Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform

Maria Enquist-Newman, Ann Marie E Faust, Daniel D. Bravo, Christine Nicole S Santos, Ryan M. Raisner, Arthur Hanel, Preethi Sarvabhowman, Chi Le, Drew D. Regitsky, Susan R. Cooper, Lars Peereboom, Alana Clark, Yessica Martinez, Joshua Goldsmith, Min Y. Cho, Paul D. Donohoue, Lily Luo, Brigit Lamberson, Pramila Tamrakar, Edward J. Kim & 12 others Jeffrey L. Villari, Avinash Gill, Shital A. Tripathi, Padma Karamchedu, Carlos J. Paredes, Vineet Rajgarhia, Hans Kristian Kotlar, Richard B. Bailey, Dennis J. Miller, Nicholas L. Ohler, Candace Swimmer, Yasuo Yoshikuni

Research output: Contribution to journalArticle

  • 128 Citations

Abstract

The increasing demands placed on natural resources for fuel and food production require that we explore the use of efficient, sustainable feedstocks such as brown macroalgae. The full potential of brown macroalgae as feedstocks for commercial-scale fuel ethanol production, however, requires extensive re-engineering of the alginate and mannitol catabolic pathways in the standard industrial microbe Saccharomyces cerevisiae. Here we present the discovery of an alginate monomer (4-deoxy-l-erythro-5-hexoseulose uronate, or DEHU) transporter from the alginolytic eukaryote Asteromyces cruciatus. The genomic integration and overexpression of the gene encoding this transporter, together with the necessary bacterial alginate and deregulated native mannitol catabolism genes, conferred the ability of an S. cerevisiae strain to efficiently metabolize DEHU and mannitol. When this platform was further adapted to grow on mannitol and DEHU under anaerobic conditions, it was capable of ethanol fermentation from mannitol and DEHU, achieving titres of 4.6% (v/v) (36.2 g l-1) and yields up to 83% of the maximum theoretical yield from consumed sugars. These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks.

LanguageEnglish (US)
Pages239-243
Number of pages5
JournalNature
Volume505
Issue number7482
DOIs
StatePublished - 2014

Profile

Seaweed
Mannitol
Ethanol
Yeasts
Saccharomyces cerevisiae
Biofuels
Eukaryota
Genes
Fermentation
Food
alginic acid

ASJC Scopus subject areas

  • General

Cite this

Enquist-Newman, M., Faust, A. M. E., Bravo, D. D., Santos, C. N. S., Raisner, R. M., Hanel, A., ... Yoshikuni, Y. (2014). Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature, 505(7482), 239-243. DOI: 10.1038/nature12771

Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. / Enquist-Newman, Maria; Faust, Ann Marie E; Bravo, Daniel D.; Santos, Christine Nicole S; Raisner, Ryan M.; Hanel, Arthur; Sarvabhowman, Preethi; Le, Chi; Regitsky, Drew D.; Cooper, Susan R.; Peereboom, Lars; Clark, Alana; Martinez, Yessica; Goldsmith, Joshua; Cho, Min Y.; Donohoue, Paul D.; Luo, Lily; Lamberson, Brigit; Tamrakar, Pramila; Kim, Edward J.; Villari, Jeffrey L.; Gill, Avinash; Tripathi, Shital A.; Karamchedu, Padma; Paredes, Carlos J.; Rajgarhia, Vineet; Kotlar, Hans Kristian; Bailey, Richard B.; Miller, Dennis J.; Ohler, Nicholas L.; Swimmer, Candace; Yoshikuni, Yasuo.

In: Nature, Vol. 505, No. 7482, 2014, p. 239-243.

Research output: Contribution to journalArticle

Enquist-Newman, M, Faust, AME, Bravo, DD, Santos, CNS, Raisner, RM, Hanel, A, Sarvabhowman, P, Le, C, Regitsky, DD, Cooper, SR, Peereboom, L, Clark, A, Martinez, Y, Goldsmith, J, Cho, MY, Donohoue, PD, Luo, L, Lamberson, B, Tamrakar, P, Kim, EJ, Villari, JL, Gill, A, Tripathi, SA, Karamchedu, P, Paredes, CJ, Rajgarhia, V, Kotlar, HK, Bailey, RB, Miller, DJ, Ohler, NL, Swimmer, C & Yoshikuni, Y 2014, 'Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform' Nature, vol. 505, no. 7482, pp. 239-243. DOI: 10.1038/nature12771
Enquist-Newman M, Faust AME, Bravo DD, Santos CNS, Raisner RM, Hanel A et al. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature. 2014;505(7482):239-243. Available from, DOI: 10.1038/nature12771
Enquist-Newman, Maria ; Faust, Ann Marie E ; Bravo, Daniel D. ; Santos, Christine Nicole S ; Raisner, Ryan M. ; Hanel, Arthur ; Sarvabhowman, Preethi ; Le, Chi ; Regitsky, Drew D. ; Cooper, Susan R. ; Peereboom, Lars ; Clark, Alana ; Martinez, Yessica ; Goldsmith, Joshua ; Cho, Min Y. ; Donohoue, Paul D. ; Luo, Lily ; Lamberson, Brigit ; Tamrakar, Pramila ; Kim, Edward J. ; Villari, Jeffrey L. ; Gill, Avinash ; Tripathi, Shital A. ; Karamchedu, Padma ; Paredes, Carlos J. ; Rajgarhia, Vineet ; Kotlar, Hans Kristian ; Bailey, Richard B. ; Miller, Dennis J. ; Ohler, Nicholas L. ; Swimmer, Candace ; Yoshikuni, Yasuo. / Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. In: Nature. 2014 ; Vol. 505, No. 7482. pp. 239-243
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