Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis

Jue Lu, Rankothge Ranjith Weerasiri, Yan Liu, Wei Wang, Shaowen Ji, Ilsoon Lee

    Research output: Research - peer-reviewArticle

    • 10 Citations

    Abstract

    Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis.

    LanguageEnglish (US)
    Pages2123-2130
    Number of pages8
    JournalBiotechnology and Bioengineering
    Volume110
    Issue number8
    DOIs
    StatePublished - Aug 2013

    Profile

    Fungi
    Hydrolysis
    Biomass
    Fibers
    Enzymes
    lignocellulose
    Zea mays
    Saccharification
    beta-Glucosidase
    Cellulase
    Aspergillus
    Ammonia
    Microorganisms
    Fermentation
    Explosions
    Electron microscopes
    Cells
    Scanning
    Acids
    Costs

    Keywords

    • Cellulase
    • Mixed culture
    • Nano-shear pretreatment
    • Whole cell saccharification
    • Xylanase

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

    Cite this

    Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis. / Lu, Jue; Weerasiri, Rankothge Ranjith; Liu, Yan; Wang, Wei; Ji, Shaowen; Lee, Ilsoon.

    In: Biotechnology and Bioengineering, Vol. 110, No. 8, 08.2013, p. 2123-2130.

    Research output: Research - peer-reviewArticle

    Lu, Jue ; Weerasiri, Rankothge Ranjith ; Liu, Yan ; Wang, Wei ; Ji, Shaowen ; Lee, Ilsoon. / Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis. In: Biotechnology and Bioengineering. 2013 ; Vol. 110, No. 8. pp. 2123-2130
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