Establishment of a vernalization requirement in Brachypodium distachyon requires REPRESSOR OF VERNALIZATION1

Daniel P. Woods, Thomas S. Ream, Frédéric Bouché, Joohyun Lee, Nicholas Thrower, Curtis Wilkerson, Richard M. Amasino

Research output: Contribution to journalArticle

  • 3 Citations

Abstract

A requirement for vernalization, the process by which prolonged cold exposure provides competence to flower, is an important adaptation to temperate climates that ensures flowering does not occur before the onset of winter. In temperate grasses, vernalization results in the up-regulation of VERNALIZATION1 (VRN1) to establish competence to flower; however, little is known about the mechanism underlying repression of VRN1 in the fall season, which is necessary to establish a vernalization requirement. Here, we report that a plant-specific gene containing a bromo-adjacent homology and transcriptional elongation factor S-II domain, which we named REPRESSOR OF VERNALIZATION1 (RVR1), represses VRN1 before vernalization in Brachypodium distachyon. That RVR1 is upstream of VRN1 is supported by the observations that VRN1 is precociously elevated in an rvr1 mutant, resulting in rapid flowering without cold exposure, and the rapid-flowering rvr1 phenotype is dependent on VRN1. The precocious VRN1 expression in rvr1 is associated with reduced levels of the repressive chromatin modification H3K27me3 at VRN1, which is similar to the reduced VRN1 H3K27me3 in vernalized plants. Furthermore, the transcriptome of vernalizedwild-type plants overlapswith that of nonvernalized rvr1 plants, indicating loss of rvr1 is similar to the vernalized state at a molecular level. However, loss of rvr1 results in more differentially expressed genes than does vernalization, indicating that RVR1 may be involved in processes other than vernalization despite a lack of any obvious pleiotropy in the rvr1 mutant. This study provides an example of a role for this class of plant-specific genes.

LanguageEnglish (US)
Pages6623-6628
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number25
DOIs
StatePublished - Jun 20 2017

Profile

Brachypodium distachyon
vernalization
flowering
flowers
mutants
pleiotropy
genes
temperate zones
transcriptome
chromatin
autumn
grasses
phenotype
winter

Keywords

  • Brachypodium
  • Flowering
  • RVR1
  • Vernalization
  • VRN1

ASJC Scopus subject areas

  • General

Cite this

Establishment of a vernalization requirement in Brachypodium distachyon requires REPRESSOR OF VERNALIZATION1. / Woods, Daniel P.; Ream, Thomas S.; Bouché, Frédéric; Lee, Joohyun; Thrower, Nicholas; Wilkerson, Curtis; Amasino, Richard M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 25, 20.06.2017, p. 6623-6628.

Research output: Contribution to journalArticle

Woods, Daniel P. ; Ream, Thomas S. ; Bouché, Frédéric ; Lee, Joohyun ; Thrower, Nicholas ; Wilkerson, Curtis ; Amasino, Richard M./ Establishment of a vernalization requirement in Brachypodium distachyon requires REPRESSOR OF VERNALIZATION1. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 25. pp. 6623-6628
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