Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Mingjie Jin, William Bothfeld, Samantha Austin, Trey K. Sato, Alex La Reau, Haibo Li, Marcus Foston, Christa Gunawan, Richard D. LeDuc, John F. Quensen, Mick Mcgee, Nirmal Uppugundla, Alan Higbee, Ruwan Ranatunga, Charles W. Donald, Gwen Bone, Arthur J. Ragauskas, James M. Tiedje, Daniel R. Noguera, Bruce E. Dale & 2 others Yaoping Zhang, Venkatesh Balan

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.

LanguageEnglish (US)
Pages212-220
Number of pages9
JournalBioresource Technology
Volume147
DOIs
StatePublished - Nov 2013

Profile

Precipitates
Yeast
Fermentation
Trademarks
Cell growth
Heavy Metals
Chemical analysis
Ammonia
Sugars
Escherichia coli
Heavy metals
fermentation
Minerals
Assays
Nuclear magnetic resonance
struvite
Fibers
yeast
nuclear magnetic resonance
effect

Keywords

  • AFEX
  • Hydrolysate characterization
  • Hydrolysate fermentation
  • Hydrolysate precipitate
  • Hydrolysate storage

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Jin, M., Bothfeld, W., Austin, S., Sato, T. K., La Reau, A., Li, H., ... Balan, V. (2013). Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. Bioresource Technology, 147, 212-220. DOI: 10.1016/j.biortech.2013.08.018

Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. / Jin, Mingjie; Bothfeld, William; Austin, Samantha; Sato, Trey K.; La Reau, Alex; Li, Haibo; Foston, Marcus; Gunawan, Christa; LeDuc, Richard D.; Quensen, John F.; Mcgee, Mick; Uppugundla, Nirmal; Higbee, Alan; Ranatunga, Ruwan; Donald, Charles W.; Bone, Gwen; Ragauskas, Arthur J.; Tiedje, James M.; Noguera, Daniel R.; Dale, Bruce E.; Zhang, Yaoping; Balan, Venkatesh.

In: Bioresource Technology, Vol. 147, 11.2013, p. 212-220.

Research output: Contribution to journalArticle

Jin, M, Bothfeld, W, Austin, S, Sato, TK, La Reau, A, Li, H, Foston, M, Gunawan, C, LeDuc, RD, Quensen, JF, Mcgee, M, Uppugundla, N, Higbee, A, Ranatunga, R, Donald, CW, Bone, G, Ragauskas, AJ, Tiedje, JM, Noguera, DR, Dale, BE, Zhang, Y & Balan, V 2013, 'Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate' Bioresource Technology, vol 147, pp. 212-220. DOI: 10.1016/j.biortech.2013.08.018
Jin M, Bothfeld W, Austin S, Sato TK, La Reau A, Li H et al. Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. Bioresource Technology. 2013 Nov;147:212-220. Available from, DOI: 10.1016/j.biortech.2013.08.018
Jin, Mingjie ; Bothfeld, William ; Austin, Samantha ; Sato, Trey K. ; La Reau, Alex ; Li, Haibo ; Foston, Marcus ; Gunawan, Christa ; LeDuc, Richard D. ; Quensen, John F. ; Mcgee, Mick ; Uppugundla, Nirmal ; Higbee, Alan ; Ranatunga, Ruwan ; Donald, Charles W. ; Bone, Gwen ; Ragauskas, Arthur J. ; Tiedje, James M. ; Noguera, Daniel R. ; Dale, Bruce E. ; Zhang, Yaoping ; Balan, Venkatesh. / Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. In: Bioresource Technology. 2013 ; Vol. 147. pp. 212-220
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