Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells

C. Liu, S. Baek, J. Kim, E. Vasko, R. Pyne, C. Chan

    Research output: Contribution to journalArticle

    • 7 Citations

    Abstract

    Mechanical cues in the cellular environment play important roles in guiding various cell behaviors, such as cell alignment, migration, and differentiation. Previous studies investigated mechanical stretch guided cell alignment predominantly with cyclic stretching whereby an external force is applied to stretch the substrate dynamically (i.e., cyclically) while the cells are attached onto the substrate. In contrast, we created a static pre-stretched anisotropic surface in which the cells were seeded subsequent to stretching the substrate. We hypothesized that the cell senses the physical environment through a more active mechanism, namely, even without external forces the cell can actively apply traction and sense an increased stiffness in the stretched direction and align in that direction. To test our hypothesis, we quantified the extent of pre-stretch induced anisotropy by employing the theory of small deformation superimposed on large and predicted the effective stiffness in the stretch direction as well as its perpendicular direction. We showed mesenchymal stem cells (MSC) aligned in the pre-stretched direction, and the cell alignment and morphology were dependent on the prestretch magnitude. In addition, the pre-stretched surface demonstrated an ability to promote early myoblast differentiation of the MSC. This study is the first report on MSC alignment on a statically pre-stretched surface. The cell orientation induced by the pre-stretch induced anisotropy could provide insight into tissue engineering applications involving cells that aligned in vivo in the absence of dynamic mechanical stimuli.

    Original languageEnglish (US)
    Pages (from-to)106-121
    Number of pages16
    JournalCellular and Molecular Bioengineering
    Volume7
    Issue number1
    DOIs
    StatePublished - 2014

    Profile

    Stem cells
    Cell
    Anisotropy
    Mesenchymal Stromal Cells
    Edema Disease of Swine
    Antitubercular Agents
    Status Epilepticus
    Substrates
    Automobiles
    Stretch
    Stretching
    Stiffness
    Diatrizoate
    Alignment
    Substrate
    Traction (friction)
    Tissue engineering
    Myoclonic Cerebellar Dyssynergia
    Carbamyl Phosphate
    Spontaneous Fractures

    Keywords

    • Anisotropy
    • Mechano-sensing
    • Mesenchymal stem cells
    • Orientation
    • Static pre-stretch

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Modeling and Simulation

    Cite this

    Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells. / Liu, C.; Baek, S.; Kim, J.; Vasko, E.; Pyne, R.; Chan, C.

    In: Cellular and Molecular Bioengineering, Vol. 7, No. 1, 2014, p. 106-121.

    Research output: Contribution to journalArticle

    Liu, C.; Baek, S.; Kim, J.; Vasko, E.; Pyne, R.; Chan, C. / Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells.

    In: Cellular and Molecular Bioengineering, Vol. 7, No. 1, 2014, p. 106-121.

    Research output: Contribution to journalArticle

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