Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

Mingjie Jin, William Bothfeld, Samantha Austin, Trey K. Sato, Alex La Reau, Haibo Li, Marcus Foston, Christa Gunawan, Richard D. LeDuc, John F. Quensen, Mick Mcgee, Nirmal Uppugundla, Alan Higbee, Ruwan Ranatunga, Charles W. Donald, Gwen Bone, Arthur J. Ragauskas, James M. Tiedje, Daniel R. Noguera, Bruce E. Dale & 2 others Yaoping Zhang, Venkatesh Balan

    Research output: Contribution to journalArticle

    • 7 Citations

    Abstract

    To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.

    Original languageEnglish (US)
    Pages (from-to)212-220
    Number of pages9
    JournalBioresource Technology
    Volume147
    DOIs
    StatePublished - Nov 2013

    Profile

    Precipitates
    Yeast
    Fermentation
    Chemical analysis
    African horse sickness virus
    HLA Antigens
    Trademarks
    Cell growth
    Sugars
    Escherichia coli
    Heavy metals
    Ammonia
    Assays
    Minerals
    Nuclear magnetic resonance
    Fibers
    Temperature
    Frangula
    Acetanilides
    Bibliography of Medicine

    Keywords

    • AFEX
    • Hydrolysate characterization
    • Hydrolysate fermentation
    • Hydrolysate precipitate
    • Hydrolysate storage

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal

    Cite this

    Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. / Jin, Mingjie; Bothfeld, William; Austin, Samantha; Sato, Trey K.; La Reau, Alex; Li, Haibo; Foston, Marcus; Gunawan, Christa; LeDuc, Richard D.; Quensen, John F.; Mcgee, Mick; Uppugundla, Nirmal; Higbee, Alan; Ranatunga, Ruwan; Donald, Charles W.; Bone, Gwen; Ragauskas, Arthur J.; Tiedje, James M.; Noguera, Daniel R.; Dale, Bruce E.; Zhang, Yaoping; Balan, Venkatesh.

    In: Bioresource Technology, Vol. 147, 11.2013, p. 212-220.

    Research output: Contribution to journalArticle

    Jin, M, Bothfeld, W, Austin, S, Sato, TK, La Reau, A, Li, H, Foston, M, Gunawan, C, LeDuc, RD, Quensen, JF, Mcgee, M, Uppugundla, N, Higbee, A, Ranatunga, R, Donald, CW, Bone, G, Ragauskas, AJ, Tiedje, JM, Noguera, DR, Dale, BE, Zhang, Y & Balan, V 2013, 'Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate' Bioresource Technology, vol 147, pp. 212-220. DOI: 10.1016/j.biortech.2013.08.018
    Jin M, Bothfeld W, Austin S, Sato TK, La Reau A, Li H et al. Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. Bioresource Technology. 2013 Nov;147:212-220. Available from, DOI: 10.1016/j.biortech.2013.08.018

    Jin, Mingjie; Bothfeld, William; Austin, Samantha; Sato, Trey K.; La Reau, Alex; Li, Haibo; Foston, Marcus; Gunawan, Christa; LeDuc, Richard D.; Quensen, John F.; Mcgee, Mick; Uppugundla, Nirmal; Higbee, Alan; Ranatunga, Ruwan; Donald, Charles W.; Bone, Gwen; Ragauskas, Arthur J.; Tiedje, James M.; Noguera, Daniel R.; Dale, Bruce E.; Zhang, Yaoping; Balan, Venkatesh / Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate.

    In: Bioresource Technology, Vol. 147, 11.2013, p. 212-220.

    Research output: Contribution to journalArticle

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    AU - Bothfeld,William

    AU - Austin,Samantha

    AU - Sato,Trey K.

    AU - La Reau,Alex

    AU - Li,Haibo

    AU - Foston,Marcus

    AU - Gunawan,Christa

    AU - LeDuc,Richard D.

    AU - Quensen,John F.

    AU - Mcgee,Mick

    AU - Uppugundla,Nirmal

    AU - Higbee,Alan

    AU - Ranatunga,Ruwan

    AU - Donald,Charles W.

    AU - Bone,Gwen

    AU - Ragauskas,Arthur J.

    AU - Tiedje,James M.

    AU - Noguera,Daniel R.

    AU - Dale,Bruce E.

    AU - Zhang,Yaoping

    AU - Balan,Venkatesh

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