Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen

Peer Mohamed Abdul, Jamaliah Md Jahim, Shuhaida Harun, Masturah Markom, Nabilah Aminah Lutpi, Osman Hassan, Venkatesh Balan, Bruce E. Dale, Mohd Tusirin Mohd Nor

    Research output: Research - peer-reviewArticle

    • 8 Citations

    Abstract

    Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15 g g-1 of OPEFB in the raw untreated OPEFB sample to 0.53 g g-1 of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.

    LanguageEnglish (US)
    Pages200-208
    Number of pages9
    JournalBioresource Technology
    Volume211
    DOIs
    StatePublished - Jul 1 2016

    Profile

    ammonia
    fruit
    oil
    effect
    chemical
    fibre
    Saccharification
    Palm oil
    Fruits
    Ammonia
    Fibers
    sugar
    Sugars
    FTIR spectroscopy
    structural change
    fermentation
    ethanol
    hydrolysis
    metabolite
    acetate

    Keywords

    • Ammonia fibre expansion (AFEX)
    • Biohydrogen
    • Biomass characterisation
    • Oil palm empty fruit bunch (OPEFB) fibre
    • Oil palm residues

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal

    Cite this

    Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen. / Abdul, Peer Mohamed; Jahim, Jamaliah Md; Harun, Shuhaida; Markom, Masturah; Lutpi, Nabilah Aminah; Hassan, Osman; Balan, Venkatesh; Dale, Bruce E.; Mohd Nor, Mohd Tusirin.

    In: Bioresource Technology, Vol. 211, 01.07.2016, p. 200-208.

    Research output: Research - peer-reviewArticle

    Abdul, Peer Mohamed ; Jahim, Jamaliah Md ; Harun, Shuhaida ; Markom, Masturah ; Lutpi, Nabilah Aminah ; Hassan, Osman ; Balan, Venkatesh ; Dale, Bruce E. ; Mohd Nor, Mohd Tusirin. / Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen. In: Bioresource Technology. 2016 ; Vol. 211. pp. 200-208
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    abstract = "Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15 g g-1 of OPEFB in the raw untreated OPEFB sample to 0.53 g g-1 of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.",
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    AU - Harun,Shuhaida

    AU - Markom,Masturah

    AU - Lutpi,Nabilah Aminah

    AU - Hassan,Osman

    AU - Balan,Venkatesh

    AU - Dale,Bruce E.

    AU - Mohd Nor,Mohd Tusirin

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