Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis

Jue Lu, Rankothge Ranjith Weerasiri, Yan Liu, Wei Wang, Shaowen Ji, Ilsoon Lee

Research output: Contribution to journalArticle

  • 10 Citations

Abstract

Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis.

LanguageEnglish (US)
Pages2123-2130
Number of pages8
JournalBiotechnology and Bioengineering
Volume110
Issue number8
DOIs
StatePublished - Aug 2013

Profile

Fungi
Biomass
Zea mays
Hydrolysis
Enzymes
Saccharification
beta-Glucosidase
Cellulase
Fibers
Aspergillus
Ammonia
Microorganisms
Fermentation
Explosions
Electron microscopes
Cells
Scanning
Trichoderma
Acids
Aspergillus niger

Keywords

  • Cellulase
  • Mixed culture
  • Nano-shear pretreatment
  • Whole cell saccharification
  • Xylanase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis. / Lu, Jue; Weerasiri, Rankothge Ranjith; Liu, Yan; Wang, Wei; Ji, Shaowen; Lee, Ilsoon.

In: Biotechnology and Bioengineering, Vol. 110, No. 8, 08.2013, p. 2123-2130.

Research output: Contribution to journalArticle

Lu, Jue ; Weerasiri, Rankothge Ranjith ; Liu, Yan ; Wang, Wei ; Ji, Shaowen ; Lee, Ilsoon. / Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis. In: Biotechnology and Bioengineering. 2013 ; Vol. 110, No. 8. pp. 2123-2130
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