Emerging affinity-based techniques in proteomics

Shengnan Xie, Colby Moya, Betul Bilgin, Arul Jayaraman, S. Patrick Walton

Research output: Contribution to journalArticle

  • 10 Citations

Abstract

Proteomes of interest, such as the human proteome, have such complexity that no single technique is adequate for the complete analysis of the constituents. Depending on the goal (e.g., identification of a novel protein vs measurement of the level of a known protein), the tools required can vary significantly. While existing methods provide valuable information, their limitations drive the development of complementary, innovative methods to achieve greater breadth of coverage, dynamic range or specificity of analysis. We will discuss affinity-based methods and their applications, focusing on their unique advantages. In addition, we will describe emerging methods with potential value to proteomics, as well as the challenges that remain for proteomic studies.

LanguageEnglish (US)
Pages573-583
Number of pages11
JournalExpert Review of Proteomics
Volume6
Issue number5
DOIs
StatePublished - Oct 2009

Profile

Proteome
Proteomics
Proteins

Keywords

  • Affinity reagent
  • Antibody
  • Aptamer
  • Bead array
  • DNA barcoding
  • Protein-protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Xie, S., Moya, C., Bilgin, B., Jayaraman, A., & Walton, S. P. (2009). Emerging affinity-based techniques in proteomics. Expert Review of Proteomics, 6(5), 573-583. DOI: 10.1586/epr.09.74

Emerging affinity-based techniques in proteomics. / Xie, Shengnan; Moya, Colby; Bilgin, Betul; Jayaraman, Arul; Walton, S. Patrick.

In: Expert Review of Proteomics, Vol. 6, No. 5, 10.2009, p. 573-583.

Research output: Contribution to journalArticle

Xie, S, Moya, C, Bilgin, B, Jayaraman, A & Walton, SP 2009, 'Emerging affinity-based techniques in proteomics' Expert Review of Proteomics, vol. 6, no. 5, pp. 573-583. DOI: 10.1586/epr.09.74
Xie S, Moya C, Bilgin B, Jayaraman A, Walton SP. Emerging affinity-based techniques in proteomics. Expert Review of Proteomics. 2009 Oct;6(5):573-583. Available from, DOI: 10.1586/epr.09.74
Xie, Shengnan ; Moya, Colby ; Bilgin, Betul ; Jayaraman, Arul ; Walton, S. Patrick. / Emerging affinity-based techniques in proteomics. In: Expert Review of Proteomics. 2009 ; Vol. 6, No. 5. pp. 573-583
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