Engineering nanoreactors using bacterial microcompartment architectures

Jefferson S. Plegaria, Cheryl A. Kerfeld

Research output: Research - peer-reviewReview article

Abstract

Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of BMC gene clusters, the production of template empty shells, and the encapsulation of non-native enzymes.

LanguageEnglish (US)
Pages1-7
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume51
DOIs
StatePublished - Jun 1 2018

Profile

Nanoreactors
Enzymes
Proteins
Carbon Monoxide
Encapsulation
Carbon
Genes
Bioengineering
Carbon Cycle
Bacterial Genes
Multigene Family
Organelles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Engineering nanoreactors using bacterial microcompartment architectures. / Plegaria, Jefferson S.; Kerfeld, Cheryl A.

In: Current Opinion in Biotechnology, Vol. 51, 01.06.2018, p. 1-7.

Research output: Research - peer-reviewReview article

@article{801709325fb54830a0bcb2ba87fa658f,
title = "Engineering nanoreactors using bacterial microcompartment architectures",
abstract = "Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of BMC gene clusters, the production of template empty shells, and the encapsulation of non-native enzymes.",
author = "Plegaria, {Jefferson S.} and Kerfeld, {Cheryl A.}",
year = "2018",
month = "6",
doi = "10.1016/j.copbio.2017.09.005",
volume = "51",
pages = "1--7",
journal = "Current Opinion in Biotechnology",
issn = "0958-1669",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Engineering nanoreactors using bacterial microcompartment architectures

AU - Plegaria,Jefferson S.

AU - Kerfeld,Cheryl A.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of BMC gene clusters, the production of template empty shells, and the encapsulation of non-native enzymes.

AB - Bacterial microcompartments (BMCs) are organelles that encapsulate enzymes involved in CO2 fixation or carbon catabolism in a selectively permeable protein shell. Here, we highlight recent advances in the bioengineering of these protein-based nanoreactors in heterologous systems, including transfer and expression of BMC gene clusters, the production of template empty shells, and the encapsulation of non-native enzymes.

UR - http://www.scopus.com/inward/record.url?scp=85031755818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031755818&partnerID=8YFLogxK

U2 - 10.1016/j.copbio.2017.09.005

DO - 10.1016/j.copbio.2017.09.005

M3 - Review article

VL - 51

SP - 1

EP - 7

JO - Current Opinion in Biotechnology

T2 - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

ER -