Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform

Maria Enquist-Newman, Ann Marie E Faust, Daniel D. Bravo, Christine Nicole S Santos, Ryan M. Raisner, Arthur Hanel, Preethi Sarvabhowman, Chi Le, Drew D. Regitsky, Susan R. Cooper, Lars Peereboom, Alana Clark, Yessica Martinez, Joshua Goldsmith, Min Y. Cho, Paul D. Donohoue, Lily Luo, Brigit Lamberson, Pramila Tamrakar, Edward J. Kim & 12 others Jeffrey L. Villari, Avinash Gill, Shital A. Tripathi, Padma Karamchedu, Carlos J. Paredes, Vineet Rajgarhia, Hans Kristian Kotlar, Richard B. Bailey, Dennis J. Miller, Nicholas L. Ohler, Candace Swimmer, Yasuo Yoshikuni

    Research output: Contribution to journalArticle

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    Abstract

    The increasing demands placed on natural resources for fuel and food production require that we explore the use of efficient, sustainable feedstocks such as brown macroalgae. The full potential of brown macroalgae as feedstocks for commercial-scale fuel ethanol production, however, requires extensive re-engineering of the alginate and mannitol catabolic pathways in the standard industrial microbe Saccharomyces cerevisiae. Here we present the discovery of an alginate monomer (4-deoxy-l-erythro-5-hexoseulose uronate, or DEHU) transporter from the alginolytic eukaryote Asteromyces cruciatus. The genomic integration and overexpression of the gene encoding this transporter, together with the necessary bacterial alginate and deregulated native mannitol catabolism genes, conferred the ability of an S. cerevisiae strain to efficiently metabolize DEHU and mannitol. When this platform was further adapted to grow on mannitol and DEHU under anaerobic conditions, it was capable of ethanol fermentation from mannitol and DEHU, achieving titres of 4.6% (v/v) (36.2 g l-1) and yields up to 83% of the maximum theoretical yield from consumed sugars. These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks.

    Original languageEnglish (US)
    Pages (from-to)239-243
    Number of pages5
    JournalNature
    Volume505
    Issue number7482
    DOIs
    StatePublished - 2014

    Profile

    Seaweed
    Mannitol
    Ethanol
    Yeasts
    Carbohydrates
    Saccharomyces cerevisiae
    Genes
    Biofuels
    Conservation of Natural Resources
    Eukaryota
    Fermentation
    Food

    ASJC Scopus subject areas

    • General

    Cite this

    Enquist-Newman, M., Faust, A. M. E., Bravo, D. D., Santos, C. N. S., Raisner, R. M., Hanel, A., ... Yoshikuni, Y. (2014). Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature, 505(7482), 239-243. DOI: 10.1038/nature12771

    Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. / Enquist-Newman, Maria; Faust, Ann Marie E; Bravo, Daniel D.; Santos, Christine Nicole S; Raisner, Ryan M.; Hanel, Arthur; Sarvabhowman, Preethi; Le, Chi; Regitsky, Drew D.; Cooper, Susan R.; Peereboom, Lars; Clark, Alana; Martinez, Yessica; Goldsmith, Joshua; Cho, Min Y.; Donohoue, Paul D.; Luo, Lily; Lamberson, Brigit; Tamrakar, Pramila; Kim, Edward J.; Villari, Jeffrey L.; Gill, Avinash; Tripathi, Shital A.; Karamchedu, Padma; Paredes, Carlos J.; Rajgarhia, Vineet; Kotlar, Hans Kristian; Bailey, Richard B.; Miller, Dennis J.; Ohler, Nicholas L.; Swimmer, Candace; Yoshikuni, Yasuo.

    In: Nature, Vol. 505, No. 7482, 2014, p. 239-243.

    Research output: Contribution to journalArticle

    Enquist-Newman, M, Faust, AME, Bravo, DD, Santos, CNS, Raisner, RM, Hanel, A, Sarvabhowman, P, Le, C, Regitsky, DD, Cooper, SR, Peereboom, L, Clark, A, Martinez, Y, Goldsmith, J, Cho, MY, Donohoue, PD, Luo, L, Lamberson, B, Tamrakar, P, Kim, EJ, Villari, JL, Gill, A, Tripathi, SA, Karamchedu, P, Paredes, CJ, Rajgarhia, V, Kotlar, HK, Bailey, RB, Miller, DJ, Ohler, NL, Swimmer, C & Yoshikuni, Y 2014, 'Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform' Nature, vol 505, no. 7482, pp. 239-243. DOI: 10.1038/nature12771
    Enquist-Newman M, Faust AME, Bravo DD, Santos CNS, Raisner RM, Hanel A et al. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature. 2014;505(7482):239-243. Available from, DOI: 10.1038/nature12771

    Enquist-Newman, Maria; Faust, Ann Marie E; Bravo, Daniel D.; Santos, Christine Nicole S; Raisner, Ryan M.; Hanel, Arthur; Sarvabhowman, Preethi; Le, Chi; Regitsky, Drew D.; Cooper, Susan R.; Peereboom, Lars; Clark, Alana; Martinez, Yessica; Goldsmith, Joshua; Cho, Min Y.; Donohoue, Paul D.; Luo, Lily; Lamberson, Brigit; Tamrakar, Pramila; Kim, Edward J.; Villari, Jeffrey L.; Gill, Avinash; Tripathi, Shital A.; Karamchedu, Padma; Paredes, Carlos J.; Rajgarhia, Vineet; Kotlar, Hans Kristian; Bailey, Richard B.; Miller, Dennis J.; Ohler, Nicholas L.; Swimmer, Candace; Yoshikuni, Yasuo / Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform.

    In: Nature, Vol. 505, No. 7482, 2014, p. 239-243.

    Research output: Contribution to journalArticle

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