Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen

Peer Mohamed Abdul, Jamaliah Md Jahim, Shuhaida Harun, Masturah Markom, Nabilah Aminah Lutpi, Osman Hassan, Venkatesh Balan, Bruce E. Dale, Mohd Tusirin Mohd Nor

Research output: Contribution to journalArticle

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Abstract

Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15 g g-1 of OPEFB in the raw untreated OPEFB sample to 0.53 g g-1 of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.

LanguageEnglish (US)
Pages200-208
Number of pages9
JournalBioresource Technology
Volume211
DOIs
StatePublished - Jul 1 2016

Profile

Saccharification
Palm oil
Fruits
Ammonia
ammonia
fruit
Fibers
oil
Sugars
sugar
Enzymatic hydrolysis
effect
chemical
fibre
Butyrates
Metabolites
FTIR spectroscopy
structural change
Fermentation
fermentation

Keywords

  • Ammonia fibre expansion (AFEX)
  • Biohydrogen
  • Biomass characterisation
  • Oil palm empty fruit bunch (OPEFB) fibre
  • Oil palm residues

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen. / Abdul, Peer Mohamed; Jahim, Jamaliah Md; Harun, Shuhaida; Markom, Masturah; Lutpi, Nabilah Aminah; Hassan, Osman; Balan, Venkatesh; Dale, Bruce E.; Mohd Nor, Mohd Tusirin.

In: Bioresource Technology, Vol. 211, 01.07.2016, p. 200-208.

Research output: Contribution to journalArticle

Abdul, Peer Mohamed ; Jahim, Jamaliah Md ; Harun, Shuhaida ; Markom, Masturah ; Lutpi, Nabilah Aminah ; Hassan, Osman ; Balan, Venkatesh ; Dale, Bruce E. ; Mohd Nor, Mohd Tusirin. / Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen. In: Bioresource Technology. 2016 ; Vol. 211. pp. 200-208
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AU - Lutpi,Nabilah Aminah

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