Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells

C. Liu, S. Baek, J. Kim, E. Vasko, R. Pyne, C. Chan

Research output: Contribution to journalArticle

  • 9 Citations

Abstract

Mechanical cues in the cellular environment play important roles in guiding various cell behaviors, such as cell alignment, migration, and differentiation. Previous studies investigated mechanical stretch guided cell alignment predominantly with cyclic stretching whereby an external force is applied to stretch the substrate dynamically (i.e., cyclically) while the cells are attached onto the substrate. In contrast, we created a static pre-stretched anisotropic surface in which the cells were seeded subsequent to stretching the substrate. We hypothesized that the cell senses the physical environment through a more active mechanism, namely, even without external forces the cell can actively apply traction and sense an increased stiffness in the stretched direction and align in that direction. To test our hypothesis, we quantified the extent of pre-stretch induced anisotropy by employing the theory of small deformation superimposed on large and predicted the effective stiffness in the stretch direction as well as its perpendicular direction. We showed mesenchymal stem cells (MSC) aligned in the pre-stretched direction, and the cell alignment and morphology were dependent on the prestretch magnitude. In addition, the pre-stretched surface demonstrated an ability to promote early myoblast differentiation of the MSC. This study is the first report on MSC alignment on a statically pre-stretched surface. The cell orientation induced by the pre-stretch induced anisotropy could provide insight into tissue engineering applications involving cells that aligned in vivo in the absence of dynamic mechanical stimuli.

LanguageEnglish (US)
Pages106-121
Number of pages16
JournalCellular and Molecular Bioengineering
Volume7
Issue number1
DOIs
StatePublished - 2014

Profile

Stem Cells
Anisotropy
Stretch
Stem cells
Mesenchymal Stromal Cells
Cell
Stretching
Substrates
Stiffness
Alignment
Traction (friction)
Tissue engineering
Substrate
Direction compound
Aptitude
Tissue Engineering
Myoblasts
Traction
Hypothesis Test
Engineering Application

Keywords

  • Anisotropy
  • Mechano-sensing
  • Mesenchymal stem cells
  • Orientation
  • Static pre-stretch

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells. / Liu, C.; Baek, S.; Kim, J.; Vasko, E.; Pyne, R.; Chan, C.

In: Cellular and Molecular Bioengineering, Vol. 7, No. 1, 2014, p. 106-121.

Research output: Contribution to journalArticle

@article{4b19b514289c400885957f3e5795884e,
title = "Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells",
abstract = "Mechanical cues in the cellular environment play important roles in guiding various cell behaviors, such as cell alignment, migration, and differentiation. Previous studies investigated mechanical stretch guided cell alignment predominantly with cyclic stretching whereby an external force is applied to stretch the substrate dynamically (i.e., cyclically) while the cells are attached onto the substrate. In contrast, we created a static pre-stretched anisotropic surface in which the cells were seeded subsequent to stretching the substrate. We hypothesized that the cell senses the physical environment through a more active mechanism, namely, even without external forces the cell can actively apply traction and sense an increased stiffness in the stretched direction and align in that direction. To test our hypothesis, we quantified the extent of pre-stretch induced anisotropy by employing the theory of small deformation superimposed on large and predicted the effective stiffness in the stretch direction as well as its perpendicular direction. We showed mesenchymal stem cells (MSC) aligned in the pre-stretched direction, and the cell alignment and morphology were dependent on the prestretch magnitude. In addition, the pre-stretched surface demonstrated an ability to promote early myoblast differentiation of the MSC. This study is the first report on MSC alignment on a statically pre-stretched surface. The cell orientation induced by the pre-stretch induced anisotropy could provide insight into tissue engineering applications involving cells that aligned in vivo in the absence of dynamic mechanical stimuli.",
keywords = "Anisotropy, Mechano-sensing, Mesenchymal stem cells, Orientation, Static pre-stretch",
author = "C. Liu and S. Baek and J. Kim and E. Vasko and R. Pyne and C. Chan",
year = "2014",
doi = "10.1007/s12195-013-0300-0",
language = "English (US)",
volume = "7",
pages = "106--121",
journal = "Cellular and Molecular Bioengineering",
issn = "1865-5025",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Effect of static pre-stretch induced surface anisotropy on orientation of mesenchymal stem cells

AU - Liu,C.

AU - Baek,S.

AU - Kim,J.

AU - Vasko,E.

AU - Pyne,R.

AU - Chan,C.

PY - 2014

Y1 - 2014

N2 - Mechanical cues in the cellular environment play important roles in guiding various cell behaviors, such as cell alignment, migration, and differentiation. Previous studies investigated mechanical stretch guided cell alignment predominantly with cyclic stretching whereby an external force is applied to stretch the substrate dynamically (i.e., cyclically) while the cells are attached onto the substrate. In contrast, we created a static pre-stretched anisotropic surface in which the cells were seeded subsequent to stretching the substrate. We hypothesized that the cell senses the physical environment through a more active mechanism, namely, even without external forces the cell can actively apply traction and sense an increased stiffness in the stretched direction and align in that direction. To test our hypothesis, we quantified the extent of pre-stretch induced anisotropy by employing the theory of small deformation superimposed on large and predicted the effective stiffness in the stretch direction as well as its perpendicular direction. We showed mesenchymal stem cells (MSC) aligned in the pre-stretched direction, and the cell alignment and morphology were dependent on the prestretch magnitude. In addition, the pre-stretched surface demonstrated an ability to promote early myoblast differentiation of the MSC. This study is the first report on MSC alignment on a statically pre-stretched surface. The cell orientation induced by the pre-stretch induced anisotropy could provide insight into tissue engineering applications involving cells that aligned in vivo in the absence of dynamic mechanical stimuli.

AB - Mechanical cues in the cellular environment play important roles in guiding various cell behaviors, such as cell alignment, migration, and differentiation. Previous studies investigated mechanical stretch guided cell alignment predominantly with cyclic stretching whereby an external force is applied to stretch the substrate dynamically (i.e., cyclically) while the cells are attached onto the substrate. In contrast, we created a static pre-stretched anisotropic surface in which the cells were seeded subsequent to stretching the substrate. We hypothesized that the cell senses the physical environment through a more active mechanism, namely, even without external forces the cell can actively apply traction and sense an increased stiffness in the stretched direction and align in that direction. To test our hypothesis, we quantified the extent of pre-stretch induced anisotropy by employing the theory of small deformation superimposed on large and predicted the effective stiffness in the stretch direction as well as its perpendicular direction. We showed mesenchymal stem cells (MSC) aligned in the pre-stretched direction, and the cell alignment and morphology were dependent on the prestretch magnitude. In addition, the pre-stretched surface demonstrated an ability to promote early myoblast differentiation of the MSC. This study is the first report on MSC alignment on a statically pre-stretched surface. The cell orientation induced by the pre-stretch induced anisotropy could provide insight into tissue engineering applications involving cells that aligned in vivo in the absence of dynamic mechanical stimuli.

KW - Anisotropy

KW - Mechano-sensing

KW - Mesenchymal stem cells

KW - Orientation

KW - Static pre-stretch

UR - http://www.scopus.com/inward/record.url?scp=84899477612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899477612&partnerID=8YFLogxK

U2 - 10.1007/s12195-013-0300-0

DO - 10.1007/s12195-013-0300-0

M3 - Article

VL - 7

SP - 106

EP - 121

JO - Cellular and Molecular Bioengineering

T2 - Cellular and Molecular Bioengineering

JF - Cellular and Molecular Bioengineering

SN - 1865-5025

IS - 1

ER -