Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen

Peer Mohamed Abdul, Jamaliah Md Jahim, Shuhaida Harun, Masturah Markom, Nabilah Aminah Lutpi, Osman Hassan, Venkatesh Balan, Bruce E. Dale, Mohd Tusirin Mohd Nor

    Research output: Contribution to journalArticle

    • 8 Citations

    Abstract

    Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15 g g-1 of OPEFB in the raw untreated OPEFB sample to 0.53 g g-1 of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.

    Original languageEnglish (US)
    Pages (from-to)200-208
    Number of pages9
    JournalBioresource Technology
    Volume211
    DOIs
    StatePublished - Jul 1 2016

    Profile

    Palm oil
    Fruits
    fruit
    oil
    Thiamin-Triphosphatase
    Hypocalcemia
    Fibers
    Abnormal Erythrocytes
    Sugars
    sugar
    Ammonia
    ammonia
    Saimiriine herpesvirus 2
    Saccharification
    Enzymatic hydrolysis
    Metabolites
    Fermentation
    Fourier transform infrared spectroscopy
    Bacteria
    Ethanol

    Keywords

    • Ammonia fibre expansion (AFEX)
    • Biohydrogen
    • Biomass characterisation
    • Oil palm empty fruit bunch (OPEFB) fibre
    • Oil palm residues

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal

    Cite this

    Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen. / Abdul, Peer Mohamed; Jahim, Jamaliah Md; Harun, Shuhaida; Markom, Masturah; Lutpi, Nabilah Aminah; Hassan, Osman; Balan, Venkatesh; Dale, Bruce E.; Mohd Nor, Mohd Tusirin.

    In: Bioresource Technology, Vol. 211, 01.07.2016, p. 200-208.

    Research output: Contribution to journalArticle

    Abdul, Peer Mohamed; Jahim, Jamaliah Md; Harun, Shuhaida; Markom, Masturah; Lutpi, Nabilah Aminah; Hassan, Osman; Balan, Venkatesh; Dale, Bruce E.; Mohd Nor, Mohd Tusirin / Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen.

    In: Bioresource Technology, Vol. 211, 01.07.2016, p. 200-208.

    Research output: Contribution to journalArticle

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    abstract = "Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15 g g-1 of OPEFB in the raw untreated OPEFB sample to 0.53 g g-1 of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.",
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    AU - Harun,Shuhaida

    AU - Markom,Masturah

    AU - Lutpi,Nabilah Aminah

    AU - Hassan,Osman

    AU - Balan,Venkatesh

    AU - Dale,Bruce E.

    AU - Mohd Nor,Mohd Tusirin

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