Effect of hydrogen bonding on the rotational and translational dynamics of a headgroup-bound chromophore in bilayer lipid membranes

Aaron J. Greiner, Heather A. Pillman, R. M. Worden, G. J. Blanchard, R. Y. Ofoli

Research output: Research - peer-reviewArticle

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Abstract

We have studied the interactions of the chromophore l,2-dioleoyl-OT- glycero-3-phosphoethanolamine-N-7- nitro-2-l,3-benzoxadiazol-4-yl (18:1 NBD-PE) imbedded in the headgroup region of bilayer lipid membranes consisting of l,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and l,2-dioleoyl-sn-glycero-3- [phospho-rac-(1-glycerol) ] (DOPG). We have examined the molecular and mesoscale dynamics of the chromophore using time-correlated single photon counting (TCSPC) to measure rotational diffusion dynamics in lipid vesicles and fluorescence recovery after pattern photobleaching (FRAPP) to determine translational diffusion coefficients and mobile fractions in supported lipid bilayers. TCSPC data reveal that chromophore rotational diffusion rates in DOPG vesicles are statistically the same as in DOPC and mixed DOPC/DOPG vesicles, suggesting that the NBD-PE chromophore does not interact strongly with the headgroup region of these bilayers; however, FRAPP experiments show that lateral diffusion is statistically lower in mixed DOPC/DOPG-supported bilayers than in DOPC-supported bilayers. These results suggest that bilayers containing DOPG likely undergo interlipid headgroup hydrogen bonding interactions that suppress translational diffusion.

LanguageEnglish (US)
Pages13263-13268
Number of pages6
JournalJournal of Physical Chemistry B
Volume113
Issue number40
DOIs
StatePublished - Oct 8 2009

Profile

Lipid bilayers
Phosphorylcholine
Chromophores
Membrane Lipids
Hydrogen bonds
1,2-dioleoyl-sn-glycero-3-phosphoglycerol
chromophores
lipids
membranes
hydrogen
counting
recovery
fluorescence
photons
interactions
Photobleaching
Photons
Fluorescence
Recovery
N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Effect of hydrogen bonding on the rotational and translational dynamics of a headgroup-bound chromophore in bilayer lipid membranes. / Greiner, Aaron J.; Pillman, Heather A.; Worden, R. M.; Blanchard, G. J.; Ofoli, R. Y.

In: Journal of Physical Chemistry B, Vol. 113, No. 40, 08.10.2009, p. 13263-13268.

Research output: Research - peer-reviewArticle

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