Duct Diseases
    Acetanilides
    Anthralin
    Experiments
    United States Department of Agriculture
    Salmonella
    Food
    Proteins
    State Dentistry
    Beef
    Genetic Engineering
    Pathogens
    Ports and harbors
    Kinetic parameters
    Kinetics

    Keywords

    • Fluorescence emission decay
    • Food safety
    • Protein-based sensor
    • Reaction kinetics
    • Time-temperature history

    ASJC Scopus subject areas

    • Biotechnology
    • Microbiology

    Cite this

    Effect of heat on phycoerythrin fluorescence : Influence of thermal exposure on the fluorescence emission of R-phycoerythrin. / Vaidya, S.; Orta-Ramirez, A.; Smith, D. M.; Ofoli, Robert Y.

    In: Biotechnology and Bioengineering, Vol. 83, No. 4, 20.08.2003, p. 465-473.

    Research output: Contribution to journalArticle

    Vaidya, S.; Orta-Ramirez, A.; Smith, D. M.; Ofoli, Robert Y. / Effect of heat on phycoerythrin fluorescence : Influence of thermal exposure on the fluorescence emission of R-phycoerythrin.

    In: Biotechnology and Bioengineering, Vol. 83, No. 4, 20.08.2003, p. 465-473.

    Research output: Contribution to journalArticle

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